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Role of neuropeptide on temporomandibular joint disorder

神经肽在颞下颌关节紊乱中的作用

基本信息

批准号：
08457549
负责人：
MATSUMOTO Ken
金额：
$ 3.9万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

In order to investigate the functional role of neuropeptides in primary afferent neurons in temporomandibular joint disorders, we carried out the following experiments.1) Topograohic localization of trigeminal neurons innervating the temporomandibular joint (TMJ) and relation to neuropeptides.Under anesthesia, TMJ was exposed unilaterally and small amount of 5% Fast Blue (FB) was injected into the joint space. Three days following injection, rats were perfused and the trigeminal ganglia were removed. Serial frozen sections were cut at a thickness of 20 mum and an indirect immunofluorescence method was applied to the sections.FB-labeled TMJ neurons were mostly localized at the border between the maxillary and mandibular regions of the ispilateral trigeminal ganglion. Some were distributed in the maxillary region. Most of them are medium-sized neurons. No FB-labeled neurons were observed in the contralateral trigeminal ganglion. Approximately 16.6% of FB-labeled TMJ neurons were immunoreactive for substance P (SP), and approximately 19.5% of them showed calcitonin generelated peptide (CGRP)-like immunoreactivity (-LI).2) Alterations in the levels of neuropeptides in the trigeminal ganglion following an experimental inflammation to the TMJ.An experimental inflammation of the TMJ was induced by injection of 2% complete Freund Adjuvant (CFA) to the joint space of the TMJ.Three days after injection of CFA,FB was applied, and an indirect immunofluorescence method was carried out. Approximately 12.6% of FB-labeled TMJ neurons displayd SP-LI,while approximately 17.9% of FB-labeled TMJ neurons expressed CGRP-LI following induction of the inflammation to the TMJ.P In conclusion, an experimental inflammation caused decreases in number of SP-like immunoreactive neurons in the trigeminal ganglion.

为了探讨神经肽在初级传入神经元在颞下颌关节疾病中的功能作用，我们进行了以下实验。1）支配颞下颌关节（TMJ）的三叉神经元的拓扑定位及其与神经肽的关系。麻醉下，单侧暴露TMJ，注射少量5%固蓝（FB）。关节空间。注射三天后，对大鼠进行灌注并去除三叉神经节。连续切片20μm厚，间接免疫荧光法检测。FB标记的TMJ神经元主要位于同侧三叉神经节上颌区和下颌区的交界处。有些分布在上颌区域。其中大多数是中等大小的神经元。在对侧三叉神经节中未观察到 FB 标记的神经元。大约 16.6% FB 标记的 TMJ 神经元对 P 物质 (SP) 具有免疫反应性，其中大约 19.5% 显示降钙素基因相关肽 (CGRP) 样免疫反应性 (-LI)。2) TMJ 实验性炎症后三叉神经节中神经肽水平的变化。通过向TMJ关节间隙注射2%完全弗氏佐剂(CFA)诱导TMJ。注射CFA后3天，应用FB，进行间接免疫荧光法。大约 12.6% 的 FB 标记的 TMJ 神经元显示 SP-LI，而大约 17.9% 的 FB 标记的 TMJ 神经元在诱导 TMJ 炎症后表达 CGRP-LI。总之，实验性炎症导致三叉神经节中 SP 样免疫反应性神经元数量减少。