Studies on rheumatoid lung in murine model

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An animal model of collagen type II-specific monoclonal antibody-induced arthritis has been used for the evaluation of various therapeutic effects in joint inflammation of rheumatoid arthritis. We aimed to make a model for interstitial pneumonia of rheumatoid arthritis using this arthritis model. A commercially available antibody cocktail containing four monoclonal anti-type II collagen antibodies were injected into 6 to 8-week-old female DBA/1 female mice and 50 uL lipopolysaccharide was injected 3 days later. The clinical manifestations of RA were recorded and scored at 10 days (acute stage) and 21 days (chronic stage). Then the mice were sacrificed for histological analysis of interstitial pneumonia. We found that an animal model for interstitial pneumonia of rheumatoid arthritis was made by repeated injection with the Anthritogenic mAB Cocktail and lipopolysaccharide. A mild edema and slight infiltration of mononuclear cells were found in the alveolar wall treated with the Cocktail in mice in groups treated with 2mg x3 times and 4mg x once. The measurement of collagen amount and specific staining for collagen needs to clarify the fibrotic change in the lung. This model can be useful to investigate the mechanism of interstitial pneumonia due to rheumatoid arthritis and the evaluation of medications for intersititial pneumonia.