記憶プロセスにおける海馬CA2野の動態の解明

阐明记忆过程中海马 CA2 区的动态

• 批准号: 22KF0402
• 负责人: McHugh Thomas
• 金额: $ 1.47万
• 依托单位: Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2023
• 资助国家: 日本
• 起止时间: 2023-03-08 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KF0402/
• 关键词: hippocampus; medial septum; spatial memory; locomotion; connectome

The last fiscal year focused on addressing the anatomical connectome of CA2 using viral tracing methods. To map the brain-wide connections, several transgenic mouse lines were applied, along with an unsupervised machine learning approach to reveal divergent phenotypes, especially highlighting the hippocampal-medial septum circuits. These results help to lay the foundation for designing behavioral experiments. Meanwhile, to dissect the physiological features of CA2 during memory information routing, analysis at the single-cell and population level revealed a novel role for CA2 in mediating hippocampal dynamics under discrete behavioral states. Such results were also compared and reviewed with previous works to suggest a unique role of CA2 during memory processing.

上一财年的重点是使用病毒追踪方法解决CA 2的解剖连接组。为了绘制全脑连接，应用了几种转基因小鼠品系，沿着无监督机器学习方法来揭示不同的表型，特别是突出了大脑中膜-内侧隔回路。这些结果有助于为设计行为实验奠定基础。同时，解剖在记忆信息路由过程中的CA 2的生理特征，在单细胞和群体水平的分析揭示了一个新的角色，CA 2在介导离散行为状态下的海马动力学。这些结果也进行了比较，并与以前的工作进行审查，提出了一个独特的作用，CA 2在记忆加工。