Cell-surface glycoconjugates as indicators of cell functions

细胞表面糖复合物作为细胞功能的指标

• 批准号: 61304063
• 负责人: OSAWA Toshiaki
• 金额: $ 7.49万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61304063/
• 关键词: Glycoconjugate; lectin; glycoprotein; glycolipid; proteoglycan; 分化; 糖たんぱん質; 免疫細胞; グリコシルトランフェラーゼ; グリコシルトランスフェラーゼ

Structures and localization of cell-surface glycoconjugates are known to change very sensitively upon the change of cell functions and also during the differentiation of cells. If the characteristics of these structural changes on the change of cell functions and differentiation stages are precisely elucidated, the structure of the sugar chain of cell-surface glycoconjugate is a good indicator of the cell function or the differentiation stage. In this study, various novel methods for the elucidation of the structure of a very tiny amount of the sugar chain of cell-surface glycoconjugate were developed. Using these mothods, the structural characteristics of cell surface glycoproteins of various functional subsets of T cells, those of chicken embryos in various differentiation stages and those of diseased tissues were elucidated. These findings can be usefully applied to the persumption of cell functions and differentiation stages. Furthermore, the gones dominating structural changes of sugar chains of glycoconjugates were analyzed in this study.

已知细胞表面糖缀合物的结构和定位随着细胞功能的变化以及细胞分化过程中的变化而非常敏感。如果能够准确阐明这些结构变化对细胞功能和分化阶段变化的特征，细胞表面糖复合物的糖链结构是细胞功能或分化阶段的良好指标。在这项研究中，开发了多种新方法来阐明细胞表面糖复合物的极微量糖链的结构。利用这些方法，阐明了T细胞各功能亚群的细胞表面糖蛋白、不同分化阶段鸡胚的细胞表面糖蛋白以及病变组织的细胞表面糖蛋白的结构特征。这些发现可有效应用于细胞功能和分化阶段的推测。此外，本研究还分析了糖复合物糖链结构变化的主导因素。

项目成果

N.Tomiya: Anal.Biochem.171. 73-93 (1988)

N.Tomiya：Anal.Biochem.171。

S.Hase: J.Chromatogr.434. 51-56 (1988)

S.Hase：J.Chromatogr.434。

H.Imai: Molecular Immunol.25. 419-428 (1988)

H.Imai：分子免疫学25。

S.Koyama: FEBS Letter. 209. 265-268 (1986)

S.Koyama：FEBS 信。

S.Sueyoshi: Carbohydrate Research. (1987)

S.Sueyoshi：碳水化合物研究。