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Determination of Chromasomal localization of Tumor Suppressing Genes Associted with Oncogenesis of Choriocarcinoma

绒毛膜癌发生相关抑癌基因染色体定位的测定

基本信息

批准号：
04670989
负责人：
MATSUI Hideo
金额：
$ 1.41万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670989/
关键词：
Choriocarcinoma Hydatidiform mole Tumor suppressing gene DNA polymorphism PCR

项目摘要

1. The pregnancies responsible for 6 choriocarcinoma cases were successfully indenrified by DNA polymarphism analysis using PCR method. Choriocarcinoma DNA samples were extracted from formalin-fixed, paraffin-embedded tissue(2 cases), tumors implanted on nude mice(2 cases), fresh poerational specimen(1 case). Four out of 6 choriocarcinonmas were determinded to derive from hydatidiform moles. Ouranalysis revealed an interesting case which was not derived from the last normal pregnancy, but a preceeding hydatidform mole. One out of these 4 moles has been already shown to exhibit chromosomal heterogenity. Addidionally, our analysis was cofirmed to ve useful for the differntial diagnosis of twin pregnancies with a mormal pregnancy and hydatidiform mole. One out of4 cases caused a choriocarcinoma, in which the hydatidiform mole showed chrmosomal heterogenity. 2. TheDNA polymorphisims, were demonstrated to ve remarkably informative and easily detected. Therfore, we amplified DNAsequences showing this type of polymorphism by PCR rechnique and anallysed differences in band pattern betweeneach sample. In paticular, microsatellites derived form fmre than 3 base-pair repeats were most useful for our analysis in terms of 1) simple detection system consisting ofPCR, electrophoresis on polyacrylamide gel, and silver staining and 2) wide and multiple distribution on each chromosome. 3. Whithin a given study period we failed to collect enough numbers of choriocarcinoma samples for satistical analysis and to determine the chromosomes showing constantly homozygosity in herero-moles. This wa arised form the rapid decrease in morbidity of the disease inself and the cases treated with operation. It is shus snceassary collect samples in collabration with other organizations.

1. 采用PCR方法对6例绒毛膜癌患者的妊娠情况进行了DNA多态性分析。绒毛膜癌DNA分别取自福尔马林固定、石蜡包埋组织（2例）、裸鼠移植肿瘤（2例）、新鲜手术标本（1例）。6个绒毛膜癌中有4个被确定源自包膜样痣。我们的分析揭示了一个有趣的病例，它不是源于上一次正常妊娠，而是先前的葡萄胎。这4个痣中有一个已经显示出染色体异质性。此外，我们的分析被证实是有用的鉴别诊断双胎妊娠与正常妊娠和葡萄胎。1 / 4的病例引起绒毛膜癌，其中葡萄胎表现出染色体异质性。2. dna多态性被证明具有显著的信息性和易于检测。因此，我们用PCR技术扩增了显示这种多态性的dna序列，并分析了每个样本之间的条带模式差异。特别是，从超过3个碱基对重复序列衍生的微卫星对我们的分析最有用，因为1)由pcr、聚丙烯酰胺凝胶电泳和银染色组成的简单检测系统；2)每条染色体上的广泛和多重分布。3. 在给定的研究期间，我们未能收集足够数量的绒毛膜癌样本进行满意的分析，并确定在hero -mole中显示持续纯合的染色体。这是由于该病本身的发病率和手术治疗病例的迅速下降。因此，有必要与其他组织合作收集样本。