Effect of heavy ion beam irradiation on radioresistance and differentiation of cancer stem cells in neuronal tumors

重离子束照射对神经元肿瘤干细胞放射抗性和分化的影响

• 批准号: 23390304
• 负责人: HASEGAWA Masatoshi
• 金额: $ 12.56万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390304/
• 关键词: 癌幹細胞; 神経幹細胞; 放射線抵抗性; アポトーシス; p53; 炭素イオン線; Ki-67; CD133; cDNAアレイ解析; 膠芽腫

The purpose is to investigate the radiobiological effect of heavy ion (CI) beams on radioresistance and differentiation of human tumor cells, especially cancer stem cells. Human ependymoblastoma with wild-type p53 and glioblastoma with mutant-type p53 were transplanted into nude mice and irradiated with CI beams or 150-kV X-rays. Tumors were excised for cDNA microarray analysis 6- 48 hours or 6 weeks after 2-24 Gy irradiation. CD133, CD44, Nestin, p53, PTEN, Ki67, and GFAP expressions were evaluated immunohistochemically. In ependymoblastoma, p53 signaling and apoptosis-related pathways were suggested to be involved following irradiation. The tumors showed a significant decrease in growth fraction and a slight increase of the CD133-positive rate after high-dose irradiation. These results suggest that CD133-positive and Ki-67-negative cells may be resistant to both X-rays and CI beams.

目的是研究重离子（CI）束对人类肿瘤细胞，特别是癌症干细胞的放射抗性和分化的放射生物学作用。将野生型p53的人室管膜母细胞瘤和突变型p53的胶质母细胞瘤移植到裸鼠体内，并用CI束或150-kV X射线照射。 2-24Gy辐射后6-48小时或6周切除肿瘤用于cDNA微阵列分析。通过免疫组织化学方法评估 CD133、CD44、Nestin、p53、PTEN、Ki67 和 GFAP 表达。在室管膜母细胞瘤中，p53 信号传导和细胞凋亡相关途径被认为在辐射后参与其中。高剂量照射后肿瘤生长分数显着降低，CD133阳性率略有增加。这些结果表明 CD133 阳性和 Ki-67 阴性细胞可能对 X 射线和 CI 束具有抵抗力。

项目成果

Changes of Gene Expression Profiles and Cancer Stem Cell Markers in Brain Tumors Following Carbon Ion Beam Irradiation

碳离子束照射后脑肿瘤基因表达谱和癌症干细胞标志物的变化

• 发表时间: 2013
• 作者: Hasegawa M ;et al
• 通讯作者: et al

Cancer Stem Cell Markers and Gene Expression Profiles in Human Brain Tumors Following Carbon Ion Beam Irradiation

碳离子束照射后人脑肿瘤中的癌症干细胞标志物和基因表达谱

• 发表时间: 2013
• 作者: Hasegawa M;et al
• 通讯作者: et al

放射線抵抗性の p53 変異型膠芽腫における炭素線照射による遺伝子発現の検討

碳离子辐照诱导抗辐射 p53 突变胶质母细胞瘤基因表达的检测

• 发表时间: 2012
• 作者: 長谷川正俊;他
• 通讯作者: 他

粒子線治療の放射線生物学

粒子束治疗的放射生物学

• 发表时间: 2013
• 作者: 長谷川正俊 ;他
• 通讯作者: 他

Pathological and clinical impact of neoadjuvant chemoradiotherapy using full-dose gemcitabine and concurrent radiation for resectable pancreatic cancer

• DOI: 10.1007/s00534-012-0532-8
• 发表时间: 2013-02-01
• 期刊: JOURNAL OF HEPATO-BILIARY-PANCREATIC SCIENCES
• 影响因子: 3
• 作者: Sho, Masayuki;Akahori, Takahiro;Nakajima, Yoshiyuki
• 通讯作者: Nakajima, Yoshiyuki