Role of histamine receptor ( <H_1> , <H_2> ) positive cells in contact hypersensitivity reaction (CS).

组胺受体（<H_1>、<H_2>）阳性细胞在接触性超敏反应（CS）中的作用。

• 批准号: 60570298
• 负责人: TANI Kenji
• 金额: $ 1.15万
• 依托单位: YOKOHAMA CITY UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570298/
• 关键词: histamin; histamin receptor; contact hypersensitivity; nitrophenyl; P388【D_1】細胞

Immuno modurating mechanism of histamine (H) in CS was studied by footpad swelling induced with 4-hydroxy-3-nitrophenyl acetyl (NP). CS was markedly suppressed by H i.p.inj in induction phase but not in effector phase. This suppression was not ristricted by H-2 genes, and was eliminated by <H_1> and <H_2> receptor (R) antagonist. Non nylon column adherent cells from H injected mouse had capacity to suppress CS in NP immunized mice to which these cells were transfered. These cells had Thy1.2, Ly1, Ly2, and I-J antigens on membrance surface. Study on HR of P388 <D_1> cells was done by Coulter EPICS-C cell sorting using FITC labeled human serum albumin conjugated with H (FHH). Though FHH binded to P388 <D_1> cell membrane, H did not showed competitve inhibition of FHH binding to cell surface, and cyclic AMP level in these cells cytoplasma was in normal range.

用4-羟基-3-硝基苯乙酰（NP）致大鼠足跖肿胀法研究组胺（H）对CS的免疫调节作用。CS在诱导期被H i. p. inj显著抑制，但在效应期不被抑制。这种抑制不受H-2基因的限制，并<H_1>被受体<H_2>（R）拮抗剂消除。来自H注射小鼠的非尼龙柱贴壁细胞具有抑制NP免疫小鼠CS的能力。这些细胞膜表面有Thy1.2、Ly 1、Ly 2和I-J抗原。采用<D_1>Coulter EPICS-C细胞分选仪，使用异硫氰酸荧光素标记的人血清白蛋白偶联H（FHH）对P388细胞的HR进行研究。虽然FHH与P388<D_1>细胞膜结合，但H对FHH与细胞表面的结合无竞争性抑制作用，细胞浆中cAMP水平在正常范围内。

项目成果

Kenji Tani, Takao Okubo, Ronald P. Messner, Barry S. Handweger.: "Indirect <^(125)I-L> abeled Pratein A Assay for Monoclonal antibodies to Red Cell Surface Antigen" J. Immunol. Meth.

Kenji Tani、Takao Okubo、Ronald P. Messner、Barry S. Handweger.：“针对红细胞表面抗原单克隆抗体的间接 <^(125)I-L> abeled Pratein A 测定”J.Immunol。

Kenji Tani, Hisako Suzuki, Showhei Nagaoka, Keiichiro Matsunaga, Takao Okubo, Tsunemori Hiwatari, Masanori Toba.: "HIV-Antibody in Systemic Lupus Erythematosus Patients." Yokohama Med. Bull.37. 55-57 (1986)

Kenji Tani、Hisako Suzuki、Showhei Nagaoka、Keiichiro Matsunaga、Takao Okubo、Tsunemori Hiwatari、Masanori Toba.：“系统性红斑狼疮患者中的 HIV 抗体。”

Kenji Tani, Hiroshi Sakamoto, Kiyoshi Katoh, Keiichiro Matsunaga, Seiichi Yamada, Kenji Okuda, Barry S. Handwerger: "Establishment of Monoclonal Antibodies which Possess the Same Character of the National Occuring Thymocytotoxic Autoantibodies (NTA)." Imm

Kenji Tani、Hiroshi Sakamoto、Kiyoshi Katoh、Keiichiro Matsunaga、Seiichi Yamada、Kenji Okuda、Barry S. Handwerger：“建立与国家发生的胸腺细胞毒性自身抗体 (NTA) 具有相同特征的单克隆抗体。”

Sharad Lakhanpal, Kenji Tani, Yoshiaki Ishigatsubo, Kiyoshi Katoh, Takao Ohkubo, J.T.Lie: "Pathologic spectrum of Behcet syndrome : a review of Japanese autopsy registry date" Human Pathology. 16. 790-795 (1985)

Sharad Lakhanpal、Kenji Tani、Yoshiaki Ishigatsubo、Kiyoshi Katoh、Takao Ohkubo、J.T.Lie：“Behcet 综合征的病理谱：日本尸检登记日期的回顾”人类病理学。

Kiyoshi Katoh, Keiichiro Matsunaga, Jun Chiba, Yoshiaki Ishigatsubo, Kenji Tani: "A Case of Neuro-Vasculo-Eentero Behcet disease" J. Pheumatology. 12. 1186-1197 (1985)

Kiyoshi Katoh、Keiichiro Matsunaga、Jun Chiba、Yoshiaki Ishigatsubo、Kenji Tani：“神经血管肠白塞病一例”J. Pheumatology。