5-Laser Cell Sorter

5 激光细胞分选仪

• 批准号: 531942116
• 金额: --
• 依托单位: Universität Bayreuth
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/531942116?language=en
• 关键词: Laser; Cell; Sorter

Fluorescence-based cell sorting is a powerful technique used to separate and isolate cells based on their physical and biochemical properties. The method combines principles of flow cytometry and fluorescence microscopy to enable the sorting of a heterogeneous mixture of cells into distinct populations. The technique relies on the use of fluorescent probes to label cells and the use of a laser beam to excite the fluorescent molecules, allowing for the identification and separation of individual cells in a high-throughput manner. Due to its broad applicability, this proposal brings together five research groups whose interests range from biochemistry and genetics to biomechanics, biotechnology, and materials science. We plan to utilize the instrument to isolate large quantities of totipotent stem cells for regeneration research from a primary source. This will allow us to elucidate gene regulatory mechanisms that are essential for stem cell function. Concomitantly, the single-cell isolation capability of the instrument will spawn the analysis of cell-to-cell variation. In separate projects in human cells, we will utilize the instrument to select clones following lentiviral transduction experiments. This experimental capability is vital to advance our interests in the homeostasis of genetic information during cell division and eukaryotic transcription regulation. Furthermore, the instrument will allow for the introduction of fluorescently labelled molecules into target cells. These transduced cells are subsequently used to study the forces that regulate cell adhesion, migration and differentiation, and, on the other hand essential to combine engineering approaches with the nanofabrication of 3D-materials. In addition, the production and application of bioinspired materials requires the use of cell sorting. This is the case as primary cells need to be isolated prior to their usage in tissue engineering and regeneration. Moreover, we use cell sorting to engineer primary cells that are difficult to manipulate using classical methods. Last, we will utilize the instrument to study the interaction of polymers and other particles with cells. We do so to both decipher their interplay during organ printing and tissue engineering and to study their uptake into cells from the environment. Overall, the high-performance instrument applied for here will strengthen existing lines of research at the University of Bayreuth. Above all, however, it will enable us to open up entirely new fields of research that are currently inaccessible for lack of a comparable instrument.

基于荧光的细胞分选是一种强大的技术，用于根据细胞的物理和生化特性来分离和分离细胞。该方法结合了流式细胞术和荧光显微镜的原理，能够将不同种类的细胞混合物分选成不同的群体。这项技术依赖于使用荧光探针标记细胞，并使用激光激发荧光分子，从而以高通量的方式识别和分离单个细胞。由于其广泛的适用性，这项建议汇集了五个研究小组，他们的兴趣范围从生物化学和遗传学到生物力学、生物技术和材料科学。我们计划利用该仪器从原始来源分离大量全能干细胞，用于再生研究。这将使我们能够阐明对干细胞功能至关重要的基因调控机制。随之而来的是，该仪器的单细胞分离能力将产生细胞间变异的分析。在人体细胞的不同项目中，我们将在慢病毒转导实验后利用该仪器选择克隆。这种实验能力对于提高我们对细胞分裂和真核转录调控过程中遗传信息的动态平衡的兴趣至关重要。此外，该仪器将允许将荧光标记的分子引入靶细胞。这些被转换的细胞随后被用来研究调节细胞黏附、迁移和分化的力，另一方面，对于将工程方法与3D材料的纳米制造相结合是必不可少的。此外，生物灵感材料的生产和应用需要使用细胞分选。这是因为原代细胞在用于组织工程和再生之前需要被分离。此外，我们使用细胞分选来设计使用经典方法难以操作的原代细胞。最后，我们将利用该仪器研究聚合物和其他粒子与细胞的相互作用。我们这样做既是为了破译它们在器官打印和组织工程中的相互作用，也是为了研究它们从环境中吸收到细胞中。总体而言，这里申请的高性能仪器将加强拜罗伊特大学现有的研究方向。然而，最重要的是，它将使我们能够开辟目前由于缺乏可比工具而无法进入的全新研究领域。