Mechanism of intraocular neovascularization and its regression

眼内新生血管形成及其消退机制

• 批准号: 02454411
• 负责人: HORI Sadao
• 金额: $ 3.46万
• 依托单位: Tokyo Women's Medical Collage
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454411/
• 关键词: intraocular neovascularization; regression; immuno-histochemistry; basic fibroblast growth factor; lysosomal enzyme; laser flare-cell meter; protein concentration; diabetic retinopathy; 免疫組識化学; 眼内血管新生; 角膜; 硝子体; 前房蛋白濃度; 組織化学; 塩基性線維芽細胞成長因子

Mechanism of intraocular neovascularization and its regression were investigated histochemically and immune-histochemically in the animal models. Intravitreal neovascularization was made in the eyes of monleys and pigmented rabbits by intravitreal implantation of basic fibroblast growth factor(b-FGF). Newly formed vessels were originated from the retina and choaroid in the monkey, and from the choroid in the rabbits.Corneal neovascularization was made by implantation of b-FGF in the corneal micropockets, and the newly formed vessels invaded from the lifflus towards the center of the cornea. Thelysoscmal enzymes were detected in the initial phase of neovascularization. Fibronectin was activated in the early phase of neovascualrization and vanished in the early phase of regression. Lamirline and type IV collagen were found to surround the new vessels and remained late in the course of regression. Factor VIII was detected on the endothelial cells of new vessels, and showed geographical stains in the regression phase. The protein concentration in the anterior chamber of diabetic patients calicurated by laser flare-cell meter in order to study a relationship between neovascualrization and intraocular leakage of plasma protein.The protein concentration in the anterior chamber increased as the stage of retinopathy advanced, and also increased after laser phtocoagulation treatment.

用组织化学和免疫组织化学方法研究了眼内新生血管形成及其消退的机制。用碱性成纤维细胞生长因子(b-FGF)玻璃体腔内植入兔眼玻璃体内新生血管。新生血管来源于猴的视网膜和脉络膜，兔的脉络膜，b-FGFs植入角膜微囊形成角膜新生血管，新生血管从角膜缘向角膜中心侵入。在新生血管形成的初期，检测到大量的体表酶。纤维连接蛋白在新生血管形成的早期被激活，在退化的早期消失。在新血管周围发现了层粘连和IV型胶原，并在消退过程中保持不变。在新生血管内皮细胞上检测到第VIII因子，并在退化期显示出地理染色。为探讨新生血管形成与血浆蛋白渗漏的关系，用激光散斑细胞仪测定了糖尿病患者前房蛋白浓度。随着视网膜病变的进展，前房蛋白浓度逐渐升高，激光光凝治疗后前房蛋白浓度也随之升高。

项目成果

Sadao Hori: "Pathophysiology of intraocular noevascularization." Acta Soc Ophthalmol Jpn. 92(12). 1103-1121 (1990)

Sadao Hori：“眼内新生血管的病理生理学。”

蔵並 貴子,木戸口 裕,北野 滋彦,堀 貞夫: "糖尿病網膜症に対する光凝固後早期における房水蛋白濃度の変化ーレ-ザ-フレアセルメ-タ-を用いてー" あたらしい眼科. 7. 161-165 (1990)

Takako Kuranami、Yutaka Kidoguchi、Shigehiko Kitano、Sadao Hori：“糖尿病视网膜病变光凝术后早期房水蛋白浓度的变化 - 使用激光耀斑细胞计”《新眼科学》7. 161-165 (1990)。

Takako Kuranami, Hiroshi Kidoguchi, Shigehiko Kitano, Sadao Hori: "Early changes in aqueous protein concentration measured by laser flare-cell meter after photocoagulation for diabetic retinopathy." Atarashii Ganka. 7(1). 161-165 (1990)

Takako Kuranami、Hiroshi Kidoguchi、Shigehiko Kitano、Sadao Hori：“糖尿病视网膜病变光凝术后通过激光耀斑细胞仪测量的水蛋白浓度的早期变化。”

堀 貞夫: "眼内血管新生の病態" 日本眼科学会雑誌. 94. 1103-1121 (1990)

Sadao Hori：“眼内新生血管的病理学”日本眼科学会杂志 94. 1103-1121 (1990)。

高橋 貴子,木戸口 裕,堀 貞夫: "糖尿病網膜症に対する光凝固長期後の房水蛋白濃度の変化" あたらしい眼科.

Takako Takahashi、Yutaka Kidoguchi、Sadao Hori：“糖尿病视网膜病变长期光凝后房水蛋白浓度的变化”新眼科。