Changes of neuropeptide contents in rat brain after chronic interruption of dopaminergic transmission by administrations with haloperidol and 6-hydroxydopamine.

本文观察了长期氟哌啶醇（HAL）对大鼠脑内八肽胆囊收缩素（IR-CCK-8）和血浆催乳素（rPRL）的影响。HAL治疗通过皮下植入的渗透微型泵连续输注或每日腹膜内注射（间歇给药）进行。在14天间歇给药后，与对照组相比，血浆rPRL水平仍升高。与此相反，增加的rPRL水平在第一天的连续输注HAL消失迅速，被取代后，14天的rPRL的抑制释放。连续和间歇给药后，纹状体和中脑边缘区的IR-CCK 8含量均增加。与注射后1 h相比，在末次注射后24 h，这种变化更明显。结果提示，耐受性的形成是通过调节rPRL释放的结节-漏斗多巴胺系统实现的，内源性的rPRL释放可能是通过调节多巴胺的释放来实现的。 关于我们 本实验观察了侧脑室注射6-羟基多巴胺（6-OH-DA）对大鼠脑内CCK-8和生长抑素（SRIF）的影响。分别注射6-OH-DA和6-OH-DA后用帕吉林和地昔帕明处理，6天后用微波照射处死动物。如前所述，发现单独用6-OH-DA处理可耗尽所有测试区域中的DA和去甲肾上腺素浓度。6-OH-DA预处理或不预处理均可增加中脑、纹状体、额叶皮质和边缘前脑等DA神经元支配区域的IR-CCK 8和IR-SRIF含量。这些结果表明，内源性CCK-8和SRIF的控制下的DA和含有DA和CCK-8的神经元的人口是有限的，在相对较小的地区的大鼠脑和大量的CCK-8神经支配独立于DA神经元存在。少

Effects of long-term treatment with haloperidol (HAL) were studied on brain immunoreactive cholecystokinin octapeptide (IR-CCK8) and on plasma prolactin (rPRL) in the rat. HAL treatment was conducted with continuous infusion via osmotic minipump implanted subcutaneously or with daily intraperitoneal injection (intermittent administration). Plasma rPRL levels remained elevated compared to those in controls following 14-days intermittent administration. In contrast, increased rPRL levels at the first day of continuous infusion with HAL disappeared quickly, to be replaced after 14-days by an inhibited release of rPRL. IR-CCK8 contents in the striatum and mesolimbic areas were increased following both continuous and intermittent administration with HAL. This change was more obvious 24 h after the final injection as compared to 1h after the injection. The results suggest that the tolerance can be developed in the tubero-infundibular dopamine system regulating rPRL-release, and that endogeno … More us CCK-8 may have a role in the chronic effect of HAL.Effects of intracerebroventricular administration with 6-hydroxydopamine (6-OH-DA) were investigated on IR-CCK8 and somatostatin(SRIF) in the rat brain. The animals were injected with whether 6-OH-DA alone or 6-OH-DA following the treatment with pargyline and desipramine and were killed by microwave irradiation 6 days after the treatment. The treatment with 6-OH-DA alone was found to deplete both DA and norepinephrine concentrations in all areas tested as documented previously. 6-OH-DA administration with or without pretreatment elevated the contents of IR-CCK8 and IR-SRIF in several regions including midbrain, striatum, frontal cortex and limbic forebrain, where DA neurones innervate. These results suggest that endogenous CCK-8 and SRIF are under the control of DA and that the populations of neurones which contain both DA and CCK8 are limited in relatively small areas of the rat brain and substantial CCK-8 innervations exist independently from DA neurones. Less