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Sage specific host cell infection mechanism of malarial parasite

疟疾寄生虫的鼠尾草特异性宿主细胞感染机制

基本信息

批准号：
14207011
负责人：
CHINZEI Yasuo
金额：
$ 28.12万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14207011/
关键词：
malarial parasite ookinete sporozoite infection mechanism rodent malaria Anpheline mosquito Kupffer cell liver infection マラリア EST解析ノックアウト原虫肝臓感染機構接着侵入分子 ESTデータベース

项目摘要

The summary of this project performed in this three years is shown below. Malarial parasite is transmitted by Anopheline mosquito. Parasite ookinete and sporozoite invade and pass through the mosquito mig-gut epithlium cell and the salivary gland cell, and formed in the mosquito mid gut lumen by sexual fusion passes through the mid-gut epithelium and forms a oocyst on the gut wall Salivary gland sporozoite injected into the host by mosquito bite has to pass through the skin barriers and lliver sinusoidal cell layer to reach and infect the hepatocyte. We planned to investigate the molecular mechanisms of the parasite invasion into those barrier cells. For this purpose, we used a rodent malarial parasite Plasmodium berghei as a model animal, and analyzed the EST (expressed sequence tags) of ookinete and sporozoite. We selected gene by a criteria from the EST and generated parasite disrupted the gene. A function of the gene was studied by the analyses of the phenotype of the gene disrupted parasite. As results, we identified MAOP and MAEBL for essential molecules for invasion to mig-gut epithelial cell and the salivary gland cell. And we also identified two molecules, SPECT1 and 2 for essential molecules to invade int the Kupffer cell in the sinusoidal cell layer of the liver. We identified OSM-1, a molecule essential for translocation through the mid-gut cell and Kupffer cell from the ookinete and sporozoite. We demonstrated the common mechanisms of the cell passage of ookinete and sporozoite through the barrier cell layer, the mosquito mid-gut cell and the sinusoidal cell layer. We also demonstrated first for the sporozoite to pass through the Kupffer cell in the sinusoidal layer to reach the hepatocyte to infect from the sinusoid.

这三年来执行的项目摘要如下。疟疾是由按蚊传播的。寄生虫动卵细胞和子孢子侵入并穿过蚊的移行肠上皮细胞和唾液腺细胞，在蚊的中肠腔中通过性融合形成，穿过中肠上皮细胞在肠壁上形成卵囊。我们计划研究寄生虫侵入这些屏障细胞的分子机制。为此，我们使用啮齿类疟原虫伯氏疟原虫作为模式动物，并分析了动合子和子孢子的EST（表达序列标签）。我们从EST中筛选基因，并产生了破坏该基因的寄生虫。通过对基因破坏的寄生虫的表型分析，研究了该基因的功能。结果表明，MAOP和MAEBL是侵袭小肠上皮细胞和唾液腺细胞的必需分子。我们还鉴定了两种分子，SPECT 1和2，它们是入侵肝脏窦状细胞层枯否细胞的必需分子。我们确定了OSM-1，一种从动合子和子孢子通过中肠细胞和库普弗细胞易位所必需的分子。我们证明了动合子和子孢子通过屏障细胞层、蚊子中肠细胞和窦状细胞层的共同机制。我们还首次证明了子孢子可以通过肝窦层的枯否细胞到达肝细胞，从肝窦感染。

项目成果

期刊论文数量（19）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

W.Limviroj, K.Yano, M.Yuda, K.Ando, Y.Chinzei: "Immuno-electron microscopic observation of Plasmodium berghei CTRP localization in the midgut of vector mosquito, Anopheles stephensi"J. Parasitol.. 88. 664-672 (2002)

W.Limviroj、K.Yano、M.Yuda、K.Ando、Y.Chinzei：“免疫电子显微镜观察伯氏疟原虫 CTRP 在媒介蚊子斯蒂芬斯按蚊中肠中的定位”J。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Essential role of membrane-attack protein in malarial transmission to mosquito host

DOI：
10.1073/pnas.0406187101
发表时间：
2004-11-16
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Kadota, K;Ishino, T;Yuda, M
通讯作者：
Yuda, M

S.Iwanaga, M.Okada, H.Isawa, A.Morita, M.Yuda, Y.Chinzei: "Identification and characterization of novel salivary thrombin inhibitors from the ixodidae tick, Haemaphysalis longicornis"Eur. J. Biochem.. (in press). (2003)

S.Iwanaga、M.Okada、H.Isawa、A.Morita、M.Yuda、Y.Chinzei：“来自 ixodidae 蜱、Haemaphysalis longicornis 的新型唾液凝血酶抑制剂的鉴定和表征”Eur。