RADIOSENSITIVITY AND APOPTOSIS OF SPERMATOGONIA
精原细胞的放射敏感性和细胞凋亡
基本信息
- 批准号:09670917
- 负责人:
- 金额:$ 0.32万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The purpose of this study is to identify the mode of radiation-induced cell death in the tesitis and other tissue.Male C57BL or BALB/cA Jcl-nu mice were irradiated with single doses of 0.5, 2.5 or 5.0 Gy to investigate the morphological features of radiation-induced cell death. Male p53 knockout mice, homozygously null p53(-/-) and heterozygous p53(+I-) mice (CBA/OYC), and wild type mice (CBA/OYC) were irradiated with a single dose of 5.0 Gy to determine whether the radiation- induced cell death was p53-dependent.The radiation-induced cell death of spermatogonia reached a peak at 12 hours after irradiation, but that of other tissue was at 6 hours after irradiation. Nuclei of many abnormal spermatogonia were condensed and TUNEL-positive, though margination of nuclear chromatin and nuclear fragmentation was rare. The incidence of radiation-induced cell death in testis was markedly increased in p53(+I+) mice, but there was little change in p53(-/-) mice. Apoptosis of lymphocytes and fibrosarcoma cells inceased markedly following irradiation in the p53(+/+) or p53(+/-) genotype, but that increased just a little in the p53(-/-) genotype.Spermatogonial cell death induced by irradiation is compatible with p53-dependent apoptosis, though the morphological features are not always typical.
为探讨放射诱导的结缔组织炎和其他组织细胞死亡的方式,采用0.5、2.5和5.0戈伊单次照射雄性C57 BL或BALB/cA Jcl-nu小鼠,观察放射诱导细胞死亡的形态学特征。用5.0戈伊单次照射雄性p53基因敲除小鼠、p53(-/-)和杂合型p53(+I-)小鼠(CBA/OYC)及野生型小鼠(CBA/OYC),观察照射后精原细胞的死亡是否为p53依赖性。而其他组织在照射后6 h才出现。许多异常精原细胞的细胞核浓缩和TUNEL阳性,虽然边集核染色质和核碎裂是罕见的。p53(+I+)小鼠睾丸放射性细胞死亡率明显增加,而p53(-/-)小鼠睾丸放射性细胞死亡率无明显变化。照射后p53(+/+)和p53(+/-)基因型的淋巴细胞和纤维肉瘤细胞凋亡明显增加,而p53(-/-)基因型的细胞凋亡增加不明显。
项目成果
期刊论文数量(20)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hasegawa M,Mitsuhashi N,Yamakawa M,Furuta M,Maebayashi K,Imai R,Hayakawa K,Niibe H.: "p53 protein expression and radiation-induced apoptosis in human tumors transplanted to nude mice" Radiat Med. 15. 171-176 (1997)
Hasekawa M、Mitsuhashi N、Yamakawa M、Furuta M、Maebayashi K、Imai R、Hayakawa K、Niibe H.:“移植到裸鼠的人类肿瘤中的 p53 蛋白表达和辐射诱导的细胞凋亡”Radiat Med。
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- 影响因子:0
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Kazushige Hayakawa: "The prognostic significance of immunohistochemically detected p53 protein expression in non-small cell lung cancer treated with radiation therapy" Anticancer Res.18. 3685-3688 (1998)
Kazushige Hayakawa:“免疫组织化学检测 p53 蛋白表达在放射治疗的非小细胞肺癌中的预后意义”Anticancer Res.18。
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- 影响因子:0
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Hideyuki Sakurai: "Early radiation effects in highly apoptotic murine lymphoma xenografts monitered by 31P magnetic resonance spectroscopy" Int.J.Radiat.Oncol.Biol.Phys.41. 1157-1162 (1998)
Hideyuki Sakurai:“通过 31P 磁共振波谱监测高度凋亡小鼠淋巴瘤异种移植物的早期辐射效应”Int.J.Radiat.Oncol.Biol.Phys.41。
- DOI:
- 发表时间:
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- 影响因子:0
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- 通讯作者:
Masatoshi Hasegawa: "Resistance of differentiating spermatogonia to radiation-induced apoptosis and loss in p53-deficient mice" Radiat.Res.149. 263-270 (1998)
Masatoshi Hasekawa:“p53 缺陷小鼠中分化精原细胞对辐射诱导的细胞凋亡和损失的抵抗”Radiat.Res.149。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Masatoshi Hasegawa: "P53 protein expression and radiation-induced apoptosis in human tumors transplanted to nude mice" Rediation Medicine. 15・3. 171-176 (1997)
Masatoshi Hasekawa:“移植到裸鼠的人类肿瘤中的 P53 蛋白表达和辐射诱导的细胞凋亡”Rediation Medicine 15・3(1997)。
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- 影响因子:0
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HASEGAWA Masatoshi其他文献
Kanjin (urging people to do a meritorious act) and The relief of the people of nenbutu-hijiri (holy men) in the Tsugaru region
劝人与津轻地区念佛圣人的救济
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- 发表时间:
2005 - 期刊:
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SHIMIZU Tetsuro;SHIMIZU Tetsuro;山崎 裕子;YAMAZAKI Hiroko;SHIMIZU Tetsuro;山崎 裕子;樋笠 勝士;樋笠 勝士;樋笠 勝士;IWAKUMA Yukio;IWAKUMA Yukio;IWAKUMA Yukio;岩熊 幸男;山内 志朗;加藤 守通;神崎 繁;HIKASA Katsushi;HIKASA Katsushi;HIKASA Katsushi;IWAKUMA Yukio;末木 文美士;圭室文雄;IWAKUMA Yukio;IWAKUMA Yukio;長谷川 匡俊;IWAKUMA Yukio;SUEKI Fumihiko;YAMAUCHI Shiro;TAMAMURO Fumio;Kanzaki Shigeru;HASEGAWA Masatoshi - 通讯作者:
HASEGAWA Masatoshi
『東日流外三群誌』問題雑感
关于东一龙街三军师的其他问题
- DOI:
- 发表时间:
2005 - 期刊:
- 影响因子:0
- 作者:
SHIMIZU Tetsuro;SHIMIZU Tetsuro;山崎 裕子;YAMAZAKI Hiroko;SHIMIZU Tetsuro;山崎 裕子;樋笠 勝士;樋笠 勝士;樋笠 勝士;IWAKUMA Yukio;IWAKUMA Yukio;IWAKUMA Yukio;岩熊 幸男;山内 志朗;加藤 守通;神崎 繁;HIKASA Katsushi;HIKASA Katsushi;HIKASA Katsushi;IWAKUMA Yukio;末木 文美士;圭室文雄;IWAKUMA Yukio;IWAKUMA Yukio;長谷川 匡俊;IWAKUMA Yukio;SUEKI Fumihiko;YAMAUCHI Shiro;TAMAMURO Fumio;Kanzaki Shigeru;HASEGAWA Masatoshi;IWAKUMA Yukio;末木文美士 - 通讯作者:
末木文美士
總持寺香資権化一件について
关于总持寺香火权取得案
- DOI:
- 发表时间:
2005 - 期刊:
- 影响因子:0
- 作者:
SHIMIZU Tetsuro;SHIMIZU Tetsuro;山崎 裕子;YAMAZAKI Hiroko;SHIMIZU Tetsuro;山崎 裕子;樋笠 勝士;樋笠 勝士;樋笠 勝士;IWAKUMA Yukio;IWAKUMA Yukio;IWAKUMA Yukio;岩熊 幸男;山内 志朗;加藤 守通;神崎 繁;HIKASA Katsushi;HIKASA Katsushi;HIKASA Katsushi;IWAKUMA Yukio;末木 文美士;圭室文雄;IWAKUMA Yukio;IWAKUMA Yukio;長谷川 匡俊;IWAKUMA Yukio;SUEKI Fumihiko;YAMAUCHI Shiro;TAMAMURO Fumio;Kanzaki Shigeru;HASEGAWA Masatoshi;IWAKUMA Yukio;末木文美士;圭室文雄 - 通讯作者:
圭室文雄
HASEGAWA Masatoshi的其他文献
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{{ truncateString('HASEGAWA Masatoshi', 18)}}的其他基金
Effect of heavy ion beam irradiation on radioresistance and differentiation of cancer stem cells in neuronal tumors
重离子束照射对神经元肿瘤干细胞放射抗性和分化的影响
- 批准号:
23390304 - 财政年份:2011
- 资助金额:
$ 0.32万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Novel High Frequency-compatible Low-CTE and Low-CHE Heat-resistant Dielectric Materials
新型高频兼容低 CTE 和低 CHE 耐热介电材料
- 批准号:
21550212 - 财政年份:2009
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$ 0.32万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular biological analysis of intratumoral inhomogeneous radiosensitivity
瘤内不均匀放射敏感性的分子生物学分析
- 批准号:
19390324 - 财政年份:2007
- 资助金额:
$ 0.32万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
p53-independent apoptosis, non-apoptotic programmed cell death, and organelle
不依赖 p53 的细胞凋亡、非凋亡性程序性细胞死亡和细胞器
- 批准号:
17591246 - 财政年份:2005
- 资助金额:
$ 0.32万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of High Temperature Polymers Possessing Low Dielectric Constant, Low Coefficient of Thermal Expansion, and High Transparency and Their Application to Electronic Devices.
低介电常数、低热膨胀系数、高透明性高温聚合物的开发及其在电子器件中的应用。
- 批准号:
16550179 - 财政年份:2004
- 资助金额:
$ 0.32万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Radiation-induced p53-independent apoptosis and radiosensitivity in human tumors
人类肿瘤中放射诱导的不依赖于 p53 的细胞凋亡和放射敏感性
- 批准号:
15591257 - 财政年份:2003
- 资助金额:
$ 0.32万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The synthetic research of the social welfare formation history in the area
地区社会福利形成历史综合研究
- 批准号:
15330119 - 财政年份:2003
- 资助金额:
$ 0.32万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The general study on the history and the present situation of social welfare by buddhist groups after WW II.
二战后佛教团体社会福利的历史与现状综述
- 批准号:
12410058 - 财政年份:2000
- 资助金额:
$ 0.32万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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