Construction of Non-heme Enzyme Models and Their Functions

点击翻译按钮获取中文摘要

Some novel structural and/or functional model complexes for metalloenzymes were constructed, which have synthetically coordinated non-covalent interaction groups such as hydrogen bonding, hydrophobic, electrostatic, and π-π stacking interactions. 1. Fundamental analysis of such weak interactions were studied and the principle of self-organization was examined. 2. The metal complexes that enable to recognize amino acids and peptides were constructed as a peptidase model. 3. Transamination was examined by the use of Co(III)-pyridoxal-amino acids under non-enzymatic conditions. 4. Carbonic anhydrase model was constructed by the use of Zn(II)-polypyridylamine ligands. 5. The organic compounds recognizing and capturing the biologically essential metal elements were prepared. 6. The oxidase models that enable to recognize and activate oxygen species were constructed and some reaction intermediates with oxygen species were prepared and examined physico-chemically. 7. Hydrogenase models were synthesized by the use of Ni(II)-NィイD22ィエD2SィイD22ィエD2 complexes that regulate the redox potential.