Studies on mechanism of preretinal membrane formation and its surpression

1.培养研究：HAMF12和MEM的培养结果表明，HAMF12和MEM的培养基比RPMI1640更有利于培养皿上人眼视网膜前膜细胞的增殖。虽然大剂量的细胞被杀死，但小剂量的皂苷抑制了增殖细胞的运动行为。在这一系列研究中，PGE2、F2a和D2对细胞增殖的抑制作用不明显。在新生大鼠脑白质星形胶质细胞和视网膜色素上皮细胞的细胞培养研究中，超过0.5ug/ml的5FU和10ug/ml的地塞米松对细胞增殖有抑制作用。动物实验：在玻璃体液化引起的实验性视网膜脱离中，约有15%观察到玻璃体前膜。在实验性视网膜脱离眼玻璃体腔内注射上述抑制剂对玻璃体膜形成无明显影响。推测玻璃体膜形成的低发生率导致不能提供具有统计学意义的药物效果。用其新生儿脑白质来源的培养星形胶质细胞注射的雌性兔眼100%形成玻璃体膜。这些眼的膜主要由星形胶质细胞组成。

1. Culture studies.Tissue culture studies proved that the culture medium of HAMF12 and MEM were better than RPMI1640 in order to proliferate the component cells of preretinal membrane obtained from human eyes on the culture dishes. Moving behavior of proliferating cells was surpressed by saponin in small doses although the cells were killed in large doses. Surpressive effect on cellular proliferation by PGE2, F2a, and D2 was not apparent in this series of study. In cell culture studies of astrocytes and retinal pigment epithelial cells obtained from white matter of newborn rat brain, cellular proliferation was surpressed by administration in doses of more than 0.5 ug/ml of 5FU and 10 ug/ml of dexamethazon.2. Animal experiment.Preretinal membranes were observed in about 15% of experimental retinal detachment induced by vitreous liquification using hyarulonidase. The surpressive agents mentioned above injected into the vitreous cavity in experimental retinal detachment eyes did not reveal significant effects on vitreous membrane formation. It was speculated that low incidence of vitreous membrane formation resulted to fail in providing statistically significant effects of the agents. The vitreous membrane was produced in 100% of the female rabbit eyes which were injected by cultured astrocytes originated from the white matter of their newborn children's brain. The membranes in these eyes were mostly consisted of the astrocytes.