Construction of Non-heme Enzyme Models and Their Functions

非血红素酶模型的构建及其功能

• 批准号: 09440230
• 负责人: MATSUDA Hideki
• 金额: $ 8.64万
• 依托单位: Nagoya Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09440230/
• 关键词: small molecule activation; Oxygen species; Amino acids; Self-organization; Unique reaction system; Non-covalent interaction; Non-heme enzyme model; 活性酸素; 酸素錯体; 金属錯体; 部位特異的分子認識; ATPase; ビタミンB6; 金属酵素; 酵素機能; 生体系特殊反応場; 非ヘム酵素; モデル錯体; 銅錯体; 鉄錯

Some novel structural and/or functional model complexes for metalloenzymes were constructed, which have synthetically coordinated non-covalent interaction groups such as hydrogen bonding, hydrophobic, electrostatic, and π-π stacking interactions. 1. Fundamental analysis of such weak interactions were studied and the principle of self-organization was examined. 2. The metal complexes that enable to recognize amino acids and peptides were constructed as a peptidase model. 3. Transamination was examined by the use of Co(III)-pyridoxal-amino acids under non-enzymatic conditions. 4. Carbonic anhydrase model was constructed by the use of Zn(II)-polypyridylamine ligands. 5. The organic compounds recognizing and capturing the biologically essential metal elements were prepared. 6. The oxidase models that enable to recognize and activate oxygen species were constructed and some reaction intermediates with oxygen species were prepared and examined physico-chemically. 7. Hydrogenase models were synthesized by the use of Ni(II)-NィイD22ィエD2SィイD22ィエD2 complexes that regulate the redox potential.

一些新型的金属酶结构和功能模型配合物被构建出来，它们具有合成配位的非共价相互作用基团，如氢键、疏水、静电和π-π堆积相互作用。1.研究了这种弱相互作用的基本分析，并检验了自组织原理。2.构建了能够识别氨基酸和肽的金属配合物作为肽酶模型。3.在非酶促条件下，通过使用Co（III）-吡哆醛-氨基酸检查转氨酶。4.碳酸酐酶模型是通过使用锌（II）-多吡啶胺配体构建的。5.制备了识别和捕获生物必需金属元素的有机化合物。6.建立了能够识别和活化氧的氧化酶模型，制备了一些与氧反应的中间产物，并对其进行了理化性质的研究。7.氢化酶模型的合成通过使用Ni（II）-N-[22]-[23]-[24]-[25]-[26]-[27]-[28]-[29]-[2

项目成果

H. Masuda: "Zn(II)-OH Complexes with Tripodal Tetradentate Ligands as a Functional Model of Carbonic Anhydrase"J. Inorg. Biochem.. 74. 225 (1999)

H. Masuda：“具有三齿四齿配体的 Zn(II)-OH 复合物作为碳酸酐酶的功能模型”J。

H. Kitamura: "Syntheses, Structures, and Properties of Tetrakis(m-acetato)-dirhodium(II) Complexes with Axial Pyridine Nitrogen-Donor Ligands with and/or without Assistance of Hydrogen Bonds"Inorg. Chem.. (印刷中). (2000)

H. Kitamura：“具有和/或不具有氢键辅助的轴向吡啶氮供体配体的四(间-乙酰基)-二铑(II)配合物的合成、结构和性质”Inorg. (2000)

N. Ohata, H. Masuda and O. Yamauchi: "Process of Self-Organization of Metal(II) Arginine-Dianion Systems in Solution. A NMR Study."J. Inorg. Biochem.. 67. 452 (1997)

N. Ohata、H. Masuda 和 O. Yamauchi：“溶液中金属 (II) 精氨酸-双阴离子系统的自组织过程。核磁共振研究。”J.

A. Wada, M. Harata, K. Hasegawa, K. Jitsukawa, H. Masuda, M. Mukai, T. Kitagawa, and H. Einaga: "Structural and Spectroscopic Characterization of Hydroperoxo Mononuclear Copper Complex with Tripodal Pyridylamine Ligands"Angew. Chem., Int. Ed. Engl.. 37. 7

A. Wada、M. Harata、K. Hasekawa、K. Jitsukawa、H. Masuda、M. Mukai、T. Kitakawa 和 H. Einaga：“具有三足吡啶胺配体的氢过氧单核铜络合物的结构和光谱表征”Angew。

M. Mizutani, N. Maejima, K. Jitsukawa, H. Masuda, and H. Einaga: "An infinite Chiral Single-helical Structure formed in Cu(II)-L-/D-glutamic Acid System"Inorg. Chem. Acta.. 298. 105-110 (1998)

M. Mizutani、N. Maejima、K. Jitsukawa、H. Masuda 和 H. Einaga：“Cu(II)-L-/D-谷氨酸系统中形成的无限手性单螺旋结构”Inorg。