High resolution confocal microscope

行为神经科学工作组代表生物与生物技术学院申请一台高分辨率共聚焦显微镜，以分析神经退行性疾病的细胞机制和神经回路。行为神经科学工作组和普通动物学和神经生物学系长期以来一直有兴趣确定不同的G蛋白偶联的第二信使球员和离子通道，负责使人衰弱的疾病，如共济失调，癫痫，创伤后应激障碍和失明的活组织和行为小鼠。我们创建并利用现有的人类疾病，发作性共济失调2型，脊髓小脑共济失调6型和视网膜变性的小鼠模型，以了解这些疾病状态的潜在机制。通过应用最先进的光遗传学，化学遗传学，遗传编码荧光传感器和钙成像，电生理学和行为技术的组合，我们有强大的能力来解决和解剖这些复杂的疾病状态，这将大大受益于高分辨率的显微系统。高分辨率设备将能够从表达多达6种不同荧光蛋白（光谱分离/解混）的亚细胞器和聚集体（高分辨率）进行长期活细胞成像，同时去除自体荧光（自体荧光校正功能）。目前有一个高的需求和需要高分辨率的共聚焦显微镜在生物和生物技术学院，我们目前的共聚焦显微镜不能满足。所要求的高分辨率显微镜将是一个多用户设施，可供生物学、生物技术和信息学系以及鲁尔大学波鸿的所有教职员工使用。

On behalf of the Faculty of Biology and Biotechnology, the Working Group of Behavior Neuroscience would like to apply for a high-resolution confocal microscope to analyze the underlying cellular mechanisms and neural circuitry underlying neurodegenerative diseases. The Working Group of Behavior Neuroscience and Department of General Zoology and Neurobiology have a long-standing interest in identifying the different G protein coupled second messenger players and ion channels responsible for debilitating diseases such as ataxia, epilepsy, post-traumatic stress disorder and blindness in living tissues and behaving mice. We created and utilize available mouse models to the human diseases, episodic ataxia type 2, spinocerebellar ataxia type 6 and retinal degeneration to understand the underlying mechanisms of these diseased states. By applying a combination of state-of-the-art optogenetic, chemogenetic, genetically encoded fluorescent sensor and calcium imaging, electrophysiology and behavioral techniques, we are strongly equipped to tackle and dissect out these complex disease states which would highly benefit from a high-resolution microscopic system. A high-resolution device would enable long term live cell imaging from subcellular organelles and aggregates (high-resolution) expressing up to 6 different fluorescent proteins (spectral separation/unmixing) and simultaneously removing autofluorescence (autofluorescence correction feature). Currently there is a high demand and need for a high-resolution confocal microscope in the Faculty of Biology and Biotechnology which our current confocal microscope cannot fulfill. The high-resolution microscope requested will be a multi-user facility and be available for all faculty members in the Departments of Biology and Biotechnology and Informatics, and at the Ruhr-University Bochum.