Molecular mechanism of sodium Pump
钠泵的分子机制
基本信息
- 批准号:61304065
- 负责人:
- 金额:$ 7.04万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Co-operative Research (A)
- 财政年份:1986
- 资助国家:日本
- 起止时间:1986 至 1987
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1.Molecular Structure : Primary structure of and chains from torpedo and human Na^+,K^+-ATPase were deduced from the cloned cDNA's. Structures of three isoforms, , (+) and III were also deduced in rat brain. mRNA's of and chains synthesized from the cDNA's were injected into Xenopus oocytes, and the translated products could exhibit the Na^+,K^+-ATPase activity and the Na pump activity. Although each translated and chain independently had an incorporation ability to the membrane, co-existence of with was necessary for the expression of the physiological function with Na^+,K^+-ATPase by lowangle laser light scattering photometry coupled with HPLC, the change of protomer interaction was proved to corelate with the conformational change of the enzyme during turnover.2.Enzyme Reaction Mechanism : Extrinsic fluorescence of BIPM, FITC, IAF and DACM, intrinsic fluorescence of Trp, light scattering and ^<42>K^+ binding were measured in each of the reaction intermediates, and the conformational … More change of the enzyme was analyzed with the above parameters. Inhibition by amine compounds was investigated to know the nature of cation sites. 3.Transport Function : H^+ transport could be substituded in Na^+ transport by Na pump with the reconstructed vesicles. Insulin increased Na^+ eflux with frog skeletal muscle, and the increase was concluded to be due to the increase in the number of Na^+,K^+-ATPase molecule which was translocated to the plasma membrane from the inside of cell. During maturation of the primary cultured neurons from rat, (+) enzyme was induced, and the (+) pump activity functioned preferentially than the pump activity. mRNA's for , (+) and III were determined with cDNA probes in rat brain, heart and kidney, and the changes in the content were examined in the course of cell culture. Presence of three kind of cation co-transport systems, i.e., Na^+-dependent, H^+ dependent, and Na^+ or H^+-dependent system, was demonstrated in the brush border membrane of intestin. Less
1.分子结构:从克隆的cDNA推导出鱼雷和人Na^+,K^+-ATP酶的一级结构和链。在大鼠脑内还推测了三种异构体的结构。将cDNA的mRNA及其合成的cDNA链注入非洲爪蟾卵母细胞,翻译产物可显示Na^+,K^+-ATP酶活性和Na泵活性。虽然每个翻译链和链都独立地具有与膜结合的能力,但通过低角激光散射光度法结合HPLC,证明了与Na^+,K^+-ATP酶的生理功能的表达需要与共存,并且在周转过程中,原聚体相互作用的变化与酶的构象变化相关。在每种反应中间体中测量了BIPM、FITC、IAF和DACM的外荧光,Trp的内荧光,光散射和^<42>K^+结合,以及构象 ...更多信息 用上述参数分析酶的变化。研究了胺类化合物的抑制作用,以了解阳离子位点的性质。3.转运功能:重组囊泡可代替Na^+泵转运H^+。胰岛素使蛙骨骼肌Na^+流出量增加,其原因可能是Na^+,K^+-ATP酶分子数量增加,从细胞内转位到质膜上。在原代培养的大鼠神经元成熟过程中,(+)酶被诱导,(+)泵活性优先于泵活性发挥作用。用cDNA探针测定了大鼠脑、心和肾中的β-D-、β-D-存在三种阳离子共转运系统,即,小肠刷状缘膜存在Na^+依赖、H^+依赖、Na^+或H^+依赖系统。少
项目成果
期刊论文数量(65)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nobuo Inoue: "Nerve growth factor induces Na^+,K^+-ATPase in a nerve cell line." J. Neurochem.50. 230-236 (1988)
Nobuo Inoue:“神经生长因子在神经细胞系中诱导 Na^ ,K^ -ATP 酶。”
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- 影响因子:0
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- 通讯作者:
Momohiko Nagai: "Identification of N-[p-(2-benzimidazoyl)phenyl]maleimide-modified residue participating in dynamic fluorescence changes accompanying Na^+,K^+-dependent ATP hydrolysis." J. Biol. Chem.261. 13197-13202 (1986)
Momohiko Nagai:“鉴定 N-[对-(2-苯并咪唑基)苯基]马来酰亚胺修饰残基参与伴随 Na^ 、K^ 依赖性 ATP 水解的动态荧光变化。”
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- 影响因子:0
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Kazuya Taniguchi: "Acceleration of the rate of fluorescence change by high concentration of ATP under the condition of accumulation of ADP-sensitive phosphoenzyme in Na^+,K^+-ATPase." J. Biochem.100. 1231-1239 (1986)
Kazuya Taniguchi:“在 Na^ ,K^ -ATPase 中 ADP 敏感磷酸酶积累的条件下,高浓度 ATP 加速荧光变化速率。”
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- 影响因子:0
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Yoshio Maruyama: Pfl【u!¨】gers Arch.406. 563-567 (1986)
Yoshio Maruyama:Pfl【u!ě】gers Arch.406(1986)。
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- 影响因子:0
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MATSUI Hideo的其他文献
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21310076 - 财政年份:2009
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