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PREDICTION OF RADIOTHERAPEUTIC EFFECTS ON TUMOR USING POSITRON EMISSION TOMOGRAPHY

使用正电子发射断层扫描预测肿瘤放射治疗效果

基本信息

批准号：
09470195
负责人：
KUBOTA Kazuo
金额：
$ 7.74万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

To evaluate tumor imaging potential of 18F-fluoromisonidazole (FMISO), we studied FMISO uptake in an experimental tumor model and examined the correlation between intra-tumoral distributions of FMISO, 14C-2-deoxyglucose (2DG) and 14C-methionine (Met). Methods: We studied the issue distribution and double tracer autoradiography using control rats with AH109A tumor and rats with the same tumor under local hypoxia. Results: Tumor uptake of FMISO was constant between 30 min to 2 hr after injection, and the tumor to muscle ratio was 2, from 2 to 4 hr. A tumor study with FMISO was scheduled at 2 hr. Double tracer autoradiography of the tumor demonstrated that in the areas of high FMISO uptake, there was low uptake of Met, while low FMISO uptake areas showed high Met uptake. FMISO showed high grain density in the rim of the tumor surrounding the necrotic area. 2DG showed more uniform distribution over the entire section of viable cells. The mean uptake of FMISO by hypoxic, radioresistant tumors was significantly higher than the control tumors (p<0.05), while both 2DG and Met uptake by the control tumor were higher than hypoxic tumor. When individual tumors were examined, the uptake of FMISO was inversely correlated with that of Met (r=-0.507, p<0.02), while 2DG showed almost uniform uptake with no significant correlation to FMISO. Conclusions: Hypoxic and radioresistant tumour can be identified by increased FMISO uptake in our model consistent with works reported by others. We found a large overlap in the distribution of FMISO and 2DG within the tumor, but only a small overlap in the distribution of FMISO and Met. A combination of FMISO and other tracers in PET or SPECT study would possibly be more helpful than single tracer study in predicting the response of tumor tissues to radiotherapy.

为了评估 18F-氟米索硝唑 (FMISO) 的肿瘤成像潜力，我们研究了实验肿瘤模型中 FMISO 的摄取，并检查了 FMISO、14C-2-脱氧葡萄糖 (2DG) 和 14C-甲硫氨酸 (Met) 的肿瘤内分布之间的相关性。方法：我们使用具有 AH109A 肿瘤的对照大鼠和具有相同肿瘤的大鼠在局部缺氧下研究了问题分布和双示踪放射自显影。结果：注射后 30 分钟至 2 小时内，肿瘤对 FMISO 的摄取保持恒定，2 至 4 小时内，肿瘤与肌肉的比率为 2。计划在 2 小时进行 FMISO 肿瘤研究。肿瘤的双示踪放射自显影显示，在高 FMISO 摄取区域，Met 摄取较低，而低 FMISO 摄取区域则显示高 Met 摄取。 FMISO 显示坏死区域周围肿瘤边缘的颗粒密度较高。 2DG 在整个活细胞切片上显示出更均匀的分布。低氧、抗放射肿瘤对 FMISO 的平均摄取显着高于对照肿瘤 (p<0.05)，而对照肿瘤对 2DG 和 Met 的摄取均高于低氧肿瘤。当检查单个肿瘤时，FMISO的摄取与Met的摄取呈负相关（r=-0.507，p<0.02），而2DG的摄取几乎一致，与FMISO没有显着相关性。结论：在我们的模型中，可以通过增加 FMISO 摄取来识别缺氧和放射抗性肿瘤，这与其他人报道的工作一致。我们发现肿瘤内 FMISO 和 2DG 的分布有很大的重叠，但 FMISO 和 Met 的分布只有很小的重叠。在 PET 或 SPECT 研究中，FMISO 与其他示踪剂的组合可能比单一示踪剂研究更有助于预测肿瘤组织对放射治疗的反应。