Role of histamine receptor ( <H_1> , <H_2> ) positive cells in contact hypersensitivity reaction (CS).

采用4-羟基-3-硝基苯乙酰（NP）诱导足垫肿胀的方法，研究组胺（H）对CS的免疫调节机制。CS在诱导期被H i. p. inj显著抑制，但在效应期不被抑制。这种抑制作用不受H-2基因的限制，并可<H_1>被受体<H_2>（R）拮抗剂消除.来自H注射小鼠的非尼龙柱贴壁细胞具有抑制NP免疫小鼠CS的能力。这些细胞膜表面有Thy1.2、Ly 1、Ly 2和I-J抗原。用<D_1>FITC标记的人血清白蛋白偶联H（FHH），用CoulterEPICS-C细胞分选仪对P388细胞进行HR研究。虽然FHH与P388<D_1>细胞膜结合，但H对FHH与细胞表面的结合无竞争性抑制作用，细胞浆中cAMP水平在正常范围内。

Immuno modurating mechanism of histamine (H) in CS was studied by footpad swelling induced with 4-hydroxy-3-nitrophenyl acetyl (NP). CS was markedly suppressed by H i.p.inj in induction phase but not in effector phase. This suppression was not ristricted by H-2 genes, and was eliminated by <H_1> and <H_2> receptor (R) antagonist. Non nylon column adherent cells from H injected mouse had capacity to suppress CS in NP immunized mice to which these cells were transfered. These cells had Thy1.2, Ly1, Ly2, and I-J antigens on membrance surface. Study on HR of P388 <D_1> cells was done by Coulter EPICS-C cell sorting using FITC labeled human serum albumin conjugated with H (FHH). Though FHH binded to P388 <D_1> cell membrane, H did not showed competitve inhibition of FHH binding to cell surface, and cyclic AMP level in these cells cytoplasma was in normal range.