Histochemical study of the fine distribution and development of microcirculatory system.

微循环系统精细分布和发育的组织化学研究。

• 批准号: 09670020
• 负责人: KATO Seiji
• 金额: $ 2.05万
• 依托单位: Oita Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670020/
• 关键词: lymphatics; 5'-nucleotidase; microcirculation; histochemistry; blood vessel; alkaline phosphatase; development; diaminopeptidase; 5'-nucleotidase; Alkaline phosphatase; Diaminopeptidase; 循環

The microcirculatory system, lymphatics and blood vessels was histochemically studied in regards to the organ specificity of the structural organization and fine distribution of the lymphatic capillary network. The lymphatics and blood vessels are characterized by an enzyme-histochemical method using 5'-nucleotidase (5'-Nase), alkaline phosphatase (ALPase) and/or diaminopeptidase (DAPase) staining in addition to an immunohistochemical method. The 5'-Nase-positive lymphatic vessels can be distinguished histochemically from arterial and venous vessels based on ALPase and DAPase activity, respectively. The specificity and localization of enzyme reactions were confirmed by comparative histochemical studies of the same specimen under light microscope and scanning or transmission electron microscope. Furthermore, this report presents a new applicability of the backscattered electron imaginings of 5'-Nase reaction for lymphatics and acetyicholinesterase reactions for nerve plexus to scanning electron microscopic histochemistry stained with heavy metal, respectively. These histochemical methods are discussed in relation to their ability to demonstrate the organ specificity of vascular networks under normal and pathological condition.

组织化学研究了微循环系统、毛细血管和血管的结构组织和毛细淋巴管网络的精细分布的器官特异性。除了免疫组织化学方法外，还通过使用5 '-核苷酸酶（5'-Nase）、碱性磷酸酶（ALTH）和/或二氨基肽酶（DAPase）染色的酶组织化学方法表征血管和血管。5 '-Nase阳性淋巴管可分别根据ALTH和DAPase活性在组织化学上与动脉和静脉血管区分开来。通过在光学显微镜和扫描或透射电子显微镜下对同一标本进行比较组织化学研究，证实了酶反应的特异性和定位。此外，本文还提出了用5 '-Nase反应和乙酰胆碱酯酶反应的背散射电子显色法分别对重金属染色的扫描电镜组织化学进行应用的新方法。讨论了这些组织化学方法在正常和病理条件下证明血管网络器官特异性的能力。

项目成果

Shimoda,H.,Kato,S.,Kudo,T.& Usui,T.: "Lymphatic network and nerve plexus in the myerteric layer of the monkey jejunum:A topographic study using a enzyme-histockemical inethod" Arch.Histol.Cytol. 61. 65-73 (1998)

下田 H.、加藤 S.、工藤 T.

Shimoda, H., Kato, S.and Kudo, T.andUsui, T.: "Lymphatic network and nerve oplexus in the myenteric layer of the monkey jejunum : A topographic study using an enzyme-histochemicl method." Arch.Hitol.Cytol.61. 65-73 (1998)

Shimoda, H.、Kato, S.和 Kudo, T.andUsui, T.：“猴子空肠肌间层的淋巴网络和神经丛：使用酶组织化学方法的地形学研究。”

Miura,M.,Kats,S vcn Liiding hausen: "Lymphatic drainage of the cerebrospinal fluid from neonkey spinal meninger with special reference to the distributim of the epidual" Arch.Histol.Cytol.61. 277-286 (1998)

Miura,M.,Kats,S vcn Liiding hausen：“来自霓虹灯脊膜的脑脊液的淋巴引流，特别参考硬膜外膜的分布”Arch.Histol.Cytol.61。

Shimoda,H.,Kato,S.Kudo,T.: "Hists chemical demonstration of the intramural lymphotic networks in the digestive tract" Lymphology. 31(Suppl). 65-68 (1998)

Shimoda,H.,Kato,S.Kudo,T.：“消化道壁内淋巴网络的化学演示”淋巴学。

Ji,R.C.,Kato,S.,K Usui,T.: "Fine distribution and ultrastructure of lymphatic vessels in the NOD mice pancreas:An enzyme-histochemical study" Acta Anatomica Sinica. 29. 53-60 (1998)

Ji，R.C.，Kato，S.，K Usui，T.：“NOD小鼠胰腺中淋巴管的精细分布和超微结构：酶组织化学研究”解剖学报。