Where and how do the initial lymphatics develop?

最初的淋巴管在哪里以及如何发育？

• 批准号: 05670019
• 负责人: KATO Seiji
• 金额: $ 1.47万
• 依托单位: Oita Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05670019/
• 关键词: Lymphatics; 5'-nucleotidase; Microcirculation; Enzyme histochemistry; TEM; Alkaline phosphatase; SEM; Blood vessel; Alkaline phosphatase; 5'-nucleatidase; Alkaline Phosphatase

Enzyme-histochemical study of the development of initial lymphatics.1.Ontogeny of iymphatic vessels : Fine structure and distribution of initial lymphatics and blood capillaries in several organs were studied with special reference to the the development of some functions of feeding, respiration et al. The lymphatic network developed according to the development of the function of the organs.2.Experiment of the regeneration of lymphatics : Enzyme-histochemical property of the vessels in the skins, which were injured or transplanted, were examined in the rat and monkey. The lymphatics and blood vessels in these skin tissues revelaed high 5'-Nase and ALPase activity respectively.3.The effect of the blockage of the lymphatic system in the intestne : The morphological changes of lymphatic vessels after thoracic duct (TD) ligation were examined using a light and electron microscope. After the TD ligation, intramural and mesenteric lymphatic vessels were distended and 5'-Nase activity of the central lymphatic vessels decreased.4.in vitro proliferation of lymphatic endotherial cells : The endotherial cells were separated from the monkey thoracic duct using collagenase digestive method and cultured in the medium with endotherial growth factor (EGF) for a few weeks. The cultured enthotheral cells were 5'-Nase-positive. Further examination is needed to ascertain the canal formation in vitro.

1.淋巴管的发生：研究了淋巴管和毛细血管在几种器官中的微细结构和分布，特别是对摄食、呼吸等某些功能的发育进行了研究。淋巴管网是随着器官功能的发育而发育的。2.淋巴管再生实验：本文观察了大鼠和猴皮肤损伤和移植后血管的酶组织化学性质。3.阻断淋巴系统对大鼠胸导管结扎后淋巴管的影响：光镜和电镜观察胸导管结扎后淋巴管的形态学变化。TD结扎后，扩张壁内和肠系膜淋巴管，并测定中央淋巴管的5 ′-Nase活性。淋巴管内皮细胞的体外增殖：用胶原酶消化法从猴胸导管分离内皮细胞，并在含有内皮生长因子（EGF）的培养基中培养数周。decreased.4.in培养的端栖动物细胞呈5 '-Nase阳性。需要进一步的检查来确定在体外的管道形成。

项目成果

Fujiwara, T., Kato, S., Itonaga, I.et al.: "Fine structure and distribution of lymphatics in the synovial membrane of monkey and human knee joints" International Orthopeadics (S/COT). 19. 396-402 (1995)

Fujiwara, T.、Kato, S.、Itonaga, I.等人：“猴子和人类膝关节滑液膜中淋巴管的精细结构和分布”国际骨科 (S/COT)。

加藤征治・三浦真弘・糸永 門: "血管(静脈・動脈)とリンパ管の鑑別のための三重染色法" リンパ学. 17. 1-6 (1994)

Seiji Kato、Masahiro Miura 和 Mon Itonaga：“用于区分血管（静脉和动脉）和淋巴管的三重染色方法”Lymphology。17. 1-6 (1994)。

加藤征治: "組織学-組織化学的アプローチ分担第5章脈管" 朝倉書店,東京, 37-43 (1996)

Seiji Kato：“组织学-组织化学方法第 5 章脉管系统”朝仓书店，东京，37-43 (1996)

加藤征治・三浦真弘・中村英次郎・宮内亮輔: "漿膜下組織内リンパ管網の酵素組織化学的抽出-伸展試料の二重染色" リンパ学. 16(1). 19-26 (1993)

Seiji Kato、Masahiro Miura、Eijiro Nakamura、Ryosuke Miyauchi：“浆膜下组织中淋巴网络的酶组织化学提取 - 拉伸标本的双重染色”16(1) (1993)。

Kato, S., Itonaga, I., Ji, R.C.& Miura, M.: "Engyme triple for differentiation of lymphaties from staining venous and arterial capillaries" Lymphology. 29 (in press). (1996)

加藤，S.，伊东永，I.，吉，R.C.