Induction of inlammatory cytokines by stimulation of microbial proteinase and proteinaseinhibitor complex

通过刺激微生物蛋白酶和蛋白酶抑制剂复合物诱导炎症细胞因子

• 批准号: 07672005
• 负责人: KAMINISHI Hidenori
• 金额: $ 1.6万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672005/
• 关键词: P.gingivalis; proteinase; proteinaseinhibitor; IL-1beta; IL-6; fibroblast; P.gingivalis; IL-1β; Porphyromonas gingivalis; プロテアーゼ; 炎症; 歯周疾患; サイトカイン

Extracelullae proteinase from porphyromonas gingivalis were studied to elucidate the abilities of cytotoxicity and induction of cytokines and type I collagenase in human fibroblast cells. The fibroblasts were killed by the proteinase in a dose dependent manner. When the cells were treated with proteinase in the presence of fetal bovine serum or alpha2-macroglobulin, the damage of the cells was reduced but resulted in death under the prolonged culture.A semi-lethal amount of P.gingivalis proteinase induced expression of mRNAs specific for interleukin-1beta (IL-1beta) and IL-6 in the human fibroblasts with a RT-PCR analysis. It was suggested that the proteinase from P.gingivalis is not only a tissue degrading enzyme but also an inducing trigger of inflammatory cytokines. Thus the proteinase from P.gingivalis is regarded as an important virulence factor and may play an important role in the progress of periodontal diseases.

研究牙龈卟啉单胞菌胞外蛋白水解酶对人成纤维细胞的细胞毒作用及对细胞因子和I型胶原酶的诱导作用。成纤维细胞被该酶以剂量依赖的方式杀死。在含有胎牛血清或α2-巨球蛋白的情况下，用蛋白酶处理细胞，可减轻细胞损伤，但延长培养时间可导致细胞死亡。半致死量的牙龈假单胞菌蛋白水解酶可诱导人成纤维细胞表达IL-1β和IL-6特异性的mRNAs。提示牙龈假单胞菌的蛋白水解酶不仅是一种组织降解酶，而且是一种诱导炎性细胞因子的酶。因此，牙龈假单胞菌的蛋白水解酶被认为是一种重要的毒力因子，可能在牙周病的发生发展中起重要作用。

项目成果

廣田 健 他: "歯垢からのsiderophore産生菌検出" 福岡歯科大学学会雑誌. 22・3. 439-444 (1995)

Ken Hirota等人：“牙菌斑中产生副载体的细菌的检测”福冈齿科大学学报22・3（1995）。

Kaminishi,H. 他: "Degradation of Humoral Host Defense by Candida albicans proteinase" Infection and Immunity. 63・3. 984-988 (1995)

Kaminishi, H. 等人：“白色念珠菌蛋白酶对体液宿主防御的降解”感染和免疫 63・3 (1995)。

久松雅彦他: "実験的マウスカンジダ症におけるペプスタチンの効果" 医学と生物学. 132・4. 191-194 (1996)

Masahiko Hisamatsu 等：“胃酶抑素对实验性鼠念珠菌病的影响”医学和生物学 191-194（1996）。

Kaminishi,H.他: "Spinal hyphae of Candida albicans formed in anaerobic culture" Mycoses. 37. 349-352 (1994)

Kaminishi, H. 等人：“厌氧培养中形成的白色念珠菌的脊髓菌丝”Mycoses 37. 349-352 (1994)。

Hisamatu, M., Matumoto, H., Mukai, M.et al.: "Effect of pepstatin on an experimental murine candidosis" Medicine and Biology. 132 (4). 191-194 (1996)

Hisamatu, M.、Matumoto, H.、Mukai, M.等人：“胃酶抑素对实验性小鼠念珠菌病的影响”医学和生物学。