Roles of inhibins, activins, and follistation in reproductive systems.

1)激活素A和白蛋白A用生物素标记，保持其生物活性。用生物素标记的激活素（activin）和抑制素结合蛋白（Inhibin binding protein，INH-BP）代替RI标记的配体进行配体印迹分析。90kDa。通过免疫亲和层析、凝胶层析和高效液相色谱等方法分离纯化，并测定了其分子量为90 kDa的促卵泡生成素、促卵泡生成素、激活素和促卵泡生成素。5）从马卵泡液中成功分离出4种卵泡抑素A和ProαC的分子形式，并鉴定出4种卵泡抑素结合蛋白; 6）从小鼠卵泡抑素样蛋白中鉴定出1种卵泡抑素样蛋白，该蛋白可能是人类卵泡抑素相关基因（FLRG）的小鼠同源物。

1) Activin A and inhibin A were labelled with biotin which remained their bioactivities. We were able to perform ligand blotting analysis by using biotin labelled activin and inhibin instead of RI labelled ligands.2) We fist demonstrated the existence of inhibin binding protein (INH-BP) in bovine follicular fluid.3) INH-BP with molecular weight of 43 kDa. and 90kDa. were isolated by sucsecced chromatographies of immunoafinity chromatography, gel chromatography and HPLC.4) we also determined the existence of inhibins, activins, follistatins and 90 kDa. INH-BP in porcine pituitary by using a variety of chromatographies and immunodetections.5) We succeeded in the isolation of four molecular forms of inhibin A and ProαC, and the identification of four inhibin binding proteins in equine follicular fluid.6) We reported the identification of a mouse follistatin-like protein, which is most likely a mouse homologue of human follistatin-related gene (FLRG).