Regulation Mechanism of Gene Expression by Insect Juvenile Hormone and Identification of Juvenile Hormone Receptor

昆虫保幼激素基因表达调控机制及保幼激素受体鉴定

• 批准号: 10044203
• 负责人: CHINZEI Yasuo
• 金额: $ 4.61万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044203/
• 关键词: Juvenile Hormone; ecdysone; metamorphosis; transcription factor; GRF; ECR; USP; JH receptor; リセプター; 遺伝子発現; 転写調節因子; シアノプロテイン; ヴィテロジェニン

Insect juvenile hormone (JH) is an important hormone for insect to regulate metamorphosis, diapause, polymorphism, reproduction and so on. Molecular mechanism of mode of action of JH have been studied, and shown to regulate the expression of genes related to those physiological phenomena. However, JH receptor has not been identified and isolated.A PCR approach has been used to isolate, form Bombyx mori, a cDNA encoding a novel orphan receptor (GRF) that is most closely related to Bombyx bFTZ-F1 and to the vertebrate germ cell nuclear factor. The major GRF mRNA is detected in most tissues as an 8-kb transcript whose amount follows the circulating ecdysteroid concentration with a delay. The expression pattern of GRF is similar to that of the Bombyx homologue of the Drosophila early late gene DHR3, and precedes that of bFTZ-F1 in all stages and tissues examined. The GRF protein is thus likely to be required in many tissues, but in a temporally restricted manner suggesting that GRF has a well-defined function in the ecdysteroid-induced transcription cascade. The GRF protein binds in vitro to a single oestrogen receptor half-site AGGTCA preceded by a 50 -TCA extension, and is therefore a potential co-regulator of the orphan receptors bFTZ-F1 and DHR39.

昆虫保幼激素（Insect Juvenile Hormone，JH）是调节昆虫变态、滞育、多态性、生殖等生理现象的重要激素，其作用机制的分子生物学研究表明，JH可调节与这些生理现象相关的基因的表达。然而，JH受体尚未被鉴定和分离。PCR方法已被用于从家蚕中分离编码与家蚕bFTZ-F1和脊椎动物生殖细胞核因子最密切相关的新型孤儿受体（GRF）的cDNA。在大多数组织中检测到主要的GRF mRNA作为8-kb转录物，其量随循环蜕皮激素浓度延迟而变化。GRF的表达模式类似于果蝇早晚期基因DHR 3的pGEX同源物的表达模式，并且在所检查的所有阶段和组织中先于bFTZ-F1。因此，GRF蛋白可能是需要在许多组织中，但在时间上受到限制的方式表明，GRF具有明确的功能，在蜕皮激素诱导的转录级联。GRF蛋白在体外结合单个雌激素受体半位点AGGTCA，之前是50 -TCA延伸，因此是孤儿受体bFTZ-F1和DHR 39的潜在共调节因子。

项目成果

Ken Miura, Tetsuro Shinoda, Mamoru Yura, Sen Nomura, Katsumi Kamiya, Masao Yuda and Yasuo Chinzei: "Two hexameric cyanoprotein subunits from an insect, Riptortus clavatus : Sequence, Phylogeny"Eur. J. Biochem.. 258. 929-940 (1998)

Ken Miura、Tetsuro Shinoda、Mamoru Yura、Sen Nomura、Katsumi Kamiya、Masao Yuda 和 Yasuo Chinzei：“来自昆虫 Riptortus clavatus 的两个六聚氰蛋白亚基：序列、系统发育”Eur。

Jean-Philippe Charles et al.: "Characterization and DNA-binding properties of GRF, a novel monomeric binding orphan receptor related to GCNF and βFTZ-F1"Eur. J. Biochem.. 266. 181-190 (1999)

Jean-Philippe Charles 等人：“GRF 的特征和 DNA 结合特性，一种与 GCNF 和 βFTZ-F1 相关的新型单体结合孤儿受体”Eur. J. Biochem. 266. 181-190 (1999)

Jean-Philippe Charles, Tetsuro Shinoda and Yasuo Chinzei: "Characterization and DNA-binding properties of GRF, a novel monomeric binding orphan receptor related to GCNF and βFTZ-F1"Eur. J. Biochem.. 266. 181-190 (2000)

Jean-Philippe Charles、Tetsuro Shinoda 和 Yasuo Chinzei：“GRF 的特征和 DNA 结合特性，一种与 GCNF 和 βFTZ-F1 相关的新型单体结合孤儿受体”Eur. J. Biochem.. 266. 181-190 (2000)

Makoto Hirai et al.: "Identification and cDNA cloning of novel juvenile hormone responsive genes from fat body of bug,Riptortus clavatus by mRNA differential display"Insect Biochem.Mol.Biol.. 28. 181-189 (1999)

Makoto Hirai 等：“通过 mRNA 差异显示从昆虫脂肪体中鉴定和 cDNA 克隆新型保幼激素反应基因，Riptortus clavatus”Insect Biochem.Mol.Biol.. 28. 181-189 (1999)

J.Sun et al.: "Characterization and cDNA cloning of a hemoprotein in the salivary glands of the blood-sucking insect,Rhodnius prolixus." Insect Biochem.Mol.Biol.28. 191-200 (1998)

J.Sun 等人：“吸血昆虫 Rhodnius prolixus 唾液腺中血红素蛋白的表征和 cDNA 克隆。”