Mechanism of intraocular neovascularization and its regression

用组织化学和免疫组织化学方法研究了眼内新生血管形成及其消退的机制。通过玻璃体内植入碱性成纤维细胞生长因子（b-FGF），在Monley和有色兔眼内形成玻璃体内新生血管。新生血管来源于猴视网膜和脉络膜，兔脉络膜，角膜微囊内植入b-FGF，新生血管从虹膜向角膜中央侵入。在新生血管形成的初始阶段检测到了溶酶体酶。纤维连接蛋白在新生血管形成早期被激活，在消退早期消失。层状和IV型胶原蛋白被发现包围的新血管，并保持在回归过程中的后期。在新生血管内皮细胞上检测到因子VIII，并在消退期显示地理染色。用激光闪光细胞仪测定糖尿病患者前房蛋白浓度，以探讨糖尿病视网膜新生血管形成与血浆蛋白渗漏的关系，发现糖尿病患者前房蛋白浓度随视网膜病变的进展而增加，激光光凝治疗后也增加。

Mechanism of intraocular neovascularization and its regression were investigated histochemically and immune-histochemically in the animal models. Intravitreal neovascularization was made in the eyes of monleys and pigmented rabbits by intravitreal implantation of basic fibroblast growth factor(b-FGF). Newly formed vessels were originated from the retina and choaroid in the monkey, and from the choroid in the rabbits.Corneal neovascularization was made by implantation of b-FGF in the corneal micropockets, and the newly formed vessels invaded from the lifflus towards the center of the cornea. Thelysoscmal enzymes were detected in the initial phase of neovascularization. Fibronectin was activated in the early phase of neovascualrization and vanished in the early phase of regression. Lamirline and type IV collagen were found to surround the new vessels and remained late in the course of regression. Factor VIII was detected on the endothelial cells of new vessels, and showed geographical stains in the regression phase. The protein concentration in the anterior chamber of diabetic patients calicurated by laser flare-cell meter in order to study a relationship between neovascualrization and intraocular leakage of plasma protein.The protein concentration in the anterior chamber increased as the stage of retinopathy advanced, and also increased after laser phtocoagulation treatment.