Induction of apoptosis of taste bud cells by denervation and administration of drugs

通过去神经和给药诱导味蕾细胞凋亡

• 批准号: 09671861
• 负责人: TAKEDA Masako
• 金额: $ 1.92万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671861/
• 关键词: taste bud; apoptosis; bcl-2; denervation; valinomycin; trophic factor; circumvallate papilla; カルシウムイオノフェア; Bcl-2; Bcl-x; 舌咽神経; コルヒチン

Gustatory nerve sectioning causes degeneration and disappearance of taste bud cells and after regeneration of the nerves, the taste bud cells regenerate. We recently (1996) reported that glossophaiyngeal nerve sectioning induced apoptosis of taste bud cells in the mouse circumvallate papillae. Thus, the trophic substance released from gustatory nerves, that causes differentiation and maintains taste bud cells, may have an inhibitory effect on apoptosis of taste bud cells.In this study we tried to examine the nature of the trophic substance. An immunohistochemical reaction for Bcl-2 and Bcl-x, which inhibit apoptosis, revealed positive staining of the epithelial cells in all layers of the epithelium and taste bud cells. Potassium ionophore valinomycin triggers an increase of the cytoplasinic calcium concentration. Valinomycin was injected in the mucosa near the circumvallate papillae in adult dd-mice tongues. Paraffin sections were cut and treated by method of DNA nick end labeling which detects apoptotic nuclei in situ. 20 and 24 h after injection of valinomycin, positive nuclei were scattered in the taste buds. The result shows that a rise in the intracellular content of calcium ion induces apoptosis of taste bud cells. Also we have established the method to inject the substances into the circumvallate papillae. We are examining whether apoptosis of the taste bud cells after nerve sectioning would be interfered by treatment of various kinds of growth factors such as BDNF and GDNF.

味觉神经切片使味蕾细胞变性消失，神经再生后，味蕾细胞再生。我们最近（1996）报道了舌咽神经切片诱导小鼠周围乳头味蕾细胞凋亡。由此可见，味觉神经释放的引起味蕾细胞分化和维持味蕾细胞的营养物质可能对味蕾细胞凋亡具有抑制作用。在这项研究中，我们试图检验营养物质的性质。抑制细胞凋亡的Bcl-2和Bcl-x的免疫组化反应显示，上皮细胞和味蕾细胞的各层上皮细胞均呈阳性染色。钾离子载体缬霉素引起细胞质钙浓度升高。在成年dd-小鼠舌周乳头附近粘膜注射缬霉素。石蜡切片用DNA缺口末端标记法原位检测凋亡细胞核。注射缬霉素后20、24 h，味蕾内可见分散阳性细胞核。结果表明，细胞内钙离子含量升高可诱导味蕾细胞凋亡。同时，我们也建立了将该物质注射到周围乳头内的方法。我们正在研究各种生长因子如BDNF和GDNF是否会干扰神经切片后味蕾细胞的凋亡。

项目成果

Yuko Suzuki: "Phagocytic cells in the taste buds of rat circumvallate papillae after denervation" Chemical Sences. 21. 476-476 (1996)

Yuko Suzuki：“去神经支配后大鼠味蕾中的吞噬细胞周围乳头”化学感觉。

武田正子: "神経切断後およびコルヒチン投与後の味蕾細胞のアポトーシス" 日本味と匂学会誌. 3・3. 556-559 (1996)

Masako Takeda：“神经切断和秋水仙碱给药后味蕾细胞的凋亡”日本味觉学会杂志 3・3（1996）。

Y. Suzuki: "Effect of denervation on lymphocytes and dendritic cells in the rat circumvallate and foliate papillae"Anatomy and Embryology. 196. 447-455 (1997)

Y. Suzuki：“去神经支配对大鼠外周和叶状乳头中淋巴细胞和树突状细胞的影响”解剖学和胚胎学。

Masako Takeda: "Expression of the neural cell adhesion molecule in mouse taste buds after denervation" Journal of Electron Microscopy. 48,1. 39-45 (1999)

Masako Takeda：“去神经支配后小鼠味蕾中神经细胞粘附分子的表达”电子显微镜杂志。

Yuko Suzuki, Masako Takeda, Nobuko Obara: "Effect of denervation on lymphocytes and dendritic cells in the rat circumvallate and foliate papillae."Anatomy and Embryology. 196. 447-455 (1997)

Yuko Suzuki、Masako Takeda、Nobuko Obara：“去神经支配对大鼠外周和叶状乳头中淋巴细胞和树突状细胞的影响。”解剖学和胚胎学。