Studies on the unclassified and undescribed plant viruses inJapan

日本未分类和未描述的植物病毒的研究

• 批准号: 61304018
• 负责人: YAMASHITA Shuichi
• 金额: $ 5.18万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61304018/
• 关键词: plant viruses; new virues; Japanese viruses; ウイルスの同定分類

I. Particle morphology, physiochemical properties and intracellular appearance were investigated in some unclassified viruses from fields and inoculations. Characters of purified preparation and possibility of aphid transmission suggested that GLRV may be a member of closterovirus. RSV with unique natures such as vector tramsmission or morphology were classified as a type virus of tenuivirus established newly in ICTV. CuYV suggested to be classified a new group from its whitefly-transmission and phloem-limited distribution. Non-enveloped viruses such as OFV and CYMV, etc. couldn't found their vectors, may be also classified into new group, because of many natures. GAV and GSV of phloem-limitted viruses were purified from host grapevine and the latter was transmitted by a planthopper. These viruses were intersted in toxonomy. BBWV, a type virus of Fabavirus was grouped two serological types (strains) among many isolates. 2. Physiochemical properties, structural components, genome analys … More is and translation using SBWMV and BNYVV, members of furovirus of fungal-borne rod-shaped viruses, were investigated in purified preparations, and then their classificational characters were obtained from the results. 3. In seed-borne temperate viruses, physiochemical properties, genome analysis and serological properties were studied among many viuses. Some of them were also compaired with ones of European viruses. These viruses have been classified into phytocrypticvirus established newly ICTV. 4. Viruses in quarantine plants of economic plants such as fruit trees and flowers were screened by inoculation tests, purification, electron microscopy and antisera. Many important viruses of nepovirus, ilarvirus and closferovirus were found. CTLV and tulip-associated filamentous virus were interested in toxonomy. 5. TSV and LBVV of quitely undescribed rod-shaped viruses containing double-stranded RNA were analized on some virological natures, and LBVV was compaired with foreign isolates. These two viruses should be classified into a new virus group from their characteristic natures. Less

I.对田间和接种的未分类病毒的颗粒形态、理化性质和胞内外观进行了研究。从纯化物的特性和蚜虫传毒的可能性来看，GLRV可能是一种梭状病毒。RSV是在ICTV中新建立的细小病毒属的一个类型病毒，具有独特的媒介传播或形态学特性。根据CuYV的粉虱传播和韧皮部局限性分布，建议将其划分为一个新的类群。无囊膜病毒如OFV、CYMV等由于其自身的特性，目前还没有找到它们的载体，也可能被归入新的一类。从寄主葡萄藤上分离到韧皮部局限病毒的GAV和GSV，后者由稻飞虱传播。对这些病毒进行了毒理学研究。BBWV是Fabavirus的一种类型病毒，在许多分离物中分为两个血清型（株）。2.理化性质、结构成分、基因组分析 ...更多信息 对分离纯化的杆状病毒中糠病毒属成员SBWMV和BNYVV进行了序列分析和翻译，并根据结果确定了它们的分类特征。3.对种子传温性病毒的理化特性、基因组分析和血清学特性进行了研究。其中一些还与欧洲病毒进行了比较。这些病毒已被归类为ICTV新建立的植物隐病毒。4.通过接种试验、病毒分离纯化、电镜观察和抗血清等方法，对果树、花卉等经济植物检疫植物中的病毒进行了筛选。发现了许多重要的病毒，包括线虫传痘病毒、稻瘟病毒和封闭病毒，其中CTLV和郁金香相关丝状病毒引起了人们的兴趣。5.对尚未报道的含双链RNA杆状病毒TSV和LBVV进行了病毒学性质分析，并与国外分离物进行了比较。从这两种病毒的特征性质来看，应将其归为一个新的病毒组。少

项目成果

山下修一 他: Ann.Phytopath.Soc.Japan. 53. (1987)

Shuichi Yamashita 等人：Ann.Phytopath.Soc.Japan 53。（1987）

白子幸男 他: Ann.Phytopath.Soc.Japan. 52. 453-459 (1986)

Yukio Shirako 等人：Ann.Phytopath.Soc.Japan 52. 453-459 (1986)。

桑田茂 他: 日植病報. 52. 554 (1986)

Shigeru Kuwata 等人：日本植物病害报告 52. 554 (1986)。

山下修一 日高他編: "微生物学辞典" 技報堂, (1987)

山下修一、日高等编：《微生物学辞典》Gihodo，（1987）

白子幸男 ら: 日植病報. 53. 430 (1987)

Yukio Shirako 等人：日本植物病害报告 53. 430 (1987)。