权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Identification and biosynthesis of sperm receptor in quail oocyte

鹌鹑卵母细胞精子受体的鉴定及生物合成

基本信息

批准号：
09660300
负责人：
MORI Makoto
金额：
$ 0.51万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660300/
关键词：
Vitelline membrane Sperm Receptor Fertilization Quail Fowl Follicle Cell division

项目摘要

The avian oocyte is bounded by the thin translucent membrane called vitelline membrane. The vitelline membrane consists of three layers, the inner and outer layers and the continuous membrane. Only the inner layer of vitelline membrane surrounds the oocyte at the time of ovulation. We have found that the granulosa cells of the preovulatory follicles contain 33 kDa glycoprotein (ZPC) one of the major components of the inner layer in quail egg. Though the physiological importance of ZPC for fertilization was pointed out as the candidate of "sperm receptor" in quail, the details in the regulation of ZPC production in granulosa cells during ovulation cycle remains obscure. To investigate the regulatory mechanism of ZPC production we have studied the expression of ZPC during the ovulation cycle by Western blot and Northern blot analyses. Moreover, we cultured the granulosa cells with or without various hormones and growth factors to study the endocrine and paracrine control of ZPC production. From the Western blot and Northern blot analyses in in-vivo study, the expression of ZPC in the granulosa cells of growing follicles increased during follicular maturation with the highest activity in the largest follicle. To investigate the effects of various hormones and growth factors on the ZPC production, the granulosa cells obtained from the three largest follicles were cultured for 72 hr with FSH, LH, EGF, IGF- I, IGF- II, TGF- alpha, TGF- beta, progesterone, estradiol, or testosterone. The most prominent effects on stimulation of ZPC production was observed in the granulosa cells isolated from the third largest follicle cultured by the addition of FSH.Other hormones or growth factors caused no significant increase. These data illustrate that quail granulosa cells produce ZPC in response to FSH.

禽类卵母细胞被称为卵黄膜的半透明薄膜所包围。卵黄膜由内层、外层和连续膜三层组成。排卵时，只有内层卵黄膜包围着卵母细胞。我们发现排卵前卵泡的颗粒细胞含有 33 kDa 糖蛋白 (ZPC)，它是鹌鹑蛋内层的主要成分之一。尽管 ZPC 对受精的生理重要性被指出是鹌鹑“精子受体”的候选者，但在排卵周期期间颗粒细胞中 ZPC 产生的调节细节仍然不清楚。为了研究 ZPC 产生的调节机制，我们通过 Western blot 和 Northern blot 分析研究了排卵周期中 ZPC 的表达。此外，我们培养了有或没有各种激素和生长因子的颗粒细胞，以研究 ZPC 生产的内分泌和旁分泌控制。体内研究的Western blot和Northern blot分析显示，生长中的卵泡颗粒细胞中ZPC的表达在卵泡成熟过程中增加，在最大的卵泡中活性最高。为了研究各种激素和生长因子对 ZPC 产生的影响，将从三个最大卵泡获得的颗粒细胞与 FSH、LH、EGF、IGF-I、IGF-II、TGF-α、TGF-β、孕酮、雌二醇或睾酮一起培养 72 小时。在通过添加FSH培养的第三大卵泡中分离的颗粒细胞中观察到对刺激ZPC产生最显着的影响。其他激素或生长因子没有引起显着的增加。这些数据说明鹌鹑颗粒细胞响应 FSH 产生 ZPC。