Electron microscopic studies on the cytoskeleton of taste buds.

研究了dd-小鼠周围乳头中由微管、5nm丝和10nm丝组成的味蕾细胞骨架。秋水仙碱诱导微管去聚后，i型细胞的分泌颗粒向根尖表面的运输中断。单胺前体（5-HTP）和秋水仙碱均可延缓荧光iii型细胞的消失，并阻断iii型细胞向突触区域的包囊运输。肌动蛋白免疫染色阳性反应集中在味蕾的顶端。电镜观察显示，每个细胞顶端突起有一束5纳米的细丝和微绒毛。与重肌球蛋白（HMM）孵育后，沿这些细丝观察到特征性的箭头结构。结果表明，5nm长丝由f -作用组成。味蕾细胞具有比周围上皮细胞更少的密集的10纳米丝束。然而，iii型细胞比i型或ii型细胞含有更密集的聚集束。角蛋白抗体形成皮肤染色的所有类型的味蕾细胞以及周围的上皮细胞。结果表明，构成味蕾的所有细胞类型都起源于味蕾周围的上皮细胞。一种针对45-kD角蛋白的单克隆抗体（PKK3），通常位于单层上皮，染色味蕾细胞，但不周围上皮细胞。

The cytoskeleton of taste buds composed of microtubules ,5-nm filaments, and 10-nm filaments was studied in the circumvallate papillae of dd-mice.1. After administration of colchicine which induces depolimerization of microtubules, the secretory granules of type-I cells were interrupted those transport to the apical surface. The treatment with both the monoamine precursor (5-HTP) and colchicine caused the delay of the disappearance of fluorescent type-III cells, and interrupted the transport of cored vesicles of type-III cells to synaptic areas.2.A positive reaction to immunostaining for actin were localized in the apical portion of the taste buds. Electron microscopic observations revealed a bundle of 5-nm filaments in the apical processes and microvilli in each cell. After incubation with heavy meromyoshin(HMM), characteristic arrowhead structures were observed along those filaments. It is concluded that 5-nm filaments are composed of F-action.3. Taste bud cells possessed less densely aggregated bundles of 10-nm filaments than the surrounding epithelial cells. Type-III cells, however, contained more densely aggregated bundles than type-I or -II cells. The antibodies to keratins form the skin stained all types of the taste bud cells as well as the surrounding epithelial cells. The result shows that all cell types comprising the taste buds originate from the epithelial cells surrounding the taste buds. A monoclonal antibody (PKK3) to a 45-kD keratin, which generally located in simple epithelia, stained taste bud cells, but not surrounding epithelial cells.