Endocrine Mechanisms of Ovarian Follicular Development in domestic Animals---the relationships between FSH and Inhibin---

针对纯化的猪卵巢抑制素和牛卵巢抑制素制备了抗血清。我们现在已经建立了RIA系统，可用于测量循环猪、大鼠、奶牛、山羊和妇女外周血血清中的抑制素浓度。在循环猪、大鼠和山羊中，血清抑制素水平在卵泡期较高，在促黄体生成素/卵泡刺激素激增后急剧下降。这些动物在黄体期抑制素水平相对较低。在这些动物的黄体期也发现了一些小的抑制素增加。在排卵期的猪、大鼠和山羊中，抑制素水平的波动在排卵后持续了相当长的时间，这可能反映了卵巢中卵泡的发育和退化。在循环奶牛中，血清抑制素水平在排卵前LH/FSH激增后36 ~ 48小时达到最大值，此时血清黄体酮水平仍然较低。抑制素水平随后下降，并在黄体期保持相对较低水平。除第二次卵泡刺激素激增期间外，循环奶牛的卵泡刺激素与抑制素之间没有明确的关系。在女性的月经周期中，血清抑制素水平在黄体期相当高，这与迄今为止研究的其他哺乳动物不同。这表明在黄体期抑制素的主要来源是黄体，至少在女性中是这样。因此，女性的黄体似乎在功能上不同于其他哺乳动物，因为它与抑制素的产生有关。

Antisera have been raised against purified porcine and bovine ovarian inhibins. We have now established RIA systems that can be used for the measurement of inhibin concentrations in peripheral sera of cyclic pigs, rats, cows, goats, and women. In cycling pigs, rats and goats, serum inhibin levels are high during the follicular phase and them drop sharply after the prevulatory LH/FSH surge. In these animals inhibin levels are relatively low during the luteal phase. Several small inhibin perks are also found during luteal phase in these animals. In Cycling pigs, rats, and goats, fluctuations in inhibin levels that persisted for a fairly long time after ovulation may reflect the development and degeneration of follicules present in the ovary during the period. In Cycling cows, serum inhibin levels reached a maximum 36 to 48 hours after the preovulatory LH/FSH surge, when serum progesterone levels remained low. Inhibin levels then droppedand remained relatively low during the luteal phase. A clear relationship between FSH and inhibin could not be established in cycling cows, except during the period of the second FSH surge. In the menstrual cycles of women, serum inhibin levels were quite high during the luteal phase, in contrast to the other mammals examined thus far. This suggests that a major source of inhibin during the luteal phase is the corpus luteum, at least in women. Therefore, the corpus luteum of women seems to be functionally different from that of the other mammals as it relates to inhibin production.