Analysis of the specific molecule expression in lymphangiogenesis and cancer metastsis

近年来，内皮特异性标记物用于鉴别淋巴管的组织化学新方法的发展和应用，使淋巴管和血管在毛细血管水平上得以区分，从而使淋巴管的研究取得了显著进展。特别是，已经鉴定了几种特异性表达和定位于淋巴管内皮细胞中的功能分子，例如5 '-核苷酸酶、淋巴管内皮生长因子受体-1酪氨酸激酶、血管内皮生长因子受体-3、podoplanin和Prox-1。淋巴管生成的生物学，特别是其调节机制，对于理解淋巴系统作为运输组织液和游走细胞（包括淋巴细胞）的生物调节系统的形成以及涉及淋巴管生成的疾病是非常重要的。对淋巴管生成的分子机制的理解以及对正常和病理组织的发展的阐明有望导致对诸如恶性肿瘤和水肿等难治性疾病的治疗的发展。我们研究了淋巴管生成领域的组织化学见解，特别强调了新的和可靠的淋巴管内皮细胞标记物，以及实验性损伤后的淋巴再生。

The recent development and application of new histochemical methods for identifying lymphatics using endothelial specific markers have enabled the distinction between lymphatic and blood vessels at the capillary level, resulting in marked advance in the study of lymphatics. In particular, several functional molecules specifically expressed and localized in lymphatic endothelial cells, such as 5'-nucleotidase, lymphatic endothelial growth factor receptor-1 tyrosine kinase, vascular endothelial growth factor receptor-3, podoplanin and Prox-1, have been identified. The biology of lymphangiogenesis, particularly the mechanism of its regulation, is very important in understanding the formation of the lymphatic system as a biological regulation system transporting tissue fluid and wandering cells, including lymphocytres, and disease involving lymphangiogenesis. The understanding of the molecular mechanism of lymphangiogenesis and the elucidation of the development of normal and pathological tissues are expected to lead to the development of therapy for intractable diseases, such as malignant tumors and lymphedema. We examined the histochemical insight into the field of lymphangiogenesis, with special emphasis on the novel and reliable lymphatic endothelial cell markers, as well as on lymphatic regeneration after experimental injury.