Development of new drug delivery system for jaw infection

开发治疗颌部感染的新型给药系统

• 批准号: 09557168
• 负责人: MATSUMOTO Ken
• 金额: $ 7.1万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557168/
• 关键词: osteomyelitis; mandible; drug delivery system; hydroxyapatite block; 下顎骨骨髄炎; 顎骨; 炎症; 抗菌剤; DDS

We have treated the patients with diffuse sclerosing osteomyelitis of the mandible using antibiotic-impregnated polymethylmethacrylate (PMMA) beads. This drug delivery system provides sustained release of antibiotics in mandible without systemic side effects. In this research, we designed new drug delivery system using porous hydroxyapatite block (HAB), which is safer and more biocompatible than PMMA. HAB loaded with antibiotics or anticancer drugs have recently developed for the treatment of the osteomyelitis or the malignant tumor of the extremities. Those ready-made HAB are not suitable for the mandible in size and shape. We designed a new original HAB which fit to the mandible after the sequestrectomy and the decortication. This HAB will be served as a bone replacement material after the control of infection. A cylindrical cavity of the new HAB was filled with powder of antibiotics. The release of gentamicin from HAB was more than PMMA in vitro examination untill day4, same amount of gentamicin was released from HAB and PMMA after day7. We implanted this new HABs enclosing antibiotics to the mandible of goats, and after the surgery no significant postoperative complication was observed. We are going to explore the union of the HAB and the bone and measure the concentration of the antibiotics in the surrounding tissues.

我们已经治疗的患者弥漫性硬化性骨髓炎的下颌骨使用丙烯酸树脂浸渍聚甲基丙烯酸甲酯（PMMA）珠。这种药物递送系统提供了在下颌骨中持续释放抗生素而没有全身副作用。在本研究中，我们设计了一种新的药物释放系统，使用多孔羟基磷灰石块（HAB），这是比PMMA更安全和更好的生物相容性。最近开发了装载有抗生素或抗癌药物的HAB，用于治疗骨髓炎或四肢恶性肿瘤。现有的HAB在大小和形状上都不适合下颌骨。我们设计了一种新的原始HAB，适合于在死骨切除和去皮质后的下颌骨。控制感染后，将其作为骨替代材料。新的HAB的圆柱形腔室填充有抗生素粉末。在体外实验中，HAB的庆大霉素释放量一直到第4天才大于PMMA，第7天后两者的庆大霉素释放量基本相同。我们将这种包封抗生素的新型HAB植入山羊下颌骨，术后没有观察到明显的术后并发症。我们将探索HAB和骨头的结合并测量周围组织中抗生素的浓度。

项目成果

A. Matsumoto: "Bacteria isolated from dental infectioin-Comparison between two laboratories"J Osaka Univ Dent Soc. 43(2). 57-62 (1998)

A. Matsumoto：“从牙科感染中分离出的细菌 - 两个实验室之间的比较”J Osaka Univ Dent Soc。

植草康浩、松本憲 他: "Congenital submental salivary fistula -letter to the editor-"Int. J.Oral Maxillofac Surg. 28. 400 (1999)

Yasuhiro Uekusa、Ken Matsumoto 等人：“先天性颏下唾液瘘 - 致编辑的信” Int. Oral Maxillofac Surg。 28. 400 (1999)

児玉裕美子、松本 憲 他: "外来顎関節鏡検査に関する患者アンケート調査 第2報" 阪大学歯学雑誌. 42(2). 213-218 (1997)

Yumiko Kodama、Ken Matsumoto 等人：“关于门诊颞下颌关节镜检查的患者问卷调查，第 2 次报告”，大阪大学牙科杂志 42(2) (1997)。

Y. Atsumi, K. Matsumoto et al.: "Altered distribution of Svhwann cells in the periodontal ligament of the rat incisor following resection of the inferior alveolar nerve : an immunohistochemical study on S-100 proteins"Brain Research. 849. 187-195 (1999)

Y. Atsumi、K. Matsumoto 等人：“切除下牙槽神经后大鼠切牙牙周韧带中 Svhwann 细胞分布的改变：S-100 蛋白的免疫组织化学研究”大脑研究。

Y. Atsumi: "Altered distribution of Svhwann cells in the periodontal ligament of the rat incisor following resection of the inferior alveolar nerve : an immunohistochemical study on S-100 proteins"Brain Research. 849. 187-195 (1999)

Y. Atsumi：“下牙槽神经切除后大鼠切牙牙周韧带中 Svhwann 细胞分布的改变：S-100 蛋白的免疫组织化学研究”《大脑研究》。