p53-independent apoptosis, non-apoptotic programmed cell death, and organelle

不依赖 p53 的细胞凋亡、非凋亡性程序性细胞死亡和细胞器

• 批准号: 17591246
• 负责人: HASEGAWA Masatoshi
• 金额: $ 2.3万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591246/
• 关键词: p53; apoptosis; cell death; organella; radiation; glioblastoma; PIXE; 生物効果比

(1) We subcutaneously transplanted cells of human ependymoblastoma, glioblastoma, and small cell lung cancer (SCLC) into nude mice, irradiated them with 10 Gy X-ray at 200 kV, excised the tumors 3, 4.5, or 6 hours after irradiation, produced sections, and examined the changes in the distribution of elements by cell death, apoptosis and necrosis, using an in-air micro-particle-induced X-ray emission (micro-PIXE). In the non-irradiated tumors, phosphorus (P), potassium (K), chlorine (C1), and sulfur (S) were observed in the nuclei and cytoplasm of viable tumor cells, and these elements were significantly decreased in small necrotic lesions. In ependymoblastoma, apoptosis was markedly induced after irradiation, but it was difficult to clearly detect the changes in the distribution of these elements by the micro-PIXE. In glioblastoma and SCLC, small necrotic lesions were observed, and P, K, Cl, and S were significantly decreased, agreeing well with morphological findings.(2) Human brain tu … More mors (glioblastoma, ependymoblastoma, and primitive neuroectodermal tumor : PNET) were transplanted to nude mice subcutaneously, and the mice were irradiated with carbon ion beams (290MeV/u, 6cm SOBP) or 200kV X-rays. Tumors, brains, spleens, kidneys, duodenums, and testes were excised and weighed 6 or 48 hours after irradiation, and they were microscopically examined to evaluate apoptosis and other histological changes. Carbon ion beams were more effective than X-rays and the RBE values were more than 1.0, but the values varied according to the tumors and endpoints. The incidences of apoptosis 6 hours after 0.25-1 Gy irradiation were 5-49/high power field (HPF) by carbon ion and 3-9/HPF by X-ray in the ependymoblastoma. Those values were 1.2-4.0/HPF by carbon ion and 0.4-1.0/HPF by X-ray in the glioblastoma. RBE values were 3.0, 3.6, and 6.1 for the ependymoblastoma, the PNET, and the glioblastoma, respectively. In contrast, RBE values of radiosensitive normal tissues were 1.2-2.1. These results suggest that the heavy ion beams are more favorable for treating radioresistant tumors than X-rays. Less

(1)我们将人室管膜母细胞瘤、胶质母细胞瘤和小细胞肺癌（SCLC）细胞皮下移植到裸鼠体内，用200 kV的10戈伊X射线照射，照射后3、4.5或6小时切除肿瘤，制作切片，通过细胞死亡、凋亡和坏死检查元素分布的变化，使用空气中微粒诱导的X射线发射（micro-PIXE）。在未照射的肿瘤中，在存活肿瘤细胞的细胞核和细胞质中观察到磷（P）、钾（K）、氯（C1）和硫（S），并且这些元素在小的坏死病变中显著减少。在室管膜母细胞瘤中，照射后明显诱导细胞凋亡，但用micro-PIXE很难清楚地检测到这些元素分布的变化。在胶质母细胞瘤和小细胞肺癌中，观察到小的坏死病变，P、K、Cl和S显著降低，与形态学结果一致。(2)人脑图 ...更多信息 将胶质母细胞瘤、室管膜母细胞瘤和原始神经外胚层瘤（PNET）移植到裸鼠皮下，并用碳离子束（290 MeV/u，6cm SOBP）或200 kV X射线照射。在照射后6或48小时，切除肿瘤、脑、脾、肾、睾丸和睾丸并称重，并对其进行显微镜检查以评价细胞凋亡和其他组织学变化。碳离子束比X射线更有效，RBE值大于1.0，但该值因肿瘤和终点而异。室管膜母细胞瘤0.25 ~ 1戈伊照射后6小时，细胞凋亡率为5-49/高倍视野（HPF），X线为3-9/HPF。在胶质母细胞瘤中，碳离子的放射性活度为1.2-4.0/HPF，X线的放射性活度为0.4-1.0/HPF。室管膜母细胞瘤、PNET和胶质母细胞瘤的RBE值分别为3.0、3.6和6.1。而放射敏感的正常组织的RBE值为1.2-2.1。这些结果表明，重离子束比X射线更有利于治疗放射抗性肿瘤。少

项目成果

Cytotoxic effect of high linear energy transfer charged particle radiation on human glioblastoma cells

高线性能量转移带电粒子辐射对人胶质母细胞瘤细胞的细胞毒性作用

• 发表时间: 2006
• 期刊: JAEA-Review 2005-1
• 作者: Ishiuchi S;Hasegawa M;Kobayashi;et al.
• 通讯作者: et al.

放射線治療の進歩

放射治疗的进展

• 发表时间: 2007
• 期刊: 奈良医学雑誌 58・1
• 作者: 長谷川正俊;他
• 通讯作者: 他

Analysis of the distribution and the dynamics of trace elements associated with radiation- and drug-induced apoptosis

与辐射和药物诱导的细胞凋亡相关的微量元素的分布和动态分析

• 发表时间: 2006
• 期刊: JAEA-Review 2005-1
• 作者: Nakano T;Hasegawa M;Arakawa K;et al.
• 通讯作者: et al.

Analysis of radiation-induced cell death in vivo by micro particle-induced X-ray emission (micro PIXE)

通过微粒诱导的 X 射线发射 (micro PIXE) 分析辐射诱导的体内细胞死亡

• 发表时间: 2007
• 期刊: JAEA-Review 2006-42
• 作者: Hasegawa M;Sakurai H;et al.
• 通讯作者: et al.

Carbon Ion Beam-Induced Apoptosis and Growth Delay of Human Tumors in Nude Mice

碳离子束诱导裸鼠人类肿瘤细胞凋亡和生长延迟

• 发表时间: 2006
• 期刊: Int J Radiat Oncol Biol Phys 66・3S
• 作者: Hasegawa M;et al.
• 通讯作者: et al.