Study on single molecule mechanobiology of microtubule

点击翻译按钮获取中文摘要

The current research hypothesizes that microtubules are mechanotransducers of mechanical stress. In this study, a comprehensive understanding of the issue will be made. Last year the modulation of cargo transport in response to mechanical stress on the microtubule is a universal phenomenon independent of the motor protein by using kinesin was verified. Kinesin-driven transport of fluorescent quantum dots was carried out on microtubules fixed on a stretchable substrate. The effect of the mechanical stress on the cargo transport was systematically evaluated by applying compressive strain or tensile strain using the microstretcher chamber developed previously. Parameter analysis e.g., run length, velocities, pause frequencies, etc., from the fluorescence microscopy observation was done.