Animal model of deafness produced by partial destruction of the inner ear.

内耳部分破坏产生的耳聋动物模型。

• 批准号: 59440072
• 负责人: NOMURA Yasuya
• 金额: $ 10.56万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1985
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-59440072/
• 关键词: animal model; sensorineural hearing loss; partial destruction; inner ear

The purpose of this study was to make an animal model of sensorineural hearing loss by partial destruction of the inner ear. Argon laser was used for this particular purpose.The tympanic bulla of the guinea pig was exposed in order to irradiate laser beam into the cochlea. The cochlear wall and the round window membrane were the sites of irradiation. Power and duration of single irradiation were 1 w and 0.5 sec. Cochlear changes after irradiation to the cochlear bony wall were not uniform, because of thickness of the bony wall. However, irradiation through the round window membrane produced marked morphological changes in the cochlea: Vacuole formation in the stria vascularis, rupture or Reissner's membrane, fracture of the osseous spiral lamina, destruction of organ of Corti and destruction of the tectorial membrane. The method can be used not only to investigate correlation of morphology and function of the inner ear, but to develop a method of treatment of inner ear disease.

本研究的目的是通过部分破坏内耳的方法建立感音神经性聋的动物模型。采用氩激光照射豚鼠鼓泡，使激光束照射耳蜗。照射部位为耳蜗壁和圆窗膜。单次照射功率为1w，照射时间为0.5s。由于耳蜗骨壁的厚度，照射后耳蜗的变化不均匀。然而，通过圆窗膜照射产生了显着的形态学变化，在耳蜗血管纹中的小泡形成，破裂或Reissner膜，骨螺旋板断裂，破坏Corti器官和破坏的盖膜。该方法不仅可用于研究内耳形态与功能的相关性，而且可用于内耳疾病的治疗。