Where and how do the initial lymphatics develop?

初始淋巴管发育的酶组织化学研究。淋巴管的个体发生：研究了几个器官中初始淋巴管和毛细血管的精细结构和分布，特别关注了一些进食、呼吸等功能的发展。淋巴网络随着器官功能的发展而发展。淋巴管再生实验：对大鼠和猴皮肤损伤或移植后血管的酶组织化学性质进行了研究。这些皮肤组织的淋巴管和血管分别显示出高的5′-Nase和ALPase活性。肠内淋巴系统阻塞的影响：用光镜和电镜观察胸导管结扎术后淋巴血管的形态变化。TD结扎后，壁内和肠系膜淋巴管扩张，中央淋巴管5′-Nase活性降低。淋巴内皮细胞的体外增殖：采用胶原酶消化法从猴胸导管中分离出内皮细胞，在含内皮生长因子（EGF）的培养基中培养数周。培养的内皮细胞呈5′- nase阳性。需要进一步的检查来确定试管管的形成。

Enzyme-histochemical study of the development of initial lymphatics.1.Ontogeny of iymphatic vessels : Fine structure and distribution of initial lymphatics and blood capillaries in several organs were studied with special reference to the the development of some functions of feeding, respiration et al. The lymphatic network developed according to the development of the function of the organs.2.Experiment of the regeneration of lymphatics : Enzyme-histochemical property of the vessels in the skins, which were injured or transplanted, were examined in the rat and monkey. The lymphatics and blood vessels in these skin tissues revelaed high 5'-Nase and ALPase activity respectively.3.The effect of the blockage of the lymphatic system in the intestne : The morphological changes of lymphatic vessels after thoracic duct (TD) ligation were examined using a light and electron microscope. After the TD ligation, intramural and mesenteric lymphatic vessels were distended and 5'-Nase activity of the central lymphatic vessels decreased.4.in vitro proliferation of lymphatic endotherial cells : The endotherial cells were separated from the monkey thoracic duct using collagenase digestive method and cultured in the medium with endotherial growth factor (EGF) for a few weeks. The cultured enthotheral cells were 5'-Nase-positive. Further examination is needed to ascertain the canal formation in vitro.