Analysis of the specific molecule expression in lymphangiogenesis and cancer metastsis

淋巴管生成和癌症转移中特异性分子表达分析

• 批准号: 16590147
• 负责人: KATO Seiji
• 金额: $ 2.05万
• 依托单位: Oita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590147/
• 关键词: Lymphatic vessel; Lymphangiogenesis; Cancer metastasis; Vascular endothelial growth factor; Endothelial specific marker; Histochemistry; Podoplanin; VEGF-C; 酵素組織化学; 免疫組織化学

The recent development and application of new histochemical methods for identifying lymphatics using endothelial specific markers have enabled the distinction between lymphatic and blood vessels at the capillary level, resulting in marked advance in the study of lymphatics. In particular, several functional molecules specifically expressed and localized in lymphatic endothelial cells, such as 5'-nucleotidase, lymphatic endothelial growth factor receptor-1 tyrosine kinase, vascular endothelial growth factor receptor-3, podoplanin and Prox-1, have been identified. The biology of lymphangiogenesis, particularly the mechanism of its regulation, is very important in understanding the formation of the lymphatic system as a biological regulation system transporting tissue fluid and wandering cells, including lymphocytres, and disease involving lymphangiogenesis. The understanding of the molecular mechanism of lymphangiogenesis and the elucidation of the development of normal and pathological tissues are expected to lead to the development of therapy for intractable diseases, such as malignant tumors and lymphedema. We examined the histochemical insight into the field of lymphangiogenesis, with special emphasis on the novel and reliable lymphatic endothelial cell markers, as well as on lymphatic regeneration after experimental injury.

近年来，内皮特异性标记物用于鉴别淋巴管的组织化学新方法的发展和应用，使淋巴管和血管在毛细血管水平上得以区分，从而使淋巴管的研究取得了显著进展。特别是，已经鉴定了几种特异性表达和定位于淋巴管内皮细胞中的功能分子，例如5 '-核苷酸酶、淋巴管内皮生长因子受体-1酪氨酸激酶、血管内皮生长因子受体-3、podoplanin和Prox-1。淋巴管生成的生物学，特别是其调节机制，对于理解淋巴系统作为运输组织液和游走细胞（包括淋巴细胞）的生物调节系统的形成以及涉及淋巴管生成的疾病是非常重要的。对淋巴管生成的分子机制的理解以及对正常和病理组织的发展的阐明有望导致对诸如恶性肿瘤和水肿等难治性疾病的治疗的发展。我们研究了淋巴管生成领域的组织化学见解，特别强调了新的和可靠的淋巴管内皮细胞标记物，以及实验性损伤后的淋巴再生。

项目成果

Functional morphology of lymphatic vessels in the gut as revealed by histochemical methods

组织化学方法揭示肠道淋巴管的功能形态

• 发表时间: 2004
• 期刊: Lymphology(Suppl) 37
• 作者: Shimoda H;Kato S
• 通讯作者: Kato S

Study on pancreatic lymphatics in nonobese diabetic mouse with inhibition of insulitis and diabetes by adjuvant immunotherapy.

非肥胖糖尿病小鼠胰腺淋巴管通过辅助免疫疗法抑制胰岛素炎和糖尿病的研究。

• 发表时间: 2004
• 期刊: Anat. Rec. PartA 281A
• 作者: Qu P;Ji RC;Shimoda H;Miura M;Kato S
• 通讯作者: Kato S

腸管組織再生におけるリンパ管新生

肠组织再生中的淋巴管生成

• 发表时间: 2004
• 期刊: リンパ学 27(2)
• 作者: 下田 浩;梶原 徹;加藤征冶
• 通讯作者: 加藤征冶

Expression of VEGFR-3 and 5′-nase in regenerating lymphatic vessels of the cutaneous wound healing

• DOI: 10.1002/jemt.20082
• 发表时间: 2004-06-15
• 期刊: MICROSCOPY RESEARCH AND TECHNIQUE
• 影响因子: 2.5
• 作者: Ji, RC;Miura, M;Kato, S
• 通讯作者: Kato, S

Effect of sucralfate on antibiotic therapy for helicobacter pylori infection in mice.

硫糖铝对小鼠幽门螺杆菌感染抗生素治疗的影响。

• 发表时间: 2004
• 期刊: Antimicrobial Agents and Chemotherapy 48
• 作者: Watanabe K;Murakami K;Sato R;Miura M;Ootsu S;Miyajima H;Nasu M;Okimoto M;Kodama M;Fujioka T
• 通讯作者: Fujioka T