Studies on the mechanisms of specific induction of avenalumin, phytoalexin of oats

燕麦抗毒素（Avenalumin）的诱导机制研究中，发现<alpha>精氨酸脱羧酶的特异性抑制剂DL-二氟甲基精氨酸（DL-difluoromethylarginine）和腐胺、尸胺等阳离子二胺化合物可抑制燕麦抗毒素的产生。因此，多胺代谢和燕麦铝蛋白的积累之间的关系进行了研究，在燕麦组织中响应不兼容的锈病感染，维克多林作为激发剂，和渗透压休克的甘露醇soluble。腐胺明显增加平行的积累燕麦铝引起的压力。1，3-丙二胺和精胺在抗锈侵染的表达期也略有增加。精氨酸<alpha>脱羧酶抑制剂DL- -二氟甲基精氨酸可抑制应激引起的大鼠Pu 关于我们 三胺合成和avenalumin积累，而DL-<alpha>-二氟甲基鸟氨酸，一种鸟氨酸脱羧酶的抑制剂，没有，表明前一种途径通过精氨酸激活。另一个有趣的事实是，通过外源施用二胺、腐胺和尸胺处理，防止了胁迫诱导的燕麦铝蛋白在燕麦叶片中的积累。腐胺合成的内部上升也受到抑制的聚阳离子化合物。通过电泳分析Victorin处理叶片中的蛋白质，发现了与燕麦铝和多胺代谢平行的跨膜水平的激活。诱导子victorin处理后，叶片中的一条蛋白质带明显增强，并出现一条新的蛋白质带。燕麦铝积累和多胺合成的激活是由对各种胁迫的刺激识别触发的。表明精氨酸脱羧酶在调节多胺代谢和燕麦铝蛋白合成中起重要作用。对酶诱导的深入研究对于了解燕麦铝蛋白生产的机制是必要的

During the study of induction mechanisms of avenalumin, phytoalexin of oats, in response to pathogen infection, it happened to know that the production of avenalumin was prevented by DL-<alpha>-difluoromethylarginine, a specific inhibitor of arginine decarboxylase and the cationic diamine compounds such as putrescine and cadaverine Polyamine metabolism is known to play an important role in the metabolic shift of plant cells under various stresses. Thus, relationship between polyamine metabolism and the accumulation of avenalumin was examined in oat tissues responding to incompatible rust infection, victorin as elicitro, and osmotic shock in mannitol solutin. Putrescine apparently increased in parallel with the accumulation of avenalumin elicited by the stresses. 1,3-diaminopropane and spermine also slightly increased in the expression phase of resistance to rust infection. DL-<alpha>-difluoromethylarginine, an inhibitor of arginine decarboxylase, prevented the spress-induced riss in pu … More trescine synthesis and avenalumin accumulation, whereas DL-<alpha>-diffluoromethylornithine, an inhibitor of ornithine decarboxylase, did not, indicating the activation of the former pathway via arginine. Another interesting fact was the prevention of the stress-inuced accumulation of avenalumin in oat leaves by the treatment with exogenouslyapplied diamines, putrescine and cadaverine. Internal rise in putrescine synthesis was also inhibited by the polycationic compounds. Electroophoretic analysis of proteins in victorin-treated leaves was carried out to find out the activation at tlanslational level in parallel with avenalumin and polyamine metabolisms. A band of protein was greatly intensified and a new band was found in the leaves treated with the elicitor, victorin. Activation of avenalumin accumulation and polyamine syntheses is triggered by irritative recognition to various stresses. It was indicated that arginine decarboxylase played an important role to regulate the polyamine metabolism as well as avenalumin production. Intense study of the enzyme induction is necessary to understand the mechanisms of avenalumin production Less