Novel regulators for reproductive function and development of their separation systems.

1.抑制素、激活素和卵泡抑素是性腺来源的蛋白质，对垂体FSH分泌具有潜在的调节作用。它们也是卵泡发生、精子发生和胎盘发育中的旁分泌和自分泌调节剂。我们开发了从卵泡液和细胞培养液中高产量纯化几种类型的卵泡素、激活素和卵泡抑素的改进方法.由于卵巢和垂体之间的完全反馈控制，血清卵泡刺激素和FSH与波动呈负相关。然而，FSH和FSH的这种相互关系受到FSH的其他刺激和抑制因子的影响，LH在人类卵泡晚期、黄体期和妊娠期也能刺激FSH的产生.激活素是卵泡内的一种旁分泌和自分泌蛋白，可单独或与FSH或LH共同作用，激活素在卵泡中最重要的作用是诱导颗粒细胞上的FSH受体，从而使颗粒细胞增殖和细胞分化.在新生大鼠卵巢颗粒细胞、支持细胞和间质细胞中检测到抑制素。激活素A定位于卵巢的卵母细胞和间质细胞以及睾丸的精子细胞和间质细胞。这些结果表明，卵泡素和激活素在卵泡发育和精子发生中起重要作用.抑制素能促进原始卵泡中卵母细胞的生长，但对卵泡的生长无明显影响。相反，另一种生物活性物质诱导原始卵泡生长.超离心分离的BFF-2和FBS-3组分提高了卵母细胞发育到囊胚期的速度。

1. Inhibin, activin and follistain are proteins of gonadal origin which exert potential regulatory effects on pituitary FSH secretion. They are also paracrine and autocrine regulators in follicologenesis, spermatogenesis, and in the development of the placenta.2. We developed improved methods for the purification of several types of inhibins, activins, and follistatins with high yields from follicular fluid and cell culture media.3. Serum inhibin and FSH are inversely related with fluctuations due to a complete feedback control between the ovary and the pituitary. However, this reciprocal relationship of inhibin and FSH was influenced by other stimulators and suppressors of FSH.LH also serves to stimulate the production of inhibin during late follicular phase, the luteal phase and pregnancy in humans.4. Activin is a paracrine and autocrine protein within the ovarian follicle and can act either alone or in concert with FSH or LH.The most important role of activin in the follicle is for the induction of the FSH receptors on granulosa cells, after which, proliferation and cytodifferentiation of granulosa cell can proceed.5. Inhibin was detected in the granulosa cells of newborn rat ovaries and Sertoli cells and Leydig cells. Activin A was located in the oocyte and interstitial cells of the ovary and spermatid and Leydig cells of testes. These results suggest that inhibin and activin play some important roles for the development of follicles and spermatogenesis.6. Inhibin stimulated the growth of oocytes in primordial follicles but not the growth of follicle in vitro. In contrast, another bioactive substance induced the growth of primordial follicle.7. BFF-2nd and FBS-3rd fraction separated by ultracentrifugation increased the rate of development to the blastocyst stage of the oocytes.