Histochemical study on the specific antibody for lymphatic endothelial cells and endothelial growth factors

组织化学研究了微循环系统、毛细血管和血管的结构组织和毛细淋巴管网络的精细分布的器官特异性。除了免疫组织化学方法外，还通过使用5 '-核苷酸酶（5'-Nase）、碱性磷酸酶（ALTH）和/或二氨基肽酶（DAPase）染色的酶组织化学方法表征血管和血管。5 '-Nase阳性淋巴管可分别根据ALTH和DAPase活性与动脉和静脉血管进行组织化学区分。酶反应的特异性和定位通过同一标本在光镜和扫描或透射电镜下的比较组织化学研究来证实。此外，本报告还介绍了我们实验室研制的一种新的5 '-Nase单克隆抗体。讨论了这些酶和免疫组织化学方法在正常和病理条件下证明血管网络发育的器官特异性的能力。

The microcirculatory system, lymphatics and blood vessels was histochemically studied in regards to the organ specificity of the structural organization and fine distribution of the lymphatic capillary network. The lymphatics and blood vessels are characterized by an enzyme-histochemical method using 5'-nucleotidase (5'-Nase), alkaline phosphatase (ALPase) and / or diaminopeptidase (DAPase) staining in addition to an immunohistochemical method. The 5'-Nase-positive lymphatic vessels could be distinguished histochemically from arterial and venous vessels based on ALPase and DAPase activity, respectively. The specificity and localization of enzyme reactions were confirmed by comparative histochemical studies of the same specimen under light microscope and scanning or transmission electron microscope. Furthermore, this report presents a new monoclonal antibody for 5'-Nase which we developed in our laboratory. These enzyme-and immunohistochemical methods are discussed in relation to their ability to demonstrate the organ specificity of developing vascular networks under normal and pathological condition.