Electron microscopic studies on the cytoskeleton of taste buds.

本文研究了dd-小鼠轮廓乳头中由微管、5-nm细丝和10-nm细丝组成的味蕾细胞骨架。秋水仙碱诱导微管解聚后，Ⅰ型细胞分泌颗粒向顶面的运输被中断。单胺前体（5-HTP）和秋水仙素处理后，可延迟Ⅲ型荧光细胞的消失，并阻断Ⅲ型细胞核心囊泡向突触区的转运。2.味蕾顶端部分肌动蛋白免疫染色阳性。电子显微镜观察显示，在每个细胞的顶端过程和微绒毛的5纳米的细丝束。用重meromyoshin（HMM）孵育后，沿着这些细丝观察到特征性的箭头状结构。这表明5 nm纤维是由F作用组成的。味蕾细胞具有较低的密集聚集束的10纳米的细丝比周围的上皮细胞。III型细胞，但是，包含更密集的聚集束比I型或II型细胞。皮肤角蛋白的抗体染色了所有类型的味蕾细胞以及周围的上皮细胞。结果表明，构成味蕾的所有细胞类型都起源于味蕾周围的上皮细胞。一种45 kD角蛋白的单克隆抗体（PKK 3），通常位于简单的上皮细胞，染色味蕾细胞，但不周围的上皮细胞。

The cytoskeleton of taste buds composed of microtubules ,5-nm filaments, and 10-nm filaments was studied in the circumvallate papillae of dd-mice.1. After administration of colchicine which induces depolimerization of microtubules, the secretory granules of type-I cells were interrupted those transport to the apical surface. The treatment with both the monoamine precursor (5-HTP) and colchicine caused the delay of the disappearance of fluorescent type-III cells, and interrupted the transport of cored vesicles of type-III cells to synaptic areas.2.A positive reaction to immunostaining for actin were localized in the apical portion of the taste buds. Electron microscopic observations revealed a bundle of 5-nm filaments in the apical processes and microvilli in each cell. After incubation with heavy meromyoshin(HMM), characteristic arrowhead structures were observed along those filaments. It is concluded that 5-nm filaments are composed of F-action.3. Taste bud cells possessed less densely aggregated bundles of 10-nm filaments than the surrounding epithelial cells. Type-III cells, however, contained more densely aggregated bundles than type-I or -II cells. The antibodies to keratins form the skin stained all types of the taste bud cells as well as the surrounding epithelial cells. The result shows that all cell types comprising the taste buds originate from the epithelial cells surrounding the taste buds. A monoclonal antibody (PKK3) to a 45-kD keratin, which generally located in simple epithelia, stained taste bud cells, but not surrounding epithelial cells.