Spectral 5-Laser Cell Sorter

光谱 5 激光细胞分选仪

• 批准号: 528301049
• 金额: --
• 依托单位: Rheinische Friedrich-Wilhelms-Universität Bonn
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/528301049?language=en
• 关键词: Spectral; Laser; Cell; Sorter

As an extension to the existing equipment of the Flow Cytometry Core Facility of the Medical Department of the University Bonn, a high-parameter, spectral, high-speed cell sorting system housed in a biosafety cabinet is to be procured. Cell sorting requirements have increased steadily in the Flow Cytometry Core Facility over the past several years. With the acquisition of a Spectral Analytical Flow Cytometer in early 2022, the demand for cell sorting beyond 20 parameters is increasing tremendously. Currently, the Core Facility has only one cell sorting system, which is housed in a biosafety cabinet and equipped with 5 excitation lasers, that can sort a maximum of 20 parameters. This device was purchased primarily for sorting cells of biosafety level 2, is located in an S2 laboratory, and is largely utilized solely for sorting organisms of biosafety level 2. For already existing and future planned research projects from different disciplines an additional device is needed, which is able to sort more than 20 parameters, equipped with a UV excitation laser, able to process spectral data and which is able to collect up to 6 target populations. Furthermore, it is necessary that the device is housed in a biosafety workbench so that untested, human samples can also be sorted.

作为波恩大学医学系流式细胞术核心设施现有设备的延伸，将采购一套安装在生物安全柜内的高参数、光谱、高速细胞分选系统。在过去的几年中，流式细胞术核心设备的细胞分选需求稳步增加。随着光谱分析流式细胞仪在2022年初的收购，对超过20个参数的细胞分选的需求正在急剧增加。目前，核心设施只有一个细胞分选系统，它被安置在一个生物安全柜里，配备了5个激发激光器，最多可以分选20个参数。本设备主要用于生物安全等级2的细胞分选，位于S2实验室，主要用于生物安全等级2的生物分选。对于已经存在的和未来计划的来自不同学科的研究项目，需要一个额外的设备，它能够对20多个参数进行分类，配备有紫外线激发激光器，能够处理光谱数据，并且能够收集多达6个目标人群。此外，该设备必须安装在生物安全工作台上，以便对未经测试的人类样本进行分类。