Development of radioimmunoassay of inhibin and clinical application

已有抗精制精氨酸和牛过牛抑制素的抗血清被提出。我们现在已经建立了RIA系统，可以用来测量循环猪、大鼠、奶牛、山羊和妇女外周血清中抑制素的浓度。在周期猪、大鼠和山羊中，血清抑制素水平在卵泡期处于较高水平，然后在排卵前的促黄体生成素/卵泡刺激素峰值后急剧下降。在这些动物中，黄体期的抑制素水平相对较低。在周期奶牛中，血清抑制素水平在排卵前的黄体生成素/卵泡刺激素峰值后36-48小时达到最大值，此时血清孕酮水平仍然很低。抑制素水平随后下降，并在黄体期保持相对较低的水平。在女性的月经周期中，黄体期的血清抑制素水平相当高，这与到目前为止所研究的其他哺乳动物形成了鲜明对比。这表明黄体期抑制素的主要来源是黄体，至少在女性是这样。用人抑制素特异性放射免疫法测定了190例正常妊娠5~40周孕妇的血浆抑制素浓度。妊娠妇女在整个妊娠期间的平均血浆抑制素浓度明显高于月经周期正常的非妊娠妇女。为了测定抑制素的日释放量，在24小时内每小时采集5名健康青年男性的血样。夜间和夜间的血清浓度相对较低。基础抑制素水平随年龄的变化在20多岁时最高，随年龄增长而降低。对17头超数排卵的日本黑牛的血浆抑制素浓度与胚胎产量的关系进行了研究。发情期抑制素浓度与排卵量呈显著正相关。

Antisera have been raised against purified procine and bovine overian inhibin. We have now established RIA systems that can be use for the measurement of inhibin concentrations in peripheral sera of cyclic pigs, rats, cows, goats and women.1. In cyclic pigs, rats andgoats, serum inhibin levals are high during the follicular phase and then drop sharply after the preovulatory LH/FSH surge. In those animals inhibin levels are relatively low during luteial phase.2. In cycling cows, serum inhibin levelstreached a maximum 36 to 48 hours after the preovulatory LH/FSH surge, when serum progesterone levels remained low. Inhibin levels then dropped and remained relatively low during luteal phase.3. In menstrual cycles of women, serum inhibin levels were quite high during the luteal phase, in contrast to the other mammals examined thus far. This suggests that a majar source of inhibin during the luteal phase is the corpus luteum, at least in women.4. The plasma inhibin concentrations in 190 normal pregnant women at 5-40 weeks gestation were measured by a specific RIA for human inhibin. The average plasma inhibin concentrations in pregnant women throughout pregnancy were much higher than those in nonpregnant women with a normal menstrual cycle.5. To determine the diurnal release of inhibin, blood sample were collected every hour for 24 h, from five healthy young adults men. During the evenign and the night, serum concentations were relatively low. With regard to the age-related changes on basal inhibin levels, the the highest values were observed in the twenties, and lower values were found with aging.6. The relationships between plasma concentrations of inhibin and embryo production were examined in 17 superovulated Japanese Black cows. Significant correlation was observed between the concentration of inhibin at estrus and the number of ovulation.