Histochemical study on the specific antibody for lymphatic endothelial cells and endothelial growth factors

淋巴管内皮细胞及内皮生长因子特异性抗体的组织化学研究

• 批准号: 12670021
• 负责人: KATO Seiji
• 金额: $ 0.64万
• 依托单位: OITA MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670021/
• 关键词: lymphatics; 5'-nucleotidase; microcirculation; histochemistry; blood vessel; endothelial growth factor; monoclonal antibody; diaminopeptidase

The microcirculatory system, lymphatics and blood vessels was histochemically studied in regards to the organ specificity of the structural organization and fine distribution of the lymphatic capillary network. The lymphatics and blood vessels are characterized by an enzyme-histochemical method using 5'-nucleotidase (5'-Nase), alkaline phosphatase (ALPase) and / or diaminopeptidase (DAPase) staining in addition to an immunohistochemical method. The 5'-Nase-positive lymphatic vessels could be distinguished histochemically from arterial and venous vessels based on ALPase and DAPase activity, respectively. The specificity and localization of enzyme reactions were confirmed by comparative histochemical studies of the same specimen under light microscope and scanning or transmission electron microscope. Furthermore, this report presents a new monoclonal antibody for 5'-Nase which we developed in our laboratory. These enzyme-and immunohistochemical methods are discussed in relation to their ability to demonstrate the organ specificity of developing vascular networks under normal and pathological condition.

组织化学研究了微循环系统、毛细血管和血管的结构组织和毛细淋巴管网络的精细分布的器官特异性。除了免疫组织化学方法外，还通过使用5 '-核苷酸酶（5'-Nase）、碱性磷酸酶（ALTH）和/或二氨基肽酶（DAPase）染色的酶组织化学方法表征血管和血管。5 '-Nase阳性淋巴管可分别根据ALTH和DAPase活性与动脉和静脉血管进行组织化学区分。酶反应的特异性和定位通过同一标本在光镜和扫描或透射电镜下的比较组织化学研究来证实。此外，本报告还介绍了我们实验室研制的一种新的5 '-Nase单克隆抗体。这些酶和免疫组化方法进行了讨论，在正常和病理条件下，他们的能力，以证明器官特异性的发展血管网络。

项目成果

Shimoda H, Kudo T, Takahashi Y: "Ultrastructural and histochemical characterization of special muscle cells in the monkey small intestine"Arch.Histol.Cytol.. 63. 217-228 (2000)

Shimoda H、Kudo T、Takahashi Y：“猴小肠特殊肌肉细胞的超微结构和组织化学特征”Arch.Histol.Cytol.. 63. 217-228 (2000)

Shimoda H., Takahashi Y., Kato S: "Development of the lymphatic network in the muscle coat of the rat jejunum as revealed by enzyme-histochemistry"Arch.Histol.Cytol.. 64. 523-533 (2001)

Shimoda H.、Takahashi Y.、Kato S：“通过酶组织化学揭示大鼠空肠肌层中淋巴网络的发育”Arch.Histol.Cytol.. 64. 523-533 (2001)

Yoshioka K,Nishimaru N Shimoda H,Yamada K: "Characteristics of monocarboxylates as energy substrates other than glucose in rat brain slices and the effect of selective glial poisoning-A^<31> P NMR study-"Neuroscience Research. 36. 215-226 (2000)

Yoshioka K，Nishimaru N Shimoda H，Yamada K：“大鼠脑切片中除葡萄糖外的单羧酸盐作为能量底物的特征以及选择性神经胶质中毒的影响-A^<31> P NMR研究-”神经科学研究。

H Shimoda, Y Takahashi, S Kato: "Development of the Lymphatic Network in the Muscle Coat of the Rat Jejunum as Revealed by Enzyme-Histochemistry"Arch.Histol.Cytol.. 64(5). 523-533 (2001)

H Shimoda、Y Takahashi、S Kato：“酶组织化学揭示的大鼠空肠肌肉层淋巴网络的发育”Arch.Histol.Cytol.. 64(5)。

三浦真弘,加藤征治: "腹膜・間膜のリンパ管系の分布とその構築-組織科学的観察-"リンパ学. 23巻1号. 23-32 (2000)

Masahiro Miura，Seiji Kato：“腹膜和肠系膜淋巴系统的分布和构建 - 组织学观察”，第 23 卷，第 1. 23-32 期（2000 年）。