Study of vesicles produced from Porptyromonas gingvalis as a putative virulence factor

牙龈卟啉单胞菌产生的囊泡作为假定毒力因子的研究

• 批准号: 09671884
• 负责人: KAMINISHI Hidenori
• 金额: $ 2.05万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671884/
• 关键词: P. gingvalis; vesicle; pathogenic factor; periodontal disease; nitrogen oxide (NO); P.gingivalis; 微生物学; 成人性歯周炎

Extracellular vesicles of Porptyromonas gingivalis were isolated and studied to elucidate the pathogenesis. The vesicles were obtained from anaerobic culture, after differential centrifugation, and ultrafiltration using Amicon membrane filter XM300. When observed by electron microscopy, vesicles ware 30 to 450nm in diameter, and surrounded by outer membrane of a host cell which containing LPS. The vesicles induced production of inflammatory cytokines, IL-6 and TNF α , and type 1 collagenase in cultured NHDF-731 cells, except of IL-1β. When RAW264 cells were stimulated with the vesicle, the cells produced nitrogen oxide (NO) into a culture medium in the dose dependent manner. The results indicate that the vesicles from P. gingivalis had high potency of vilurence, and it thought to be not only a fragment of the cell wall but also an inducing trigger of inflammatory cytokines, nitrogen oxide and tissue degrading enzyme in host tissues. Thus, the vesicle may play as an important vilurence factor in the progress of periodontal diseases.

为阐明其致病机理，分离并研究了牙龈卟啉单胞菌胞外小泡。囊泡经厌氧培养、差速离心、Amcon膜过滤器XM300超滤后得到。在电子显微镜下观察，囊泡直径为30~450 nm，被含有内毒素的宿主细胞的外膜包围。囊泡可诱导NHDF731细胞产生炎性细胞因子IL-6、肿瘤坏死因子α和I型胶原酶，但不能产生IL-1β。当RAW264细胞被囊泡刺激时，细胞以剂量依赖的方式向培养液中产生一氧化氮(NO)。结果表明，牙龈假单胞菌的囊泡具有很高的致病活性，它不仅是细胞壁的一部分，而且是宿主组织中炎性细胞因子、一氧化氮和组织降解酶的诱导触发物。因此，水泡可能在牙周病的发展过程中起着重要的辅助作用。

项目成果

Tamaki,Cho.他: "Fungal Cells in Biodefense Mechanism"Suzuki,S.and Suzuki,M.. 317(41-45) (1997)

Tamaki, Cho 等人：“生物防御机制中的真菌细胞”Suzuki, S. 和 Suzuki, M.. 317(41-45) (1997)

Kamisishi,H. 他: "Recentprogress on Kinis,part II" Bonner,G.,Fritz,H.,Schoenlkens.,B.and Dietze,G., 492(362-369) (1992)

Kamisishi, H. 等人：“Kinis 的最新进展，第二部分”Bonner, G.、Fritz, H.、Schoenlkens., B. 和 Dietze, G., 492(362-369) (1992)

Tamaki, Cho. 他: "Fungal Cells in Biodefense Mechanism(41-45)"Suzuki, S, and Suzuki, M.. 317 (1997)

Tamaki, Cho 等人：“生物防御机制中的真菌细胞 (41-45)” Suzuki, S 和 Suzuki, M.. 317 (1997)

T. Hirota: "Induction of collagenase and cytokine mRNAs in human fibroblast stimulated with vesicles, prorease and LPS from Porptyromonas gingvalis."J. Fukuoka Dent. Coll.. 25. 51-65

T. Hirota：“用来自牙龈卟啉单胞菌的囊泡、蛋白酶和 LPS 刺激的人成纤维细胞中胶原酶和细胞因子 mRNA 的诱导。”

R. Yamaguchi, Y. Itoh, H. Ogasawara, H. Shinzi, H. Kaminishi and W. Motokawa: "In vitro study for application of electrolyzed oxidizing water to the clinical pediatric dentistry."Jpn. J. Pediatric Dent.. 35. 638-642 (1997)

R. Yamaguchi、Y. Itoh、H. Ogasawara、H. Shinzi、H. Kaminishi 和 W. Motokawa：“电解氧化水在临床儿科牙科中应用的体外研究。”Jpn。