Identification and cloning of genes conditioning species-specific parasitism of Magnaporthe oryzae

调节稻瘟病物种特异性寄生的基因的鉴定和克隆

• 批准号: 14206006
• 负责人: MAYAMA Shigeyuki
• 金额: $ 30.2万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14206006/
• 关键词: Magnaporthe; Pyricularia; Mgnaporthe; 非病原力遺伝子; Magnaporthe oryzae; Magnaporthe grisea; Pyricularia oryzae; Pyricularia grisea; 種特異的寄生性; 遺伝子対遺伝子説; コムギ; avirulence gene; blast fungus; AFLP; bulked segregant analysis

To clarify genetic mechanisms of plant-species specificity of Magnaporthe oryzae, we crossed a Triticum isolate with a Setaria isolate, an Avena isolate, and an Oryza isolate. Using the F_1 progenies derived from these crosses, we identified PWT1, PWT2 (conditioning avirulence of the Setaria isolate on wheat), PWT3, PWT4 (conditioning avirulence of the Avena isolate on wheat), PWT5 (conditioning avirulence of the Oryza isolate on wheat), PFM1, PFM2 (conditioning avirulence of the Triticum isolate on foxtail millet), and PAT1 (conditioning avirulence of the Triticum isolate on oat). We also identified a resistance gene corresponding to PWT4, and designated it as Rwt4. Rwt4 corresponded to PWT4 in a one-to-one manner, suggesting that the plant species specificity of the Triticum/Avena isolates on wheat is conditioned by gene-for-gene interactions. From these avirulence genes, PWT1, PWT4, and PAT1 were chosen as targets for molecular cloning. Using bulked segregant analysis, we found molecular markers that are closely linked to PWT1, PWT4 and PAT1.

为了阐明 Magnaporthe oryzae 植物物种特异性的遗传机制，我们将小麦分离株与狗尾草分离株、燕麦分离株和稻分离株杂交。使用这些杂交衍生的 F_1 后代，我们鉴定了 PWT1、PWT2（条件化狗尾草分离株对小麦的无毒力）、PWT3、PWT4（条件化燕麦分离株对小麦的无毒力）、PWT5（条件化稻分离株对小麦的无毒力）、PFM1、PFM2（条件化小麦分离株的无毒力） 谷子上的分离株）和 PAT1（燕麦上小麦分离株的无毒调节）。我们还鉴定了一个与PWT4相对应的抗性基因，并将其命名为Rwt4。 Rwt4 以一对一的方式对应于 PWT4，这表明小麦/燕麦分离株的植物物种特异性是由基因间相互作用决定的。从这些无毒基因中，选择 PWT1、PWT4 和 PAT1 作为分子克隆的靶标。通过批量分离分析，我们发现了与 PWT1、PWT4 和 PAT1 密切相关的分子标记。

项目成果

Significance of the PWT4-Rwt4 interaction in the species specificity of Avena isolated of Magnaporthe oryzae on wheat.

PWT4-Rwt4 相互作用对小麦稻瘟病菌分离燕麦物种特异性的意义。

• 发表时间: 2005
• 期刊: Journal of General Plant Pathology (印刷中)
• 作者: Nishio T;Mukai Y;Oosawa R;Kusaba M;Toriyama K;Kenji HIRATA
• 通讯作者: Kenji HIRATA

Significance of the PWT4-Rwt4 interaction in the species specificity of Avena isolates of Magnaporthe oryzae on wheat.

PWT4-Rwt4 相互作用在小麦稻瘟病燕麦分离株物种特异性中的意义。

• 发表时间: 2005
• 期刊: J.Gen.Plant Pathol (in press)
• 作者: Hirata;K.;Tosa;Y;Nakayashiki;H.;Mayama;S.
• 通讯作者: S.

Oh, H.S.: "Characterization of an Avena isolate of Magnaporthe grisea and identification of a locus conditioning its specificity on oat"Can. J. Bot.. 80. 1088-1095 (2002)

哦，H.S.：“稻瘟病菌燕麦分离株的表征以及调节其在燕麦上的特异性的基因座的鉴定”可以。

A Gene-for-Gene Relationship Underlying the Species-Specific Parasitism of Avena/Triticum Isolates of Magnaporthe grisea on Wheat Cultivars.

• DOI: 10.1094/phyto.2002.92.11.1182
• 发表时间: 2002-11
• 期刊: Phytopathology
• 影响因子: 3.2
• 作者: N. Takabayashi;Y. Tosa;H. Oh;S. Mayama

Tosa, Y. et al.: "Genetic constitution and pathogenicity of Lolium isolates of Magnaporthe oryzae in comparison with host species-specific pathotypes of the blast fungus"Phytopathology. 第94巻(in press). (2004)

Tosa, Y. 等人：“稻瘟病菌黑麦草分离株的遗传组成和致病性与稻瘟病菌宿主物种特异性致病型的比较”《植物病理学》第 94 卷（出版中）。