Abberior Infinity Line Upright 3D STED/Confocal Microscope

Abberior Infinity Line 正置 3D STED/共焦显微镜

• 批准号: 10632948
• 负责人: Anthony J Ricci
• 金额: $ 145.47万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-15 至 2024-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10632948
• 关键词: 3-Dimensional; Aging; Basic Science; Biological; Clinical; Collaborations; Core Facility; Data Analyses; Detection; Development; Devices; Diagnostic; Equipment; Experimental Designs; Fostering; Health; Image; Lasers; Light; Lighting; Maintenance; Malignant Neoplasms; Microscope; Mission; Noise; Optics; Otolaryngology; Pharmaceutical Preparations; Positioning Attribute; Postdoctoral Fellow; Research; Research Personnel; Resolution; Resources; Rotation; Running; Schedule; Scientist; Services; Signal Transduction; System; Technical Expertise; Technology; Training; United States National Institutes of Health; Work; adaptive optics; detector; experience; fluorophore; imaging system; improved; live cell imaging; nervous system disorder; next generation; sensory integration; student participation; superresolution imaging; technology development; ultra high resolution

Abstract: The Department of Otolaryngology has active research across all of its divisions. The department has run a core facility for over 12 years that is accessible to more than 100 researchers within our department. The core facility is a training facility that fosters collaboration across technical expertise and biological questions. We are requesting the purchase of a super- resolution imaging system that will be placed into this core facility where training, scheduling and maintenance can be managed by experienced staff. The Abberior STED system incorporates the most up-to-date technology to provide the most versatile super- resolution system available. The use of adaptive optics and adaptive illumination allows for high-resolution live cell imaging. The incorporation of avalanche diodes provides the most sensitive detectors available. Quad scanners maintain beam positioning and alignment while providing image rotation. Matrix array spectral detection offers the next generation of improved signal to noise by removing out focus light. STED FLIM allows for lower power laser to improve live-cell imaging. It further provides optical colocalization by using a single light path for multiple emissions. Multiple emissions are possible by separating fluorophores by lifetime rather than wavelength. There are no existing systems at Stanford so this new device will be critical for many scientists both within and outside of our department. Work within our department spans from basic science to translational to clinical. It ranges from cancer to aging, to neurological disorders, sensory integration and drug and diagnostic development. It is directly in line with the mission of NIH in terms of a primary focus on health-related research and training of the next-generation of scientists. Our core facility also serves as a training facility where we engage students, postdocs, residents, etc. and train them on the first principles of the technology development, experimental design and data analysis. Finally, the Department of Otolaryngology supports the core facility and will support this new addition by providing the space and the resources needed for staff as well as maintenance, service and oversight of the equipment.

摘要： 耳鼻喉科的部门在其所有部门都有积极的研究。的 部门已经运行了超过12年的核心设施，可供100多人使用 我们部门的研究人员。核心设施是一个训练设施， 在技术专长和生物学问题方面开展合作。我们请求 购买一个超分辨率成像系统，将被放置在这个核心设施 培训、调度和维护可由经验丰富的员工管理。的 Abberior STED系统采用了最先进的技术， 多功能超分辨率系统可用。自适应光学和自适应光学的应用 照明允许高分辨率的活细胞成像。雪崩的合并 二极管提供了可用的最灵敏的检测器。四扫描仪保持光束 定位和对准，同时提供图像旋转。矩阵阵列光谱检测 通过去除散焦光提供下一代改进的信噪比。STED FLIM允许较低功率的激光来改善活细胞成像。它还提供光学 通过使用用于多个发射的单个光路进行共定位。多重排放是 通过按寿命而不是波长分离荧光团是可能的。没有 斯坦福大学现有的系统，所以这个新设备将是至关重要的许多科学家， 在我们部门内外。我们部门的工作范围从基本的 从科学到临床从癌症到衰老， 疾病、感觉统合以及药物和诊断的发展。它直接符合 与NIH的使命一致，主要关注与健康相关的研究和培训 下一代科学家的梦想我们的核心设施也作为一个培训设施， 我们吸引学生，博士后，居民等，并对他们进行培训的首要原则， 技术开发、实验设计和数据分析。最后，该部 耳鼻喉科支持核心设施，并将通过提供 工作人员所需的空间和资源以及维护、服务和 设备的监督。