Studies on the mechanism of cadmium-induced toxic effects(Autoradiographic demonstration of cadmium unbound to metallothionein)

镉毒性作用机制研究（未与金属硫蛋白结合的镉的放射自显影证明）

• 批准号: 60570020
• 负责人: SUZUKI Takuro
• 金额: $ 1.02万
• 依托单位: St. Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570020/
• 关键词: autoradiography; cadmium; thiazolylazo-naphthol

A new autoradiographic technique for demonstrating trace amounts of "free cadmium" which remains unbound to metallothionein in a tissue section is described. This was realized by the use of [14C-1,4,5,8-naphthol]-1-(thiazolylazo)-2-naphthol (TAN) instead of a metal-radioisotope. In this method, tissue sections were stained with an aqueous solution of labeled TAN, and then the sections were placed in 2-propanol in order to remove any unreacted TAN. The decontaminated sections were finally subjected to the usual autoradiographic procedure.A high concentration of grains overlying coloured regions was observed in sections prepared from tissues injected with a diluted aqueous solution of cadmium acetate. This demonstrated the feasibility of the differential demonstration of cadmium according to its chemical forms in tissues. This technique may yield additional information as to the toxicity of cadmium in biological systems.

本文介绍了一种新的放射自显影技术，用于显示组织切片中未结合金属硫蛋白的痕量“游离镉”。这是通过使用[14 C-1，4，5，8-萘酚]-1-（噻唑偶氮）-2-萘酚（TAN）代替金属放射性同位素实现的。在该方法中，将组织切片用标记的TAN的水溶液染色，然后将切片置于2-丙醇中以除去任何未反应的TAN。最后对净化后的切片进行常规的放射自显影，在用醋酸镉稀释水溶液注射的组织制备的切片中观察到高浓度的颗粒覆盖在着色区域上。这表明了镉在组织中的化学形态差异显示的可行性。这项技术可能会产生额外的信息，镉在生物系统中的毒性。

项目成果

Y.Sumi;T.Suzuki;K.T.Suzuki: Journal of Histochemistry and Cytochemistry.

Y.Sumi；T.Suzuki；K.T.Suzuki：组织化学和细胞化学杂志。

Y. Sumi, T. Suzuki and K.T. Suzuki: "Autoradiographic demonstration of cadmium unbound to metallothionein with 14C-labeled thiazolylazo-naphthol" Journal of Histochemistry and Cytochemistry. submitted.

Y. Sumi、T. Suzuki 和 K.T.