Elucidation of Intracellular Signal Transduction Pathway Involved in the Processing of Nociceptive Information -- Focusing on the Role of Neurotrophic Factor --

阐明参与伤害性信息处理的细胞内信号转导途径——聚焦神经营养因子的作用——

基本信息

  • 批准号:
    09671581
  • 负责人:
  • 金额:
    $ 1.66万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1997
  • 资助国家:
    日本
  • 起止时间:
    1997 至 1998
  • 项目状态:
    已结题

项目摘要

1. In the chronic constriction nerve injury model, the time course of changes in hyperalgesia and allodynia were quantitatively assessed and the high correlation between this time course and that of morphological changes in GABAergic inhibitory interneurons in the spinal dorsal horn was proved. The number and immunoreactivity of these GABAergic interneurons begin to decrease ipsiaterally at 3 days following nerve injury and no immunoreactivity could be observed bilaterally during the 3 weeks following injury. In the contralateral side, GABAergic immunoreactivity recovered approximately to the presurgical level at about 7 weeks after injury ; on the other hand in the ipsilateral side the recovery was incomplete even at 7 weeks following surgery. The time course of changes in the degenerated neurons in the spinal dorsal horn (Dark neurons) was similar to that of these changes.2. In the spinal nerve injury model, the time course of changes in GABAergic and glycinergic inhibitory interneurons in the superficial dorsal horn of the spinal cord were observed. A slight decrease in GABA immunoreactivity was observed bilaterally 3 days following surgery. Bilateral decrease in GABA immunoreactivity was accelerated at I week and continued for at least 3 weeks after surgery, when GABAergic immunoreactive components began to recover. Glycinergic fibers and terminals diminished 1 week ater nerve injury and the time course of the changes followed a similar course.3. One of the neurotrophic factors, saposin was proved to be effective in the alleviation of some pain behavior in neuropathic pain models.4. In order to evaluate the effect of various neurotrophic factors, adrenal medurally transplantation was performed using the CCI model. The therapeutic effect of adrenal medullary transplants on the vulnerable inhibitory interneurons in the spinal dorsal horn was proved and acceralated recovery of behavioral hyperalgesia and allodynia was evident.
1.在慢性缩窄神经损伤模型中,定量评估了痛觉过敏和异常性疼痛变化的时程,并证明了该时程与脊髓背角GABA能抑制性中间神经元形态变化的高度相关性。这些 GABA 能中间神经元的数量和免疫反应性在神经损伤后 3 天开始同侧减少,并且在损伤后 3 周内没有观察到双侧免疫反应性。在对侧,GABA能免疫反应性在损伤后约7周恢复到大约术前水平;另一方面,即使在手术后 7 周,同侧的恢复也不完全。脊髓背角退化神经元(暗神经元)的变化时间过程与这些变化相似。 2.在脊髓神经损伤模型中,观察脊髓背角浅层GABA能和甘氨酸能抑制性中间神经元的变化时间过程。术后 3 天观察到双侧 GABA 免疫反应性略有下降。双侧 GABA 免疫反应性下降在术后第 1 周加速,并持续至少 3 周,此时 GABA 能免疫反应性成分开始恢复。神经损伤后1周,甘氨酸能纤维和末梢减少,其变化的时间过程也遵循相似的过程。3.神经营养因子之一的saposin在神经病理性疼痛模型中被证明可以有效缓解某些疼痛行为。 4.为了评价各种神经营养因子的作用,采用CCI模型进行肾上腺髓质移植。肾上腺髓质移植对脊髓背角脆弱的抑制性中间神经元的治疗效果已得到证实,并且行为痛觉过敏和异常性疼痛的加速恢复是明显的。

项目成果

期刊论文数量(30)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
T.Ibuki,A.T.Hama,X.-T.Wang,G.D.Pappas and J.Sagen: "Loss of GABA-Immunoreactivity in the spinal dorsal horn of rats with peripheral nerve injury and promotion of recovery by adrenal medullary grafts" NeuroScience. 76(3). 845-858 (1997)
T.Ibuki、A.T.Hama、X.-T.Wang、G.D.Pappas 和 J.Sagen:“周围神经损伤大鼠脊髓背角 GABA 免疫反应性的丧失以及肾上腺髓质移植物促进恢复”《神经科学》。
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    0
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T.Ibuki and Y.Tanaka: "Changes of GABA ergic and glycinergic inhibitory neuronal system following nerve injury" Proc Sec Neurosci 2. 1392 (1998)
T.Ibuki 和 Y.Tanaka:“神经损伤后 GABA 能和甘氨酸能抑制神经元系统的变化” Proc Sec Neurosci 2. 1392 (1998)
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    0
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T.Ibuki, M.Marsala and T.L.Yaksn: "General ancsthesia inhibits the release of spinal amino acids and prostoglendin E_2 and precipitated withchcowal in inorphine" Submission.
T.Ibuki、M.Marsala 和 T.L.Yaksn:“全身麻醉抑制脊髓氨基酸和 Prostoglendin E_2 的释放,并沉淀异诺啡中的 chcowal”提交。
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    0
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T.Ibuki, A.T.Hama, G.D.Pappas and J.Sagen: "Spinal GABA ergic neurons involved in the process of rcuropathy and therapentic effects of adrenal medullary transplantation" Proc Soc Neurosc. 1533 (1997)
T.Ibuki、A.T.Hama、G.D.Pappas 和 J.Sagen:“脊髓 GABA 能神经元参与肾上腺髓质移植的肾病过程和治疗效果”Proc Soc Neurosc。
  • DOI:
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    0
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T.Ibuki,M.Morsala and T.L.Yaksn: "General anesthesia inhibits the release of spinal amino acids and prostaglandin E2 and precipitated withdrawal in inorphine tolerant rats." Submission.
T.Ibuki、M.Morsala 和 T.L.Yaksn:“全身麻醉会抑制脊髓氨基酸和前列腺素 E2 的释放,并导致耐受伊诺啡的大鼠加速戒断。”
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IBUKI Takae其他文献

IBUKI Takae的其他文献

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{{ truncateString('IBUKI Takae', 18)}}的其他基金

The involvement of cytokines in the signal transmission mechanism between immune system and central nervous system -Using the inflammatory hyperalgesia model-
细胞因子参与免疫系统与中枢神经系统信号传递机制-利用炎症性痛觉过敏模型-
  • 批准号:
    15591656
  • 财政年份:
    2003
  • 资助金额:
    $ 1.66万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Elucidation of signal transmission Mechanism from the immune system to the nervous system -Using the inflammatory pain model
阐明从免疫系统到神经系统的信号传递机制-使用炎性疼痛模型
  • 批准号:
    12671497
  • 财政年份:
    2000
  • 资助金额:
    $ 1.66万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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2024 Signal Transduction in Engineered Extracellular Matrices Gordon Research Conference and Seminar; Southern New Hampshire University, Manchester, New Hampshire; 20-26 July 2024
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