5-Laser Cell Sorter

基于细胞分选的细胞分选是一种强大的技术，用于根据细胞的物理和生化特性分离和隔离细胞。该方法结合了流式细胞术和荧光显微镜的原理，能够将细胞的异质混合物分选成不同的群体。该技术依赖于使用荧光探针标记细胞和使用激光束激发荧光分子，从而以高通量方式识别和分离单个细胞。由于其广泛的适用性，该提案汇集了五个研究小组，其兴趣范围从生物化学和遗传学到生物力学，生物技术和材料科学。我们计划利用该仪器从主要来源分离大量全能干细胞用于再生研究。这将使我们能够阐明干细胞功能所必需的基因调控机制。同时，该仪器的单细胞分离能力将产生细胞间变异的分析。在人类细胞的单独项目中，我们将利用该仪器在慢病毒转导实验后选择克隆。这种实验能力是至关重要的，以推进我们的利益在细胞分裂和真核转录调控过程中的遗传信息的稳态。此外，该仪器将允许将荧光标记分子引入靶细胞。这些转导的细胞随后被用于研究调节细胞粘附、迁移和分化的力，另一方面，这些力对于将联合收割机工程方法与3D材料的纳米织物相结合至关重要。此外，生物启发材料的生产和应用需要使用细胞分选。这是因为原代细胞在用于组织工程和再生之前需要分离。此外，我们使用细胞分选来工程化难以使用经典方法操作的原代细胞。最后，我们将利用该仪器来研究聚合物和其他粒子与细胞的相互作用。我们这样做是为了破译它们在器官打印和组织工程过程中的相互作用，并研究它们从环境中吸收到细胞中。总的来说，这里申请的高性能仪器将加强拜罗伊特大学现有的研究领域。然而，最重要的是，它将使我们能够开辟全新的研究领域，这些领域目前由于缺乏类似的工具而无法进入。

Fluorescence-based cell sorting is a powerful technique used to separate and isolate cells based on their physical and biochemical properties. The method combines principles of flow cytometry and fluorescence microscopy to enable the sorting of a heterogeneous mixture of cells into distinct populations. The technique relies on the use of fluorescent probes to label cells and the use of a laser beam to excite the fluorescent molecules, allowing for the identification and separation of individual cells in a high-throughput manner. Due to its broad applicability, this proposal brings together five research groups whose interests range from biochemistry and genetics to biomechanics, biotechnology, and materials science. We plan to utilize the instrument to isolate large quantities of totipotent stem cells for regeneration research from a primary source. This will allow us to elucidate gene regulatory mechanisms that are essential for stem cell function. Concomitantly, the single-cell isolation capability of the instrument will spawn the analysis of cell-to-cell variation. In separate projects in human cells, we will utilize the instrument to select clones following lentiviral transduction experiments. This experimental capability is vital to advance our interests in the homeostasis of genetic information during cell division and eukaryotic transcription regulation. Furthermore, the instrument will allow for the introduction of fluorescently labelled molecules into target cells. These transduced cells are subsequently used to study the forces that regulate cell adhesion, migration and differentiation, and, on the other hand essential to combine engineering approaches with the nanofabrication of 3D-materials. In addition, the production and application of bioinspired materials requires the use of cell sorting. This is the case as primary cells need to be isolated prior to their usage in tissue engineering and regeneration. Moreover, we use cell sorting to engineer primary cells that are difficult to manipulate using classical methods. Last, we will utilize the instrument to study the interaction of polymers and other particles with cells. We do so to both decipher their interplay during organ printing and tissue engineering and to study their uptake into cells from the environment. Overall, the high-performance instrument applied for here will strengthen existing lines of research at the University of Bayreuth. Above all, however, it will enable us to open up entirely new fields of research that are currently inaccessible for lack of a comparable instrument.