Study on single molecule mechanobiology of microtubule

微管单分子力学生物学研究

• 批准号: 22KF0208
• 负责人: 角五 彰
• 金额: $ 1.47万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2023
• 资助国家: 日本
• 起止时间: 2023-03-08 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KF0208/
• 关键词: Kinesin; Dynein; Microtubule; Mechanical stress; Mechano-transducer

The current research hypothesizes that microtubules are mechanotransducers of mechanical stress. In this study, a comprehensive understanding of the issue will be made. Last year the modulation of cargo transport in response to mechanical stress on the microtubule is a universal phenomenon independent of the motor protein by using kinesin was verified. Kinesin-driven transport of fluorescent quantum dots was carried out on microtubules fixed on a stretchable substrate. The effect of the mechanical stress on the cargo transport was systematically evaluated by applying compressive strain or tensile strain using the microstretcher chamber developed previously. Parameter analysis e.g., run length, velocities, pause frequencies, etc., from the fluorescence microscopy observation was done.

目前的研究假设微管是机械应力的机械传感器。在本研究中，将对这一问题进行全面的了解。去年，通过使用驱动蛋白证实了微管上的机械应力对货物运输的调节是一种不依赖于马达蛋白的普遍现象。驱动蛋白驱动的荧光量子点的运输在固定在可拉伸基底上的微管上进行。通过使用先前开发的微拉伸器室施加压缩应变或拉伸应变，系统地评估了机械应力对货物运输的影响。参数分析，例如，运行长度、速度、暂停频率等，从荧光显微镜观察。

项目成果

Methods in Molecular Biology, Microtubules-Methods and Protocols

分子生物学、微管方法和实验方案

• 发表时间: 2022
• 作者: Syeda Rubaiya Nasrin;Arif Md. Rashedul Kabir;Akira Kakugo
• 通讯作者: Akira Kakugo