Development of cancer imaging correlated to metastatic potential using positron emission tomography

为了通过透明质酸代谢来研究体内肿瘤的转移潜力，我们进行了三年的实验研究，目的是利用正电子发射断层扫描技术开发与转移潜力或恶性潜力相关的癌症成像，使用N-[^ F]氟乙酰基-D-葡萄糖胺（[^F]GluNFAc）评估患有B16黑素瘤变体的C57 BL/6小鼠和患有FM 3A肿瘤变体的C3 H/He小鼠<18><18>。在<18>高转移性变异体B16-F10中，[^ F]GluNFAc的摄取略高于低转移性变异体B16-F1（p&lt;0.05）。然而，使用放射自显影难以检测每个肿瘤摄取的差异。为了使用13 <18>F标记的氨基酸或其类似物在体内评估肿瘤的恶性潜能，研究了用于黑色素瘤成像的3，4-二羟基-2-[13 <18>F]氟-L-苯丙氨酸（2-[13 <18>F]DOPA）、用于蛋白质合成标记的L-2-[13 <18>F]氟-酪氨酸（[13 <18>F]Tyr）和用于氨基酸转运标记的L-[13 <18>F]氟苯丙氨酸（[13 <18>F]FPhe）。

We have performed experimental studies for three years aiming to develop a cancer imaging correlated to metastatic potential, or malignant potential using positron emission tomography.In order to investigate the metastatic potential of tumors in vivo bymeasuring hyaluronic acid metabolism, C57BL/6 mice with B16 melanoma variants and C3H/He mice with FM3A tumor variants were evaluated using N-[^<18>F]fluoroacetyl-D-glucosamine ([^<18>F]GluNFAc) . The uptake of[^<18>F]GluNFAc was slightly higher in high-metastatic variant, B16-F10, than in low-metastatic one, B16-F1 (p<0.05) . However, it is difficult to detect the difference of each tumor uptake using autoradiography. Analysis of metabolites suggested the slower metabolism in tumors than that in liver.In order to evaluate the malignant potential of tumors in vivo using ^<18>F labeled amino acids or its analogs, 3,4-dihydroxy-2-[^<18>F]fluoro-L-phenylalanine (2-[^<18>F]DOPA) was studied for melanomas imaging, L-2-[^<18>F]fluoro-tyrosine ([^<18>F]Tyr) for a marker of protein synthesis, and L-[2-^<18>F]fluorophenylalanine ([^<18>F]FPhe) for a marker of amino acid transport.