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Studies on mechanism of preretinal membrane formation and its surpression

视网膜前膜形成及其抑制机制的研究

基本信息

批准号：
60570829
负责人：
HORI Sadao
金额：
$ 1.02万
依托单位：
Tokyo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570829/
关键词：
Vitreous membrane retinal detachment astrocytes retinal pigment epithelial cells tissue culture surpression of cell movement 細胞増殖の抑制

项目摘要

1. Culture studies.Tissue culture studies proved that the culture medium of HAMF12 and MEM were better than RPMI1640 in order to proliferate the component cells of preretinal membrane obtained from human eyes on the culture dishes. Moving behavior of proliferating cells was surpressed by saponin in small doses although the cells were killed in large doses. Surpressive effect on cellular proliferation by PGE2, F2a, and D2 was not apparent in this series of study. In cell culture studies of astrocytes and retinal pigment epithelial cells obtained from white matter of newborn rat brain, cellular proliferation was surpressed by administration in doses of more than 0.5 ug/ml of 5FU and 10 ug/ml of dexamethazon.2. Animal experiment.Preretinal membranes were observed in about 15% of experimental retinal detachment induced by vitreous liquification using hyarulonidase. The surpressive agents mentioned above injected into the vitreous cavity in experimental retinal detachment eyes did not reveal significant effects on vitreous membrane formation. It was speculated that low incidence of vitreous membrane formation resulted to fail in providing statistically significant effects of the agents. The vitreous membrane was produced in 100% of the female rabbit eyes which were injected by cultured astrocytes originated from the white matter of their newborn children's brain. The membranes in these eyes were mostly consisted of the astrocytes.

1.组织培养研究表明，HAMF 12和MEM培养基比RPMI 1640培养基更有利于人视网膜前膜成分细胞在培养皿上的增殖。小剂量皂甙对增殖细胞的运动行为有抑制作用，大剂量皂甙对增殖细胞有杀伤作用。在该系列研究中，PGE 2、F2 a和D2对细胞增殖的抑制作用不明显。在从新生大鼠脑的白色物质获得的星形胶质细胞和视网膜色素上皮细胞的细胞培养研究中，细胞增殖被剂量大于0.5 μ g/ml的5 FU和10 μ g/ml的地塞米松抑制。动物实验：在玻璃体液化诱导的实验性视网膜脱离中，约15%的视网膜前膜被观察到。实验性视网膜脱离眼玻璃体腔内注射上述抑制剂对玻璃体膜形成无明显影响。据推测，玻璃体膜形成的发生率较低，导致无法提供药物的统计学显著性作用。将培养的新生儿脑白色组织来源的星形胶质细胞注入母兔眼，100%的母兔眼产生玻璃体膜。这些眼的膜主要由星形胶质细胞组成。