記憶回路を律動的に制御する機構の同定とモデル化

在过去的八个月里，Sekulic博士结合了行为、免疫组织化学和遗传方法来诱导小鼠的新记忆，并随后标记了活跃的细胞，特别是参与theta节奏的中间神经元，代表了新的神经元组合。他评估了新标记的神经元与感兴趣的抑制性中间神经元群体的重叠，特别是O-LM细胞。使用的小鼠是野生型生长抑素-Cre(SST-Cre)小鼠的混合体，用于标记O-LM细胞和CFOS-TTA和CFOS-TTA/SST-Cre双阳性小鼠，用于标记可能是O-LM细胞的新记忆中的活跃神经元。此外，最近开发的标记新活动神经元的工具RAM(健壮活动标记)病毒也被用于SST-CRE小鼠。这些努力在我的实验室里是一种全新的调查方式，我对他的努力和生产率非常满意。与此同时，塞库里奇博士已经开始了体内生理记录方面的培训，以监测自由小鼠中这一神经元群体的动态活动。他已经取得了很好的进展，很快就会开始收集必要的数据。

In the last eight months Dr. Sekulic combined behavioural, immunohistochemical and genetic approaches to induce new memories in mice and subsequently label active cells, specifically interneurons involved in the theta rhythm, representative of the novel neuronal ensemble. He evaluated the overlap of the newly marked neurons with the population of inhibitory interneurons of interest, specifically O-LM cells. The mice used were a mix of wild-type, Somatostatin-Cre (SST-Cre) mice for labeling O-LM cells and cfos-tTA and cfos-tTA/SST-Cre double positive mice for marking active neurons during new memories that might be O-LM cells. Additionally, the RAM (Robust Activity Marking) virus, a recently developed tool for marking newly active neurons, was also used in combination with SST-Cre mice. These efforts are an entirely new line of inquiry in my laboratory and I am very satisfied with his efforts and productivity. In parallel Dr. Sekulic has started his training in in vivo physiological recording to monitor the dynamic activity of this neuronal population in freely mice. He has made excellent progress and will soon begin to collect the necessary data.