Analysis of interactions of polymeric micelles with cells

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In the first year, a method to characterize drugs incorporated in polymeric micelles both in chemical conjugation and physical entrapment has been established. It was revealed that in the physical entrapment procedure, an adriamycin dimer formed. Furthermore, a method to incorporate desired amounts of adriamycin both in the chemical conjugation and the physical entrapment, individually. Based on these results, in vitro cytotoxic activity of the adriamycin-incorporated micelle against B16 mouse melanoma cells was evaluated. Then, distribution of adriamycin in this cell was observed by fluorescence microscopy. Polymeric micelles provided fainter adriamycin fluorescence image on nucleus than free adriamycin as compared in the same level of cytotoxic activity. This fact suggested that physically entrapped adriamycin, which was cytotoxically active, was invisible probably distributed in cytosol or lysosomes.