Studies on <alpha> -Helixal Hydrophobic Peptide Rods in Biological Membranes

生物膜中<α>-螺旋疏水肽棒的研究

• 批准号: 60580124
• 负责人: KATAKAI Ryoichi
• 金额: $ 0.83万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60580124/
• 关键词: Hydrphobic peptides; <alpha> -Helix; Liposomes; 膜厚

This research project has been carried out to find experimental evidences to the predicted transmembrane peptide toporaphy that hydrophobic <alpha> -helical moieties of the membrane proteins are in the membranes. For this purpose, hydrophobic <alpha> -helical oligopeptides soluble in lipophilic solvents suitable for the preparation of liposomes from lecitines Nps- <(Met-Met-Leu)_n> -OEt (n=6-10) were synthesized , and the liposomes consisting of these peptides and egg yolk lecitine and other lecitines were prepared by sonication method. Analyses of the content and conformation of the peptides incorporated into the liposomes showed that the peptides assume the <alpha> -helical conformation in the membranes and that the <alpha> -helical rods having the length comparable to the thickness of the membranes are best incorporated into the membranes. This result supports the predicted transmembrane hydrophobic peptide topography.

本研究旨在为预测的跨膜肽拓扑学寻找实验证据，即<alpha>膜蛋白的疏水螺旋部分存在于膜中。为此，以<alpha>卵磷脂Nps-（Met-Met-Leu）_n&gt; -OEt（n=6-10）为原料，合成了可溶于亲脂溶剂的疏水螺旋寡肽，并将其与蛋黄卵磷脂及其它卵磷脂用超声法制备了脂质体。掺入脂质体中的肽的含量和构象的分析表明，肽<alpha>在膜中呈现-螺旋构象，并且具有<alpha>与膜的厚度相当的长度的-螺旋杆最好掺入膜中。该结果支持预测的跨膜疏水肽拓扑。

项目成果

Ryoichi Katakai;Yasuko Iizuka: Biochemistry.

片井亮一；饭冢靖子：生物化学。

Ryoichi Katakai: " <alpha> -Helical Oligopeptides with Hydrophobic Side Chains Soluble in Lipophilic Solvents" BIOPOLYMERS. 25. 1-4 (1986)

Ryoichi Katakai：“具有疏水性侧链的<α>-螺旋寡肽可溶于亲脂性溶剂”生物聚合物。

Ryoichi Katakai: Biopolymers. 25. 1-4 (1986)

片井良一：生物聚合物。

Ryoichi Katakai and Yasuko Iizuka: "Experimental Evidences for Predicted Transmembrane Peptide Topography:Incorporation of Hydrophobic Peptide <alpha> -Helical Rods into the Membranes" BIOCHEMISTRY. submitted

Ryoichi Katakai 和 Yasuko Iizuka：“预测跨膜肽形貌的实验证据：将疏水性肽 <α> -螺旋杆纳入膜中”生物化学。