Cell death of taste buds in the circumvallate papillae after section of the glossopharyngeal nerves.

舌咽神经切断后，周围乳头中的味蕾细胞死亡。

• 批准号: 07671979
• 负责人: TAKEDA Masako
• 金额: $ 1.41万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671979/
• 关键词: taste bud; denervation; apoptosis; colchicine; circumvallate papilla; epithelial cell; cell death; マウス; 舌咽神経

Apoptotic cells in the taste buds of mouse circumvallate papillae after the sectioning of bilateral glossopharyngeal nerves and the treatment with colchicine (inducer of microtubules depolimerization) were examined by the method of DNA nick-end labeling (TUNEL), together with standard electron microscopy. The taste buds decreased in number and size 3-11 days after denervation and disappeared at 11 days. The TUNEL method revealed only a few positively stained nucliei in normal taste buds but, in those of mice 1-5 days after denervation, the number of positive nuclei had increased to 3-5 times that of taste buds from normal mice. Electron-microscopic obsevation after denervation demonstrated taste bud cells containing codensed and fragmentary nuclei in a cytoplasm with increased density. The colchicine treatment after 15 hours and 1 day showed many TUNEL-positive nuclei and the condensed and fragmentary nuclei in the taste bud cells.The results show that taste bud cells under normal conditions die by apoptosis at the end of their life span, and that gustatory nerve sectioning and colchicine treatment cause apoptosis of taste bud cells.

用DNA缺口末端标记法（TUNEL）结合标准电镜观察了切断双侧舌咽神经和秋水仙碱（微管去聚合诱导剂）处理后小鼠轮廓乳头味蕾中凋亡细胞的变化。去神经后3-11天，味蕾数量和大小减少，11天后消失。TUNEL法显示正常小鼠味蕾中仅有少数阳性染色核，但去神经后1-5天，阳性核数增加到正常小鼠味蕾的3-5倍。电子显微镜观察表明，味蕾细胞在细胞质中含有密度增加的编码和碎片的核。秋水仙素处理15 h和1 d后，味蕾细胞中出现大量TUNEL阳性细胞核，细胞核浓缩、破碎，表明正常条件下的味蕾细胞在其寿命末期以凋亡方式死亡，而切断味觉神经和秋水仙素处理可引起味蕾细胞凋亡。

项目成果

Masako Takeda: "Apoptosis in mouse taste buds after denervation." Cell and Tissue Research. (印刷中). (1996)

Masako Takeda：“去神经后小鼠味蕾的细胞凋亡”（细胞和组织研究）（1996 年）。

武田正子: "神経切除後およびコルヒチン投与後の味蕾細胞のアポトーシス" 日本味と勾学会誌. 3・3. 556-559 (1996)

Masako Takeda：“神经切除和秋水仙碱给药后味蕾细胞的凋亡”Nippon Aji to Kogakkai Journal 3・3（1996）。

Masako TAKEDA et al.: "Apoptosis in mouse taste buds after denervation." Cell and Tissue Research. 186-1. 55-62 (1996)

Masako TAKEDA 等人：“去神经后小鼠味蕾的细胞凋亡。”

Masako TAKEDA et al.: "N-CAM of taste buds after section of the glossopharyngeal nerves." Journal of Electron Microscopy. 44-5. 421 (1995)

Masako TAKEDA 等人：“舌咽神经切片后味蕾的 N-CAM。”

Masako Takeda: "Apoptosis in mouse taste buds after denervation." Cell and Tissue Research. 286・1. 55-62 (1996)

Masako Takeda：“去神经后小鼠味蕾的细胞凋亡。”286・1（1996）。