Cell-surface glycoconjugates as indicators of cell functions

细胞表面糖偶联物的结构和定位随着细胞功能的改变以及细胞的分化而非常敏感地发生变化。如果准确地阐明这些结构变化对细胞功能和分化阶段变化的特征，细胞表面糖结合物的糖链结构是反映细胞功能或分化阶段的良好指标。在这项研究中，发展了各种新的方法来阐明细胞-表面糖共轭的极少量糖链的结构。用这些方法阐明了T细胞各功能亚群、不同分化阶段的鸡胚和病变组织的细胞表面糖蛋白的结构特征。这些发现可以有效地应用于细胞功能和分化阶段的研究。此外，本研究还分析了糖共轭糖链结构变化的主导因素。

Structures and localization of cell-surface glycoconjugates are known to change very sensitively upon the change of cell functions and also during the differentiation of cells. If the characteristics of these structural changes on the change of cell functions and differentiation stages are precisely elucidated, the structure of the sugar chain of cell-surface glycoconjugate is a good indicator of the cell function or the differentiation stage. In this study, various novel methods for the elucidation of the structure of a very tiny amount of the sugar chain of cell-surface glycoconjugate were developed. Using these mothods, the structural characteristics of cell surface glycoproteins of various functional subsets of T cells, those of chicken embryos in various differentiation stages and those of diseased tissues were elucidated. These findings can be usefully applied to the persumption of cell functions and differentiation stages. Furthermore, the gones dominating structural changes of sugar chains of glycoconjugates were analyzed in this study.