記憶回路を律動的に制御する機構の同定とモデル化

有节奏地控制记忆电路的机制的识别和建模

• 批准号: 18F18077
• 负责人: McHugh Thomas
• 金额: $ 1.47万
• 依托单位: Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2018
• 资助国家: 日本
• 起止时间: 2018-10-12 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18F18077/
• 关键词: hippocampus; theta; OLM

In the last eight months Dr. Sekulic combined behavioural, immunohistochemical and genetic approaches to induce new memories in mice and subsequently label active cells, specifically interneurons involved in the theta rhythm, representative of the novel neuronal ensemble. He evaluated the overlap of the newly marked neurons with the population of inhibitory interneurons of interest, specifically O-LM cells. The mice used were a mix of wild-type, Somatostatin-Cre (SST-Cre) mice for labeling O-LM cells and cfos-tTA and cfos-tTA/SST-Cre double positive mice for marking active neurons during new memories that might be O-LM cells. Additionally, the RAM (Robust Activity Marking) virus, a recently developed tool for marking newly active neurons, was also used in combination with SST-Cre mice. These efforts are an entirely new line of inquiry in my laboratory and I am very satisfied with his efforts and productivity. In parallel Dr. Sekulic has started his training in in vivo physiological recording to monitor the dynamic activity of this neuronal population in freely mice. He has made excellent progress and will soon begin to collect the necessary data.

在过去的八个月里，Sekulic博士结合了行为，免疫组织化学和遗传方法来诱导小鼠的新记忆，随后标记活性细胞，特别是参与theta节律的中间神经元，代表了新的神经元集合。他评估了新标记的神经元与感兴趣的抑制性中间神经元群体的重叠，特别是O-LM细胞。使用的小鼠是用于标记O-LM细胞的野生型生长抑素-Cre（SST-Cre）小鼠和用于标记可能是O-LM细胞的新记忆期间的活性神经元的cfos-tTA和cfos-tTA/SST-Cre双阳性小鼠的混合物。此外，RAM（Robust Activity Marking）病毒，一种最近开发的用于标记新活跃神经元的工具，也与SST-Cre小鼠组合使用。这些努力在我的实验室里是一个全新的探索方向，我对他的努力和生产力非常满意。与此同时，Sekulic博士开始了体内生理记录的培训，以监测自由小鼠中神经元群体的动态活动。他已经取得了极好的进展，不久将开始收集必要的数据。