Rapid identification of races and species-specific pathotypes using DNA-fingerprinting

使用 DNA 指纹快速鉴定种族和物种特异性致病型

• 批准号: 10556011
• 负责人: MAYAMA Shigeyuki
• 金额: $ 5.57万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10556011/
• 关键词: blast fungus; Magnaporthe grisea; race; DNA-fingerprinting

DNA fingerprinting with miltilocus probes, MAGGY and MGR586, were conducted to investigate the population structure of the rice blast fungus, Magnaporthe grisea in Japan. Analyses of ca. 300 isolates revealed that two discrete lineages exist in the current rice blast population in Japan. They were designated as JL1 and JL2. There two lineages corresponded to two of the five lineages previously detected in collections made before 1960, suggesting that the structure of the rice blast population in Japan had drastically changed during 1960-1976. Rice blast population in Vietnam were also subjected to DNA fingerprinting with MAGGY probe and the pathotype analysis. These analyses revealed that the blast population in the northern region of Vietnam is genetically more diverse than that in the southern region. In both populations no relationships were detected between lineages and races, suggesting that the "lineage exclusion hypothesis" is not applicable to those populations.DNA fingerprinting experiments were carried out for establishing a system of DNA-diagnosis of six host-specific subgroups of M. grisea ; Oryza isolates, Triticum isolates, Panicum isolates, Setaria isolates, Eleusine isolates, and Digitaria isolates. We propose the following formulas for distinguishing those subgroups.(1)HindIII+MGR583 : to identify each group(2)SalI+MAGGY : to identify the Oryza and the Setaria isolates(3)EcoRV+MGR583 : to identify the Triticum isolates(4)HindIII+Pot2 : to identify the Digitaria isolates

利用多位点探针Maggy和MGR586对日本稻瘟病菌的种群结构进行了研究。对大约300个分离物的分析表明，在日本当前的稻瘟病种群中存在两个离散的谱系。它们被指定为JL1和JL2。有两个谱系与之前在1960年前的采集中发现的五个谱系中的两个相对应，这表明日本稻瘟病种群的结构在1960-1976年间发生了巨大变化。对越南稻瘟病种群进行了Maggy探针DNA指纹分析和致病型分析。这些分析表明，越南北部地区的稻瘟病种群在遗传上比南部地区更具多样性。在这两个群体中，没有发现谱系和小种之间的关系，这表明“谱系排斥假说”不适用于这些群体。对稻瘟病菌、水稻分离物、小麦分离物、Panicum分离物、狗尾草分离物、Eleusine分离物和马唐分离物的6个寄主特异性亚群进行了DNA指纹实验，建立了DNA诊断体系。我们提出了以下公式来区分这些亚群：(1)HindIII+MGR583：鉴定每个群(2)Sali+Maggy：鉴定水稻和狗尾草分离物(3)EcoRV+MGR583：鉴定小麦分离物(4)HindIII+Pot2：鉴定马唐分离物

项目成果

Don et al.: "Population structure of the rice blast pathogen in Vietnam"Ann. Phytopathol. Soc. Jpn.. 65. 475-479 (1999)

Don 等人：“越南稻瘟病病原体的种群结构”Ann。

Kusaba,M., L.D.Don, A.S.Urashima, Y.Eto, Y.Tosa, H.Nakayashiki, M.Yamamoto, and S.Mayama: "Natural infection of wild grass species with rice blast fungus suggested by DNA fingerprinting"Ann. Phytopashol. Soc. Jpn.. 64. 125-128 (1998)

Kusaba，M.，L.D.Don，A.S.Urashima，Y.Eto，Y.Tosa，H.Nakayashiki，M.Yamamoto 和 S.Mayama：“通过 DNA 指纹分析表明稻瘟病菌对野生草种的自然感染”Ann。

Kusaba et al.: "Natural infection of wild grass species with rice blast fungus suggested by DNA fingerprinting"Ann. Phytopathol. Soc. Jpn.. 64. 125-128 (1998)

Kusaba 等人：“DNA 指纹识别表明稻瘟病菌对野生草种的自然感染”Ann。

Don et al.: "Population structure of the rice blast pathogen in Vietnam"Ann.Phytopathol.Soc.Jpn.. 65. 475-479 (1999)

Don 等人：“越南稻瘟病菌的种群结构”Ann.Phytopathol.Soc.Jpn.. 65. 475-479 (1999)

Don et al.: "Population structure of the rice blast funcgen in Japan examined by DNA fingerprinting"Ann.Phytopathol.Soc.Jpn.. 65. 15-24 (1999)

Don等人：“通过DNA指纹法检查日本稻瘟病菌的种群结构”Ann.Phytopathol.Soc.Jpn.. 65. 15-24 (1999)