Development of cancer imaging correlated to metastatic potential using positron emission tomography

使用正电子发射断层扫描技术开发与转移潜能相关的癌症成像

• 批准号: 04557047
• 负责人: KUBOTA Kazuo
• 金额: $ 3.26万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04557047/
• 关键词: Positron emission tomography; Tumor diagnosis; Tumor imaging; Metastatic potential; Hyaluronic acid metabolism; N-[^<18>F]fluoroacetyl-D-glucosamine; ^<18>F-labeled amino acid; ^<18>Fフルオロフェニルアラニン; 癌転移; 腫瘍核医学; FM3A乳癌; B16メラノーマ; 18Fフルオロドーパ; 18F

We have performed experimental studies for three years aiming to develop a cancer imaging correlated to metastatic potential, or malignant potential using positron emission tomography.In order to investigate the metastatic potential of tumors in vivo bymeasuring hyaluronic acid metabolism, C57BL/6 mice with B16 melanoma variants and C3H/He mice with FM3A tumor variants were evaluated using N-[^<18>F]fluoroacetyl-D-glucosamine ([^<18>F]GluNFAc) . The uptake of[^<18>F]GluNFAc was slightly higher in high-metastatic variant, B16-F10, than in low-metastatic one, B16-F1 (p<0.05) . However, it is difficult to detect the difference of each tumor uptake using autoradiography. Analysis of metabolites suggested the slower metabolism in tumors than that in liver.In order to evaluate the malignant potential of tumors in vivo using ^<18>F labeled amino acids or its analogs, 3,4-dihydroxy-2-[^<18>F]fluoro-L-phenylalanine (2-[^<18>F]DOPA) was studied for melanomas imaging, L-2-[^<18>F]fluoro-tyrosine ([^<18>F]Tyr) for a marker of protein synthesis, and L-[2-^<18>F]fluorophenylalanine ([^<18>F]FPhe) for a marker of amino acid transport.

我们已经进行了三年的实验研究，旨在利用正电子发射断层扫描开发与转移潜能或恶性潜能相关的癌症成像。为了通过测量透明质酸代谢来研究肿瘤在体内的转移潜力，我们使用N-[^<18>F]氟乙酰- d -葡萄糖胺（[^<18>F]GluNFAc）对B16黑色素瘤变异的C57BL/6小鼠和FM3A肿瘤变异的C3H/He小鼠进行了评估。[^<18>F]GluNFAc在高转移型B16-F10中的摄取略高于低转移型B16-F1 （p<0.05）。然而，使用放射自显像很难检测到每个肿瘤摄取的差异。代谢物分析表明肿瘤的代谢比肝脏慢。为了评估体内肿瘤使用^< 18>f标记的氨基酸或其类似物的恶性潜能，研究了3,4-二羟基-2-[^< 18>f]氟-L-苯丙氨酸（2-[^< 18>f]DOPA）用于黑色素瘤成像，L-2-[^< 18>f]氟酪氨酸（[^< 18>f]Tyr）作为蛋白质合成标记物，L-[2-^< 18>f]氟苯丙氨酸（[^< 18>f]FPhe）作为氨基酸运输标记物。

项目成果

Kubota R,Kubota K,Yamada S,Tada M,Ido T,Tamahashi N: "Active and passive mechanisms of[fluorine-18]fluorodeoxyglucose uptake by proliferating and prenecrotic cancer cells in vivo : a microautoradiographic study" J.Nucl.Med.35. 1067-1075 (1994)

Kubota R，Kubota K，Yamada S，Tada M，Ido T，Tamahashi N：“体内增殖和坏死前癌细胞摄取[氟-18]氟脱氧葡萄糖的主动和被动机制：一项显微放射自显影研究”J.Nucl.Med。

Yamada S,Kubota K,Kubota R,Tamahashi N: "Quantitative microautoradigraphic study of 18F-FDG in experimental inflammatory tissue." CYRIC Annual Report Tohoku University. 93. 150-154 (1994)

Yamada S、Kubota K、Kubota R、Tamahashi N：“实验性炎症组织中 18F-FDG 的定量显微自放射学研究。”

R.Kubota,S.Yamada,K.Ishiwata,K.Kubota,T.Ido: "Active melanogenesis in non-S phase melanocytes in B16 melanomas in vivo investigated by double-tracer microautoradiography with ^<18>F-fluorodopa and ^3H-thymidine" British Journal of Cancer. 66. 614-618 (199

R.Kubota、S.Yamada、K.Ishiwata、K.Kubota、T.Ido：“体内 B16 黑色素瘤中非 S 期黑色素细胞的活性黑色素生成通过使用^18F-氟多巴和^的双示踪剂显微放射自显影技术进行研究

Tada M,Iwata R,Sugiyama H,Sato K,Fukuda H,Kubota K,Kubota R,Fujiwara T,Takahashi H,Wakui A,Ido T.: "An efficient synthesis of Nw-[^<18>F]fluoroacetylserotonin." J.Labelled Compds.Radiopharm.34. 741-746 (1994)

Tada M，Iwata R，Sugiyama H，Sato K，Fukuda H，Kubota K，Kubota R，Fujiwara T，Takahashi H，Wakui A，Ido T.：“Nw-[^ 18 F]氟乙酰血清素的有效合成。

S.Yamada,R.Kubota,K.Kubota,K.Ishiwata,et al.: "Efficiency of grain production and latent image fading in F-18 microautoradiography" Tohoku Journal of Experimental Medicine. 167. 181-184 (1992)

S.Yamada，R.Kubota，K.Kubota，K.Ishiwata，et al.：“F-18显微放射自显影中谷物生产的效率和潜像褪色”东北实验医学杂志。