Infecton Mechanisms of Malarail Parasite Sporozoites to the Liver Cells
Malarail寄生虫子孢子对肝细胞的感染机制
基本信息
- 批准号:16017243
- 负责人:
- 金额:$ 9.6万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research on Priority Areas
- 财政年份:2004
- 资助国家:日本
- 起止时间:2004 至 2005
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Malarial parasites have very complicated life cycle. There are many steps for the parasites to invade into the host tissues or cells of both vector mosquito and vertebrate animal. We are interested in molecular mechanisms of the parasite invasion of host tissues. We have analyzed parasite proteins essential for invasion into host mosquito midgut and host liver using the rodent malaria Plasmodium berghei. We established EST (expressed sequence tags) databases of ookinetes and sporozoites, selected some molecules based on certain criteria, and analyzed their function by gene targeting disruption. We have elucidated several molecules that function in parasite infection (movement, recognition, invasion, development and/or proliferation) of host. We identifiedand and named as CTRP (CS TRAP Related Protein) and MAOP (Membrane Attack Ookinete Protein) from sporozoite and ookinete stages of parasites. Both proteins have membrane attack complex /perforin domain in the molecule and function to rupture the cell membrane of Kuppfer cell in the liver sinusoidal layer and mosquito midgut cell respectively. Ookinetes and sporozoites have cellular barriers, midgut epithelial cells of mosquitoes for ookinetes and the sinusoidal layer cells in the liver for sporozoites to reach their respective infective sites. Malarial parasites use different homologous molecules, and a common conserved cell traversal
疟疾寄生虫的生活史非常复杂。寄生虫入侵媒介蚊子和脊椎动物的宿主组织或细胞需要许多步骤。我们对寄生虫入侵宿主组织的分子机制感兴趣。我们使用啮齿动物伯氏疟原虫分析了入侵宿主蚊子中肠和宿主肝脏所必需的寄生虫蛋白。我们建立了动子和子孢子的表达序列标签(EST)数据库,根据一定的标准筛选出一些分子,并通过基因打靶干扰来分析它们的功能。我们已经阐明了几个在寄生虫感染(宿主的移动、识别、入侵、发育和/或增殖)中起作用的分子。我们从寄生虫的子孢子和动粒体期中鉴定并命名为CTRP(CS TRAP Related Protein)和MAOP(膜攻击动子蛋白)。这两种蛋白在分子中都有膜攻击复合体/穿孔素结构域,并分别在肝窦层和蚊子中肠细胞中起到破坏Kuppfer细胞膜的作用。动子和子孢子有细胞屏障,蚊子的中肠上皮细胞对动子有屏障,肝脏的肝窦层细胞对子孢子到达各自的感染部位有保护作用。疟疾寄生虫使用不同的同源分子,以及共同的保守细胞遍历
项目成果
期刊论文数量(26)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Plasmodium sporozoite micronemal protein with a membrane attack complex-related domain is required for breaching the liver sinusoidal cell layer prior to hepatocyte infection.
具有膜攻击复合物相关结构域的疟原虫子孢子微线蛋白是在肝细胞感染之前突破肝窦细胞层所必需的。
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Tomoko Ishino;Yasuo Chinzei;Masao YudaA
- 通讯作者:Masao YudaA
Two proteins with 6-cys motifs are required for malarial parasites to commit to infection of the hepatocyte
- DOI:10.1111/j.1365-2958.2005.04801.x
- 发表时间:2005-12-01
- 期刊:
- 影响因子:3.6
- 作者:Ishino, T;Chinzei, Y;Yuda, M
- 通讯作者:Yuda, M
(* : equally contributed) CelTOS, a novel malarial protein that mediates transmission to mosquito and vertebrate hosts.
(*:同等贡献)CelTOS,一种新型疟疾蛋白,可介导向蚊子和脊椎动物宿主的传播。
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Tohru Kariu*;Tomoko Ishino*;Kazuhisa Yano;Yasuo Chinzei;Masao Yuda.
- 通讯作者:Masao Yuda.
Two proteins with 6-cys motifs are required for malarial parasites to commit to infection of the hepatocyte.Mol.
疟疾寄生虫需要两种具有 6-cys 基序的蛋白质来感染肝细胞。
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Tomoko Ishino;Yasuo Chinzei;Masao Yuda
- 通讯作者:Masao Yuda
CelTOS, a novel malarial protein that mediates transmission to mosquito and vertebrate hosts
- DOI:10.1111/j.1365-2958.2005.05024.x
- 发表时间:2006-03-01
- 期刊:
- 影响因子:3.6
- 作者:Kariu, T;Ishino, T;Yuda, M
- 通讯作者:Yuda, M
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CHINZEI Yasuo其他文献
CHINZEI Yasuo的其他文献
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{{ truncateString('CHINZEI Yasuo', 18)}}的其他基金
Malarial parasite molecules essential for development from sporoziote to merozoite in the liver stage.
疟疾寄生虫分子对于肝脏阶段从子孢子发育到裂殖子至关重要。
- 批准号:
21590472 - 财政年份:2009
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Studies on the Mechanisms of Stage specific Host Infection in Malarial Parasite
疟原虫阶段特异性宿主感染机制研究
- 批准号:
17209014 - 财政年份:2005
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Sage specific host cell infection mechanism of malarial parasite
疟疾寄生虫的鼠尾草特异性宿主细胞感染机制
- 批准号:
14207011 - 财政年份:2002
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Function and Structure Analyses of Anticoagulant and Vasodilator Isolated from the Salivary Glands of Blood Sucking Insects, and Development of Novel Medicine
吸血昆虫唾液腺抗凝剂和血管扩张剂的功能和结构分析及新药开发
- 批准号:
11557022 - 财政年份:1999
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Regulation Mechanism of Gene Expression by Insect Juvenile Hormone and Identification of Juvenile Hormone Receptor
昆虫保幼激素基因表达调控机制及保幼激素受体鉴定
- 批准号:
10044203 - 财政年份:1998
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Identification and cloning of JH receptor
JH受体的鉴定与克隆
- 批准号:
10460020 - 财政年份:1998
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Juvenile hormone receptor and molecular mechanism of gene expresion
保幼激素受体及基因表达的分子机制
- 批准号:
08456171 - 财政年份:1996
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanism of gene expresion regulated by juvenile hormone : Identification and its function of Juvenile hormone receptor, responsive elements and transcription factor
保幼激素调控基因表达的分子机制:保幼激素受体、反应元件和转录因子的鉴定及其功能
- 批准号:
07044192 - 财政年份:1995
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for international Scientific Research
Anticoagulant of blood sucking insect salivary glands
吸血昆虫唾液腺抗凝剂
- 批准号:
06556010 - 财政年份:1994
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Molecular Mechanism of Juvenile Hormone Action
保幼激素作用的分子机制
- 批准号:
04454060 - 财政年份:1992
- 资助金额:
$ 9.6万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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疟疾寄生虫的鼠尾草特异性宿主细胞感染机制
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14207011 - 财政年份:2002
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