Development of therapeutic ways on various pathological features in chronic obstructive pulmonary diseases

慢性阻塞性肺疾病多种病理特征治疗方法的进展

• 批准号: 09470526
• 负责人: MIYATA Takeshi
• 金额: $ 9.54万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470526/
• 关键词: LYSOZYME; MEF; LUNG; EPITHELIUM; 麦門冬湯; 肺サーファクタント; 気道; 咳; グリシン; 上皮細胞; リゾチーム; アポトーシス; 肺胞上皮細胞; 分化

We have published about 20 publications in International Journal. The findings in this study were summarized below.1. Dextromethorphan inhibited glycine currents in neurons dissociated from the guinea-pig nucleus tractus solitarii.2. Human neutrophil elastase inhibited tracheala mucociliary transport in anesthetized quails.3. MEF most strongly stimulated lysozyme transcription in lung epithelial cells (A549), colon carcinoma cells (Caco2), and skin fibroblasts (NIH3T3). These studies provide the first information identifying a transcription factor controlling lysozyme expression in epithelial cells.4. Maimendongtang improved decreased airway clearance and abnormal secretion.5. Pulmonary surfactant secretion was regulated by various drugs and endogenous factors in type II pneumocytes.

我们在国际期刊上发表了约20篇论文。本研究的结果总结如下。右美沙芬抑制豚鼠孤束核神经元的甘氨酸电流.人中性粒细胞弹性蛋白酶抑制麻醉鹌鹑气管粘膜纤毛转运. MEF最强烈地刺激肺上皮细胞（A549）、结肠癌细胞（Caco 2）和皮肤成纤维细胞（NIH 3T3）中的溶菌酶转录。这些研究提供了第一个信息，确定了一个转录因子控制溶菌酶在上皮细胞中的表达。脉门冬汤能改善气道清除率降低及异常分泌物.肺表面活性物质的分泌受多种药物和内源性因子的调节。

项目成果

H.Kai: "MEF up-regulates lysozyme transcription in epithelial cells."J.Biol.Chem.. 274. 20098-20102 (1999)

H.Kai：“MEF 上调上皮细胞中的溶菌酶转录。”J.Biol.Chem.. 274. 20098-20102 (1999)

Y. Isohama, Y. Kumanda, K. Tanaka, H. Kai, K. Takahama and T. Miyata: "Dexamethasone increases βィイD22ィエD2-adrenoceptor-regulated phosphatidylcholine secretion in rat alveolar type II cells"Jpn. J. Pharmacol.. 73. 163-169 (1997)

Y. Isohama、Y. Kumanda、K. Tanaka、H. Kai、K. Takahama 和 T. Miyata：“地塞米松增加大鼠 II 型肺泡细胞中 β-D22-肾上腺素受体调节的磷脂酰胆碱分泌”Jpn Pharmacol. 73. 163-169 (1997)

S.Nagayama: "Characterization of CFTR expression in a human pulmonary mucoepidermoid carcinoma cell line,NCIH292 cells"FEBS Letter. 455. 215-218 (1999)

S.Nagayama：“人肺粘液表皮样癌细胞系 NCIH292 细胞中 CFTR 表达的表征”FEBS Letter。

S. Tai, H. Kai, T. Kido, Y. Isohama, K. Takahama and T. Miyata: "Effect of human neutrophil elastase on tracheal mucociliary transport in anesthetized quails"Jpn. J. Pharmacol.. 75. 350-355 (1997)

S. Tai、H. Kai、T. Kido、Y. Isohama、K. Takahama 和 T. Miyata：“人中性粒细胞弹性蛋白酶对麻醉鹌鹑气管粘膜纤毛运输的影响”Jpn。

S. Tai, H. Kai, Y. Isohama, H. Moriuchi, N. Hagino and T. Miyata: "The effect of Maimendongtang on airway clearance and secretion"Phytotherapy. Res.. 13. 124-127 (1999)

S. Tai、H. Kai、Y. Isohama、H. Moriuchi、N. Hagino 和 T. Miyata：“麦门冬汤对气道清除和分泌的影响”植物疗法。